Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F178 I1 R1 28 89.4 2015306 95.8% 1930663 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,532,298 A→T T15T (ACA→ACT)  femA_3 → Aminoacyltransferase FemA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,532,2980AT98.7% 241.0 / NA 77T15T (ACA→ACT) femA_3Aminoacyltransferase FemA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (25/50);  minor base G (0/1);  total (25/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTA  >  USA300TCH1516_ALE/2532162‑2532421
                                                                                                                                        |                                                                                                                           
gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTactaca                                                                                                                          <  2:279045/140‑1 (MQ=255)
  gtATTTGTAATCAGTTTATGAAAAAAAAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACCACaaa                                                                                                                        <  2:959333/140‑1 (MQ=255)
   tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                       <  1:318791/140‑1 (MQ=255)
   tATGTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTTAAAAATTATAGTTCAATTCAGAGAGCGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACCAAg                                                                                                                       <  1:721661/140‑1 (MQ=255)
    aTTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                      <  2:627592/140‑1 (MQ=255)
       tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtt                                                                                                                   <  1:807713/140‑1 (MQ=255)
       tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAtt                                                                                                                   <  1:659774/140‑1 (MQ=255)
        gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                  >  2:380365/1‑140 (MQ=255)
          aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                <  1:384389/140‑1 (MQ=255)
             cAGTATTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGGCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcac                                                                                                             <  2:863865/140‑1 (MQ=255)
                   ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTaca                                                                                                       <  1:325981/140‑1 (MQ=255)
                    tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                      <  1:785985/140‑1 (MQ=255)
                     gagaaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                     <  1:514588/137‑1 (MQ=255)
                     gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCTGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGGCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCATATTacaca                                                                                                     <  2:740974/140‑1 (MQ=255)
                     gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTGGGATGAGTTCAATTCGTTTTTTACAAAGCATTTTTTTCATTacaca                                                                                                     >  1:85340/1‑140 (MQ=255)
                     gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                     >  1:1005735/1‑140 (MQ=255)
                     gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                     <  2:909106/140‑1 (MQ=255)
                     gATAAAATAAAAAAATAGAATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                     <  2:70278/140‑1 (MQ=255)
                      ataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTAAAATTacaccc                                                                                                    >  2:202800/1‑138 (MQ=255)
                      ataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGCATTTTTCACATTacacac                                                                                                    >  2:908962/1‑140 (MQ=255)
                       taaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAcacaca                                                                                                   >  2:92020/1‑140 (MQ=255)
                              aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                            <  1:51659/140‑1 (MQ=255)
                                 aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                         >  2:358000/1‑140 (MQ=255)
                                  aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                        <  2:365371/140‑1 (MQ=255)
                                  aaTAGCATTATTAATAAATTCAAAAATAATAGTCCAAATCAGAGAGGGATGGGATAAAAAGAACTTTGTAACGTTGACTTCGGATGAATTCAATGCGTTTACTACAAAGCATTCTTAACAGTACACACAAGCAGCTATTc                                                                                        >  2:539591/1‑140 (MQ=255)
                                  aaTAGCATGATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                        <  2:595777/140‑1 (MQ=255)
                                   aTAGCATTAATAATAAATGCAAAAATTATAGTTCAATTCAGAGAGGGATGTGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                       <  2:25777/140‑1 (MQ=255)
                                         ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACaa                                                                                 >  2:428659/1‑140 (MQ=255)
                                              aataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCATATTACACACAATCAGCTATTCATTACAATCATa                                                                            <  2:662277/140‑1 (MQ=255)
                                                   aTTCAAAGATTATCGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTGTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                       <  1:625524/140‑1 (MQ=255)
                                                     tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTAGAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGa                                                                     <  1:668256/140‑1 (MQ=255)
                                                     tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTCCAATCATAGAGTTGa                                                                     <  1:504284/140‑1 (MQ=255)
                                                         aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTa                                                                 <  2:905186/140‑1 (MQ=255)
                                                         aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATATCTAACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGGTTTa                                                                 >  1:432657/1‑140 (MQ=255)
                                                            ttATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaaa                                                              >  2:974848/1‑140 (MQ=255)
                                                            ttATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaaa                                                              >  2:944908/1‑140 (MQ=255)
                                                             tataGTTCAATTCAGAGAGGGATGGGATAAAGTGAACTTTTGAACGGTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAg                                                             <  1:773760/140‑1 (MQ=255)
                                                                aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                          <  1:642677/140‑1 (MQ=255)
                                                                   tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                       <  1:468621/140‑1 (MQ=255)
                                                                   tCAATGCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                       <  2:828424/140‑1 (MQ=255)
                                                                          agtgagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCCATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCCTAGAGTTCATTTAAAAGGCGATGTGCATCt                                                <  2:865163/137‑1 (MQ=255)
