Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I1 R1	31	90.0	1985866	95.2%	1890544	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,532,298	A→T	T15T (ACA→ACT)	femA_3 →	Aminoacyltransferase FemA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,532,298	0	A	T	96.9%	299.5 / 0.3	99	T15T (ACA→ACT)	femA_3	Aminoacyltransferase FemA
Reads supporting (aligned to +/- strand): ref base A (1/2); new base T (32/63); total (33/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 105 total aligned reads displayed.

GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  USA300TCH1516_ALE/2532162‑2532433

ttgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTActacaa                                                                                                                                     <  1:309398/140‑1 (MQ=255)

gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGGTTGTActacaa                                                                                                                                     >  1:370179/1‑141 (MQ=255)

gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTActacaa                                                                                                                                     <  1:410213/141‑1 (MQ=255)

gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTGTActacaa                                                                                                                                     >  1:369989/1‑141 (MQ=255)

gtgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAATTCAATGCGTTTActacaa                                                                                                                                     >  1:166403/1‑141 (MQ=255)

       tttAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                              <  2:408295/139‑1 (MQ=255)

         tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                            <  2:67048/141‑1 (MQ=255)

         tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                            <  2:590223/141‑1 (MQ=255)

         tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                            <  2:191025/141‑1 (MQ=255)

          aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                           >  2:184496/1‑141 (MQ=255)

          aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                           >  2:448332/1‑141 (MQ=255)

          aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                           >  2:448988/1‑141 (MQ=255)

          aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                           >  2:457246/1‑141 (MQ=255)

          aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTAAATTCATAGAGGGATGGAATAAAATGAACTTTGTAACGTTGACTTCGGATGGGTTCAATGCGCTTAGTACAACGCATTTTTc                                                                                                                           >  2:448971/1‑141 (MQ=255)

                   ttGATAAAATAAAAAAATAGCCTTATGAATTAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  2:411533/141‑1 (MQ=255)

                   ttGATAAAATAAAAAAATAGCATTATTAATAACTTCAAATATTATAGTTCAATTCAGAGAGGGATGGGATAAAATTAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  2:538996/141‑1 (MQ=255)

                   ataaaaaaataaaatAATAGCATTATTAATAAATTCAATATTTACATGACAATGCATATAGATATGGTAAAAAATGAACTTTGTAACGCTCACCTCTTATAAGTTCAACGCGTGCACTACAATCCATTTTTCATACTacac                                                                                                                  <  2:248818/136‑1 (MQ=255)

                   aaaaaaaaaaaaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCATATTacac                                                                                                                  <  2:181222/136‑1 (MQ=255)

                       taaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa                                                                                                              >  1:60552/1‑141 (MQ=255)

                       taaaataaaaAAATAGCATTATTAATAAATTAAAAAATTATAGATCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAAGGTTGACTTCGGAGGAGTTCAATGCGAATACTCCAAAGCATTTTTCACAATAAACACaa                                                                                                              >  2:184497/1‑141 (MQ=255)

                         aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATc                                                                                                            >  1:98668/1‑141 (MQ=255)

                          aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCATAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                           <  1:503334/141‑1 (MQ=255)

                              aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTa                                                                                                       <  2:589743/141‑1 (MQ=255)

                              aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTa                                                                                                       <  1:219544/141‑1 (MQ=255)

                               aaaaaaAGAATAATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt                                                                                                      <  2:591647/141‑1 (MQ=255)

                                aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAAGGTTGACCACGGAGGAGTTCAATGCGTCTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                     >  1:228920/1‑141 (MQ=255)

                                aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                     >  1:215318/1‑141 (MQ=255)

                                aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGAATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                     >  1:659348/1‑141 (MQ=255)

                                aaaaTAGCATTATTAAGAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                     >  1:493307/1‑141 (MQ=255)

                                 aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACATACAATCAGCTATTc                                                                                                    <  2:673361/141‑1 (MQ=255)

                                 aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                                    <  1:74672/141‑1 (MQ=255)

                                 aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                                    <  1:965007/141‑1 (MQ=255)

                                  aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                   >  2:969849/1‑141 (MQ=255)

