Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F21 I0 R2 370 72.1 3738297 91.5% 3420541 62.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,126 G→A 6.2% R439R (CGC→CGT)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,1260GA 6.2% 264.8 / 5.7 81R439R (CGC→CGT) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (36/40);  new base A (2/3);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAG  >  minE/1933058‑1933195
                                                                    |                                                                     
aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                                                      <  1:3032348/70‑1 (MQ=255)
aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc                                                                      <  1:3302403/70‑1 (MQ=255)
aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCg                                                                     >  1:2173432/1‑71 (MQ=255)
 cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                                                    >  1:1458125/1‑71 (MQ=255)
 cGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt                                                                    >  1:362217/1‑71 (MQ=255)
 cGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt                                                                    <  1:145945/71‑1 (MQ=255)
 cGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt                                                                    <  1:32075/71‑1 (MQ=255)
   tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTg                                                                  <  1:203329/71‑1 (MQ=255)
   tAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTg                                                                  >  1:1401504/1‑71 (MQ=255)
    aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                                 >  1:3236910/1‑71 (MQ=255)
    aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc                                                                 >  1:533772/1‑71 (MQ=255)
     aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg                                                                >  1:3440040/1‑71 (MQ=255)
         tCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                                             >  1:3617230/1‑70 (MQ=255)
          cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                                               <  1:583430/67‑1 (MQ=255)
          cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                                               >  1:579646/1‑67 (MQ=255)
          cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcgc                                                               >  1:1914762/1‑67 (MQ=255)
          cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc                                                              <  1:2997881/68‑1 (MQ=255)
          cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                                             <  1:1402321/69‑1 (MQ=255)
          cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt                                                             <  1:2874433/69‑1 (MQ=255)
           aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg                                                            <  1:1111011/69‑1 (MQ=255)
           aTCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg                                                            <  1:555642/69‑1 (MQ=255)
               tAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg                                                            <  1:1001371/65‑1 (MQ=255)
               tAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTc                                                      >  1:1351967/1‑71 (MQ=255)
                 gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct                                                     <  1:620064/70‑2 (MQ=255)
                 gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct                                                     <  1:1929007/70‑2 (MQ=255)
                 gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct                                                     <  1:3668796/70‑2 (MQ=255)
                 gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                                    <  1:2842864/71‑1 (MQ=255)
                 gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg                                                    >  1:132471/1‑71 (MQ=255)
                    cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAg                                                                       >  1:459341/1‑49 (MQ=255)
                      tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa                                               <  1:1515315/71‑1 (MQ=255)
                        gCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                                             <  1:3721822/71‑1 (MQ=255)
                         cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                                             <  1:3697729/70‑1 (MQ=255)
                         cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                                             <  1:1217093/70‑1 (MQ=255)
                           tcgtcgTCACTTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAg                                          >  1:697240/1‑71 (MQ=255)
                           tcgtcgTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAg                                          >  1:1412372/1‑71 (MQ=255)
                            cgtcgtCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCa                                           <  1:1258963/69‑1 (MQ=255)
                             gtcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGc                                         <  1:2839302/70‑1 (MQ=255)
                             gtcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGc                                         <  1:3617753/70‑1 (MQ=255)
                             gtcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGc                                         <  1:2069330/70‑1 (MQ=255)
                             gtcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGc                                         <  1:3613428/70‑1 (MQ=255)
                                gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc                                     <  1:1010574/71‑1 (MQ=255)
                                gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc                                     >  1:1750396/1‑71 (MQ=255)
                                gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc                                     <  1:1563464/71‑1 (MQ=255)
                                 tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGt                                                                    <  1:802717/39‑1 (MQ=255)
                                 tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc                                     >  1:3115832/1‑70 (MQ=255)
                                  cACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg                                                            <  1:2378735/46‑1 (MQ=255)
                                     cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                >  1:3469767/1‑71 (MQ=255)
                                     cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                >  1:754459/1‑71 (MQ=255)
                                     cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                <  1:2981397/71‑1 (MQ=255)
                                     cTGCGTCTTTCGAGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg                                >  1:1614046/1‑71 (MQ=255)
                                      tGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTc                                                      <  1:3439401/48‑1 (MQ=255)
                                       gCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc                              >  1:636305/1‑71 (MQ=255)
                                         gTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                            >  1:2522874/1‑71 (MQ=255)
                                         gTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt                            >  1:1159005/1‑71 (MQ=255)
                                                gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt                     <  1:2583566/71‑1 (MQ=255)
                                                  ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                   <  1:2997836/71‑1 (MQ=255)
                                                  ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                   >  1:297682/1‑71 (MQ=255)
                                                   gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt                   >  1:1889561/1‑70 (MQ=255)
                                                      aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTaaa                                 <  1:347413/53‑1 (MQ=255)
                                                      aCCTGATCCAGGAAGCGTTGCGCCTCATCTTCCGCGTCAtt                                             >  1:864233/1‑41 (MQ=255)
                                                       ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGGGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACg               <  1:1656769/70‑1 (MQ=255)
                                                       ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt                                             <  1:2395289/40‑1 (MQ=255)
                                                       ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGa              >  1:49977/1‑71 (MQ=255)
                                                        cTGATCCAGGAGGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac             >  1:1029600/1‑71 (MQ=255)
                                                        cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTa                                   <  1:2341047/49‑1 (MQ=255)
                                                         tGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac             >  1:1630484/1‑70 (MQ=255)
                                                         tGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac             >  1:1201758/1‑70 (MQ=255)
                                                          gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca           >  1:3511228/1‑71 (MQ=255)
                                                          gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac             >  1:2149550/1‑69 (MQ=255)
                                                          gATCCAGGAAGCGTTGCGCCTGATCTTCAGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca           >  1:2737920/1‑71 (MQ=255)
                                                             ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCa                                           >  1:2933016/1‑36 (MQ=255)
                                                             ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCa                                           >  1:1162975/1‑36 (MQ=255)
                                                              cAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCaa       <  1:2061484/71‑1 (MQ=255)
                                                               aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt      >  1:3132694/1‑71 (MQ=255)
                                                               aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt      >  1:1161547/1‑71 (MQ=255)
                                                               aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt      <  1:1240995/71‑1 (MQ=255)
                                                               aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt      >  1:1547185/1‑71 (MQ=255)
                                                               aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt      <  1:180919/71‑1 (MQ=255)
                                                                ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg     <  1:659870/71‑1 (MQ=255)
                                                                 gAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga    <  1:1668624/71‑1 (MQ=255)
                                                                   aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag  <  1:1273394/71‑1 (MQ=255)
                                                                   aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg     >  1:1951814/1‑68 (MQ=255)
                                                                    gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                        <  1:1444364/48‑1 (MQ=255)
                                                                    gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc                        <  1:2873177/48‑1 (MQ=255)
                                                                    |                                                                     
ACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAG  >  minE/1933058‑1933195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A