                                                                          agggagGGATGGGATAAAATGATCTTTGTAACTTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                <  1:997848/137‑1 (MQ=255)
                                                                          agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                <  2:2611/140‑1 (MQ=255)
                                                                          agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                >  2:767919/1‑140 (MQ=255)
                                                                          agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                <  1:371329/140‑1 (MQ=255)
                                                                           gagagagaTGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                               <  2:689273/140‑1 (MQ=255)
                                                                           gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGAAGAGTTCGATGCTTGTACTATAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                               <  1:189148/140‑1 (MQ=255)
                                                                             gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCTTTACACTCATAGAGTTGGTTTAAAAGGCGTTGTGCAtgttgt                                             >  1:163580/1‑140 (MQ=255)
                                                                                ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                          >  1:289154/1‑140 (MQ=255)
                                                                                ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                          >  1:97307/1‑140 (MQ=255)
                                                                                  atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACACTCATAGAGTTGATTTAAGAGGCGAGNGGCATCGTGTAgggg                                        >  1:1000013/1‑140 (MQ=255)
                                                                                   tgggatAAATTGAACTTTGTAACGTTGACTTCGGATGCGTTCAATGCGTTTACTACAAAGCATTTTTCATATTACACACAATCCGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                       <  2:761758/140‑1 (MQ=255)
                                                                                   tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGCTGATTTAAAAGGCGATGTCCACTTTGCAGGGGt                                       >  1:712756/1‑140 (MQ=255)
                                                                                          aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGAt                                <  2:145591/140‑1 (MQ=255)
                                                                                           aaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                               <  1:92020/140‑1 (MQ=255)
                                                                                           aaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                               <  1:296588/140‑1 (MQ=255)
                                                                                                 cTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATg                         <  1:88453/140‑1 (MQ=255)
                                                                                                   ttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGCTGACAATGGt                       >  1:439190/1‑140 (MQ=255)
                                                                                                     gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                     >  2:30280/1‑140 (MQ=255)
                                                                                                       aaCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAATTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAg                   >  2:3981/1‑140 (MQ=255)
                                                                                                           ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt               <  2:706956/140‑1 (MQ=255)
                                                                                                           ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAATTGAATTAAATATAAATGTGCATTTTGTTGGGGTTAACGATTACTATGGTCATGTGAt               >  2:784907/1‑140 (MQ=255)
                                                                                                            tGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt              <  2:938484/140‑1 (MQ=255)
                                                                                                             gACTTCGGATGAGTTTAAGGCGTTTACTACAAAGCATTTTTCAAATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg             <  2:334653/140‑1 (MQ=255)
                                                                                                             gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGTGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg             <  2:573511/140‑1 (MQ=255)
                                                                                                             gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg             <  2:300737/140‑1 (MQ=255)
                                                                                                             gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg             <  1:896304/140‑1 (MQ=255)
                                                                                                              acggcGGATGAATTCAATGCGGTTACTACAAAGCATTTTTCACATTACAAACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc            <  2:301274/136‑1 (MQ=255)
                                                                                                              aCTTCTGATGAGTTCAATTCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc            <  1:963416/140‑1 (MQ=255)
                                                                                                              aCTTCGGATGAGTTCAATGCGTTTACTACCAACCATGTTTCCCATTACACTCATTCAGCAAGTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAAGGTCAAGTGAGTGc            >  2:211377/1‑140 (MQ=255)
                                                                                                              aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc            <  1:453351/140‑1 (MQ=255)
                                                                                                              aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc            <  2:292394/140‑1 (MQ=255)
                                                                                                                 ttggATGAGTTCAATGCGTTTACGACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCAATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg         <  2:163580/138‑1 (MQ=255)
                                                                                                                   ggttGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt       <  1:810426/137‑1 (MQ=255)
                                                                                                                   ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCCGGTTATTGCAGGAt       <  2:973837/140‑1 (MQ=255)
                                                                                                                   ggATGAATTCACTGCGTTTACTAAAAAGCATTTTTCACATTACACATAATCAGCTAGTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGAAACTTGTATGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt       <  1:195129/140‑1 (MQ=255)
                                                                                                                        aGTTCAATGCGTTTACTACAAAGCATTTTTTACATTACACACAATCAGCTATTCAGTCCAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGCGATTGCAAGATACTTa  >  2:94843/1‑140 (MQ=255)
                                                                                                                        aGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCGTCGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGGAGGAAGCTTa  >  2:70934/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                           
GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTA  >  USA300TCH1516_ALE/2532162‑2532421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A