                                     aGCATTCTATATAACTTCAAAAATAACAGTCCAATTGAGAGAAGGATGAGATAAAAAGAACTTTGTAACGTTGACTTCGGAAGAGTTCAATGCGTTTACAAAAAAGCATTTTGCACATTACAAACAATCAGCTAttaagaa                                                                                                >  2:650235/1‑136 (MQ=255)

                                      gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc                                                                                               >  1:578843/1‑141 (MQ=255)

                                      gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc                                                                                               >  1:987191/1‑141 (MQ=255)

                                      gCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc                                                                                               <  1:904736/141‑1 (MQ=255)

                                        attattAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTACCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACaa                                                                                             <  1:358280/141‑1 (MQ=255)

                                        attattAATAAATTCAAAAATGATAGTTCAAGTCAGAGAGGGATGGAATAAAATGAACCTGGTACCGTTGACTTCGGATGAGTTCGATGCGTTTACGACATAGCATTTTTCACATTACACACAATCAGCTATTCATTACaa                                                                                             <  1:900631/141‑1 (MQ=255)

                                             taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                        <  2:299976/141‑1 (MQ=255)

                                             taataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                        <  2:756907/141‑1 (MQ=255)

                                                aaaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAATTGAACTTTGTACCGTTGCCTTCGGACGAGTTCACTCCGTTTCCTACATAGCTTTGTGCCCATTATACACAATCAGCTATTGACGACAACCATagag                                                                                     <  2:450924/140‑1 (MQ=255)

                                                  aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                   <  1:62347/141‑1 (MQ=255)

                                                        aaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACATTGACTTCGGATGAGTTCAATGCGTTTACTACAAATCATTTTTCACATTACACACAATCCGCTATTCATTACAATCATAGAGTTGATTTa                                                                             >  2:729028/1‑141 (MQ=255)

                                                        aaaaTTATAGTTCAATTCAGAGAGGGATAGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATCCGTTTACTACAAAGCATTTTTCACATTCCACACAAACAGCTATTCATTACAATCATAGAGTTGATTTa                                                                             >  1:44477/1‑141 (MQ=255)

                                                        aaaaATATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGAGGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCCATTCATTACAATCATAGAGTTGATTTa                                                                             >  2:117454/1‑141 (MQ=255)

                                                         aaaTTACAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                            <  2:763257/141‑1 (MQ=255)

                                                         aaaTTACAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                            <  2:31942/141‑1 (MQ=255)

                                                            ttATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTAGACTGCGGAGGAGGTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAg                                                                         >  2:951353/1‑141 (MQ=255)

                                                                 gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                    <  1:395630/141‑1 (MQ=255)

                                                                  ttCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                   <  1:879719/141‑1 (MQ=255)

                                                                  ttCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                   <  2:268941/141‑1 (MQ=255)

                                                                   tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgt                                                                  <  1:267241/141‑1 (MQ=255)

                                                                   ccAATTCAGAGAGGGATAGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgt                                                                  <  2:271405/140‑1 (MQ=255)

                                                                    cAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgtg                                                                 <  2:918620/141‑1 (MQ=255)

                                                                        tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                             <  1:988957/141‑1 (MQ=255)

                                                                           gagagGGATGGAATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                          <  1:180591/141‑1 (MQ=255)

                                                                            agagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGt                                                         >  1:836520/1‑141 (MQ=255)

                                                                                  atgggatCAAATGAATTGTGTGACTTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGTATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  2:944146/141‑1 (MQ=255)

                                                                                  atgggatAAAAGGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGGTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  1:245151/141‑1 (MQ=255)

                                                                                    gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCCCATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTa                                                 >  1:638400/1‑141 (MQ=255)

                                                                                         aaaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGAt                                            <  2:354694/141‑1 (MQ=255)

                                                                                          aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                           <  2:468606/141‑1 (MQ=255)

                                                                                          aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                           <  1:167386/141‑1 (MQ=255)

                                                                                                aCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGATGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGAGGACAATg                                     >  1:888981/1‑141 (MQ=255)

                                                                                                  tttGGAACGTTGTCTTCGTATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGTTGTGCATCTTGTAGGGGTTAAAGATGACAATGGt                                   <  2:234238/141‑1 (MQ=255)

                                                                                                   ttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc                                  <  1:184496/141‑1 (MQ=255)

                                                                                                   ttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc                                  <  1:457246/141‑1 (MQ=255)

