Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R1 2169 63.0 3952800 76.2% 3012033 62.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,132 C→A 19.2% A437A (GCG→GCT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1320CA19.2% 219.2 / 26.9 83A437A (GCG→GCT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (32/35);  new base A (11/5);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

AATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAA  >  minE/1933062‑1933202
                                                                      |                                                                      
aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                                        >  1:2933978/1‑71 (MQ=255)
 aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                                                                       >  1:3277849/1‑71 (MQ=255)
 aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                                        <  1:3570258/70‑1 (MQ=255)
      cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa                                                                  >  1:646527/1‑71 (MQ=255)
       aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa                                                                  <  1:1915100/70‑1 (MQ=255)
       aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt                                                                 >  1:384461/1‑71 (MQ=255)
       aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGAt                                                                 >  1:3545562/1‑71 (MQ=255)
        tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATc                                                                >  1:3267113/1‑71 (MQ=255)
        tCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATc                                                                <  1:610835/71‑1 (MQ=255)
          gTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                                                      >  1:2320695/1‑63 (MQ=255)
          gTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                                                      >  1:1102500/1‑63 (MQ=255)
           tAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTc                                                             >  1:1165781/1‑71 (MQ=255)
           tAGAACATCGCTTCGTCGTCACCTGCGGCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTc                                                             <  1:3782300/71‑1 (MQ=255)
            aGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGa                                                                  <  1:581029/65‑1 (MQ=255)
            aGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct                                                            >  1:1747131/1‑70 (MQ=255)
            aGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTcc                                                            >  1:335065/1‑71 (MQ=255)
              aaCATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCtgc                                                          <  1:1624122/71‑1 (MQ=255)
               aCATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCtgcg                                                         <  1:1128480/71‑1 (MQ=255)
               aCATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCtgc                                                          <  1:3332342/70‑1 (MQ=255)
               aCATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCtgc                                                          <  1:139504/70‑1 (MQ=255)
               aCATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcgcg                                                         <  1:2525895/71‑1 (MQ=255)
                 aTCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                                           <  1:1610090/67‑1 (MQ=255)
                 aTCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                                           <  1:1647274/67‑1 (MQ=255)
                  tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                                           <  1:1253598/66‑1 (MQ=255)
                     cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa                                                                  <  1:3738862/56‑1 (MQ=255)
                        cgtcgtCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGc                                                >  1:2262898/1‑71 (MQ=255)
                         gtcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGc                                                <  1:1384504/70‑1 (MQ=255)
                         gtcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt                                               <  1:2906310/71‑1 (MQ=255)
                               aCCTGCGTCTTTCTCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTa                                          >  1:3859545/1‑70 (MQ=255)
                               aCCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTaa                                         <  1:3903421/71‑1 (MQ=255)
                               aCCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTa                                          <  1:2483047/70‑1 (MQ=255)
                               aCCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGAGTCATTCAGCTCGCTa                                          <  1:2476857/70‑1 (MQ=255)
                                ccTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTaaa                                        <  1:3922048/71‑1 (MQ=255)
                                 cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                       >  1:1897727/1‑71 (MQ=255)
                                   tcGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTa                                          <  1:2827202/65‑1 (MQ=255)
                                   gCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc                                     >  1:2650001/1‑71 (MQ=255)
                                   gCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc                                      >  1:1698278/1‑70 (MQ=255)
                                     gTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCa                                                  >  1:1552691/1‑56 (MQ=255)
                                     gTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                                   <  1:2317057/71‑1 (MQ=255)
                                      tCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGtt                                  <  1:3289036/71‑1 (MQ=255)
                                       cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGtt                                  <  1:2592423/70‑1 (MQ=255)
                                       cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa                                 >  1:242409/1‑71 (MQ=255)
                                         ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                               >  1:313799/1‑71 (MQ=255)
                                          tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                               <  1:1573752/70‑1 (MQ=255)
                                          tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc                                <  1:3486576/69‑1 (MQ=255)
                                           cgcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGa                             >  1:1126789/1‑71 (MQ=255)
