Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F21 I0 R1
|
2169 |
63.0 |
3952800 |
76.2% |
3012033 |
62.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,900,258 |
G→A |
29.6% |
A928V (GCT→GTT) |
recB ← |
exonuclease V (RecBCD complex), beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,900,258 | 0 | G | A | 29.6%
| 101.4
/ 51.6
| 71 | A928V (GCT→GTT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (26/24); new base A (8/13); total (34/37) |
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGCT > minE/1900188‑1900328
|
cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:361916/1‑71 (MQ=255)
ggTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:1288992/1‑70 (MQ=255)
tGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCAt > 1:1652640/1‑71 (MQ=255)
aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc < 1:469138/70‑1 (MQ=255)
cACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACa > 1:1345197/1‑71 (MQ=255)
aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACAt > 1:2544009/1‑71 (MQ=255)
aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACAt > 1:2922483/1‑71 (MQ=255)
cgcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATc > 1:3127706/1‑70 (MQ=255)
cgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAg > 1:2956039/1‑71 (MQ=255)
cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:3276163/71‑1 (MQ=255)
cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:2318/71‑1 (MQ=255)
cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:372064/71‑1 (MQ=255)
cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGcc < 1:11998/71‑1 (MQ=255)
ggAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCg < 1:2235723/71‑1 (MQ=255)
gAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGa < 1:1169303/71‑1 (MQ=255)
aaaCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAg > 1:2488333/1‑71 (MQ=255)
aaaCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAg > 1:3314224/1‑71 (MQ=255)
aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:976367/1‑70 (MQ=255)
aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:2366136/1‑70 (MQ=255)
aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:971639/1‑70 (MQ=255)
aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:1462274/1‑70 (MQ=255)
aCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTAGCAACGCCTGCAGCATCGACATCCAGCCGAgg > 1:3119208/1‑70 (MQ=255)
cTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCa < 1:1685675/71‑1 (MQ=255)
gATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCAt > 1:174729/1‑70 (MQ=255)
aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATc > 1:3265652/1‑70 (MQ=255)
aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATc > 1:2171646/1‑70 (MQ=255)
aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGa > 1:1021553/1‑64 (MQ=255)
tgtgGTGTTAACGTCGTTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGa < 1:2159110/63‑1 (MQ=255)
tgtgGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGa < 1:594362/63‑1 (MQ=255)
tggtgTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCaaa < 1:2691484/70‑1 (MQ=255)
tggtgTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCaaa < 1:2265630/70‑1 (MQ=255)
ggtgTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc > 1:3908236/1‑71 (MQ=255)
gtgtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATcc < 1:220629/71‑1 (MQ=255)
tgtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt < 1:2146882/71‑1 (MQ=255)
gtTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGa > 1:203510/1‑57 (MQ=255)
ttAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcagca < 1:2817580/41‑1 (MQ=255)
ttAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTg < 1:3303861/70‑1 (MQ=255)
tAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc < 1:3097454/66‑1 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg > 1:2392536/1‑70 (MQ=255)
aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg < 1:782903/70‑1 (MQ=255)
gTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:2418723/64‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCg < 1:3121309/70‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCg < 1:3101828/70‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCGAATCCTGGGCGATACCGt < 1:3556382/71‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:3279373/71‑1 (MQ=255)
ttcttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:1715766/71‑1 (MQ=255)
tcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt > 1:1637013/1‑70 (MQ=255)
tcttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGc < 1:3498359/62‑1 (MQ=255)
tcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAc > 1:1603009/1‑70 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGa < 1:2467533/41‑1 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAcc < 1:1350217/70‑1 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAc < 1:3843017/69‑1 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCa > 1:1409/1‑71 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCa > 1:2632049/1‑71 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCa > 1:2059225/1‑71 (MQ=255)
cAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCa > 1:1608748/1‑71 (MQ=255)
cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGc < 1:2105720/71‑1 (MQ=255)
cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGc < 1:3850063/71‑1 (MQ=255)
cgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGc > 1:903526/1‑71 (MQ=255)
acgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTg < 1:3023548/71‑1 (MQ=255)
cgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGTCGATACCGTGACCACGCTg < 1:496617/70‑1 (MQ=255)
cgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGt < 1:1421276/71‑1 (MQ=255)
cTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt < 1:2025463/46‑1 (MQ=255)
cTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCt < 1:2623486/46‑1 (MQ=255)
cTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTg > 1:1185949/1‑71 (MQ=255)
cTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCaaa < 1:772811/42‑1 (MQ=255)
gCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGtt > 1:42441/1‑67 (MQ=255)
gCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCa < 1:1297523/70‑1 (MQ=255)
aaCGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAAc > 1:1984538/1‑71 (MQ=255)
aCGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAAc > 1:2516469/1‑70 (MQ=255)
cGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCaatcg > 1:678450/1‑67 (MQ=255)
gCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAAc > 1:1652488/1‑68 (MQ=255)
cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag > 1:2957942/1‑71 (MQ=255)
cTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGa < 1:699393/50‑1 (MQ=255)
gcagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTg > 1:1404971/1‑59 (MQ=255)
gcaacaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAACACGCTGTTGCAAACCagag > 1:1168939/1‑69 (MQ=255)
cagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAAcc > 1:3456724/1‑64 (MQ=255)
cagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAg < 1:2115534/71‑1 (MQ=255)
agcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGc > 1:1166110/1‑71 (MQ=255)
gcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGCt < 1:1081733/71‑1 (MQ=255)
|
CGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGCT > minE/1900188‑1900328
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