                                                                                                   ttGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc                                  <  1:448332/141‑1 (MQ=255)

                                                                                                   ttGTAACGTTGACTTCGGATGAGTTCAAGGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc                                  <  1:448988/141‑1 (MQ=255)

                                                                                                   ttGGAACGTTGACTTCGGATAAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc                                  <  1:448971/141‑1 (MQ=255)

                                                                                                     gTAACGGTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGGATGTTTCACATTACACACAAGCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCCTCTTGTAGGGGTTAAAGATGACAATGGTCaa                                <  2:413548/141‑1 (MQ=255)

                                                                                                      tAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGACGCCAATGGCCAAg                               <  2:578843/141‑1 (MQ=255)

                                                                                                             gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTCTTCATTACAATCATAGAGTTGATTAAAAAGGCGATGTGCATCTTGTAGGGTTTAAAGATGACAATGGTCAAGTGATTGc                        <  1:638630/141‑1 (MQ=255)

                                                                                                             gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCTTAGAGTTGATTTAAAAGGCGATGTGTATCTTGTATGGGTTCAAGAGTATAATGGTCAAGTGATTGc                        <  1:728431/141‑1 (MQ=255)

                                                                                                             gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc                        <  2:590254/141‑1 (MQ=255)

                                                                                                               cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACATAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGATCAAGTGATTGCAg                      <  2:675002/141‑1 (MQ=255)

                                                                                                               cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAg                      <  1:20838/141‑1 (MQ=255)

                                                                                                                ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                     >  2:122475/1‑141 (MQ=255)

                                                                                                                  cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTTTTTTTTTTCTCAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTGCAGGGATTGCAgggg                   >  2:880093/1‑139 (MQ=255)

                                                                                                                  cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                   >  2:593755/1‑141 (MQ=255)

                                                                                                                   ggATGAGTTCAATGCGTTTAATACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                  >  1:600052/1‑141 (MQ=255)

                                                                                                                       gacttCAAGCCGTTTATTACAAGGCATTTTTTACATTACACACAATCCGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCGTGTATGGGTTAAAGATGACAATGGTCAAGTGATTGCATGATGCTTa              <  2:486339/138‑1 (MQ=255)

                                                                                                                       gAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa              <  1:502732/141‑1 (MQ=255)

                                                                                                                        aGTTCAATGCGTTTACTACAAAGCATTTTTCACATCACACACAATCAGCTCTTCCTTACAACCATAGAGTTGATTTAAAAGGCGATGTGCACCTTGTAGAGGTTAAAGCTGACAATGGTCACGTGATTGCAGGATGCTTAt             >  2:389790/1‑141 (MQ=255)

                                                                                                                        aGTTAAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt             <  2:686483/141‑1 (MQ=255)

                                                                                                                         gTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATCGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt            <  2:713981/141‑1 (MQ=255)

                                                                                                                         gTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCCTCTTTTAGGGGTTAAAGATGACAATGGTCAAGTGATTTCACGATGCTTAtt            <  2:208819/141‑1 (MQ=255)

                                                                                                                            cAATTCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCATGATGCTTATTGAc         <  2:376735/141‑1 (MQ=255)

                                                                                                                            cAATGCTTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAc         <  2:224159/141‑1 (MQ=255)

                                                                                                                             aaTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACa        >  1:12102/1‑141 (MQ=255)

                                                                                                                             aaTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACa        <  2:145132/141‑1 (MQ=255)

                                                                                                                             aaTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGATGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGTTTGCGGGTTGCTTTTTGACa        >  1:100860/1‑141 (MQ=255)

                                                                                                                              aTGCGTTTACTACACAGCATTTTCCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg       <  1:555236/141‑1 (MQ=255)

                                                                                                                              aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg       >  1:198497/1‑141 (MQ=255)

                                                                                                                               tGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGa      <  1:850246/141‑1 (MQ=255)

                                                                                                                                 cGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg    <  1:969849/141‑1 (MQ=255)

                                                                                                                                  gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTGAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgg   >  2:65974/1‑140 (MQ=255)

                                                                                                                                  gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgc   >  1:22168/1‑141 (MQ=255)

                                                                                                                                   tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca  <  1:907585/141‑1 (MQ=255)

GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  USA300TCH1516_ALE/2532162‑2532433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A