                                            gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt                            >  1:2037557/1‑71 (MQ=255)
                                              ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAtt                           <  1:403066/70‑1 (MQ=255)
                                               ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtt                                                    >  1:2340006/3‑44 (MQ=12)
                                               ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtt                                                    >  1:3865378/3‑44 (MQ=12)
                                               gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTc                         <  1:2295148/71‑1 (MQ=255)
                                                taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCa                        >  1:1499791/2‑71 (MQ=255)
                                                cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                                                    <  1:1707248/43‑1 (MQ=255)
                                                cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                       <  1:1790523/56‑1 (MQ=255)
                                                cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                       <  1:3034267/56‑1 (MQ=255)
                                                 aaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAAcca                                    >  1:1929449/1‑57 (MQ=255)
                                                  aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                       <  1:2360341/54‑1 (MQ=255)
                                                  aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACg                      <  1:573730/71‑1 (MQ=255)
                                                   ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGa                     >  1:3502104/1‑71 (MQ=255)
                                                    cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac                    <  1:1682021/71‑1 (MQ=255)
                                                     tGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                          <  1:458023/64‑1 (MQ=255)
                                                      gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca                  >  1:3153879/1‑71 (MQ=255)
                                                      gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca                  >  1:1219652/1‑71 (MQ=255)
                                                       ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac                 >  1:1321896/2‑71 (MQ=255)
                                                       ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGa                     >  1:1094129/2‑67 (MQ=255)
                                                       ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGa                     >  1:1303462/2‑67 (MQ=255)
                                                       aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac                 >  1:1386299/1‑71 (MQ=255)
                                                       aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacc                   <  1:1249779/69‑1 (MQ=255)
                                                       aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacc                   <  1:3179727/69‑1 (MQ=255)
                                                        tCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                >  1:438114/1‑71 (MQ=255)
                                                        tCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc                >  1:433552/1‑71 (MQ=255)
                                                        tCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac                 >  1:1477415/1‑70 (MQ=255)
                                                        tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCg                              >  1:1966535/1‑57 (MQ=255)
                                                        tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac                 >  1:3856698/1‑70 (MQ=255)
                                                         ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccacc                >  1:222669/1‑70 (MQ=255)
                                                         ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca                  >  1:2677720/1‑68 (MQ=255)
                                                          cAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGa                     >  1:2628038/1‑64 (MQ=255)
                                                           aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt             >  1:1174807/1‑71 (MQ=255)
                                                              aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca                  >  1:2579610/1‑63 (MQ=255)
                                                              aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca                  >  1:2291577/1‑63 (MQ=255)
                                                              aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg            >  1:2422718/1‑69 (MQ=255)
                                                               aGCGTTGCGCCTGATCTTCCGCGTCATTTAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag         <  1:3100201/71‑1 (MQ=255)
                                                               aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag         <  1:588970/71‑1 (MQ=255)
                                                               aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg            >  1:811197/1‑68 (MQ=255)
                                                               aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg            >  1:2028881/1‑68 (MQ=255)
                                                               aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg            >  1:2820078/1‑68 (MQ=255)
                                                                  gTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga           <  1:1899667/66‑1 (MQ=255)
                                                                   ttGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACt     <  1:2981290/71‑1 (MQ=255)
                                                                     gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGa                             >  1:1412504/1‑45 (MQ=255)
                                                                     gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac                    >  1:969420/1‑54 (MQ=255)
                                                                     gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc    >  1:2710334/1‑70 (MQ=255)
                                                                     gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc    >  1:1515216/1‑70 (MQ=255)
                                                                      cgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCaa  <  1:3357953/71‑1 (MQ=255)
                                                                      |                                                                      
AATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAA  >  minE/1933062‑1933202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A