Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
100% |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 146.5
/ NA
| 51 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (33/18); total (33/18) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563523‑4563654
|
gTCGGCAGCAGGTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:2419310/71‑1 (MQ=255)
gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:2696401/71‑1 (MQ=255)
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt < 1:547474/71‑1 (MQ=255)
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGAAACGCCATTTTACt > 1:4099933/1‑71 (MQ=255)
ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAAGGCCATTTTACTGTCGCCCAt > 1:641148/1‑71 (MQ=255)
ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc < 1:770635/69‑1 (MQ=255)
gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:4527225/69‑1 (MQ=255)
cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg < 1:652548/71‑1 (MQ=255)
tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:3358199/1‑58 (MQ=255)
tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:3552578/1‑58 (MQ=255)
tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:2815138/1‑58 (MQ=255)
tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:249295/1‑58 (MQ=255)
tGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt > 1:631765/1‑58 (MQ=255)
tGATCGGCACATCGCAGTTTTCAAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg > 1:3076/1‑70 (MQ=255)
aTCGGCACATCGGAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAGTGTCGGCCATGTgg < 1:233460/69‑1 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:4489704/1‑71 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:1514928/1‑71 (MQ=255)
cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg > 1:679904/1‑66 (MQ=255)
ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca < 1:2511349/70‑1 (MQ=255)
aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTg > 1:4578573/1‑70 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGt > 1:2612106/1‑47 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:1015876/1‑71 (MQ=255)
cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc < 1:2377153/71‑1 (MQ=255)
gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg < 1:3513076/71‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:3608906/38‑1 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:1481043/1‑37 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:4452210/1‑38 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc > 1:1089566/1‑38 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:882591/52‑1 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:582772/1‑71 (MQ=255)
tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt < 1:2462634/70‑1 (MQ=255)
agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc < 1:344153/71‑1 (MQ=255)
gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:1662463/43‑1 (MQ=255)
tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg < 1:4264392/71‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:2848955/45‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa < 1:1280990/71‑1 (MQ=255)
aaaTAGGCTGTAACTCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACC‑CCGAGCaaa > 1:2443218/1‑70 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:3111758/1‑39 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:53801/42‑1 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:1736978/1‑71 (MQ=255)
gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:2694362/38‑1 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:690679/1‑64 (MQ=255)
gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCa < 1:3552112/60‑1 (MQ=255)
aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg > 1:4090901/1‑71 (MQ=255)
aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg > 1:1814469/1‑71 (MQ=255)
aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg > 1:2937517/1‑71 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:192545/1‑52 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:2313128/1‑52 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgcc > 1:3396041/1‑52 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:225543/1‑48 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:4168850/1‑71 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:3227959/1‑71 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa > 1:839923/1‑52 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGa > 1:1092235/1‑52 (MQ=255)
|
GTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563523‑4563654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
