Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
100% |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 127.5
/ NA
| 43 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (18/25); total (18/25) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563524‑4563654
|
tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:2854277/69‑1 (MQ=255)
tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:2652029/69‑1 (MQ=255)
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:101797/1‑69 (MQ=255)
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:3126402/1‑69 (MQ=255)
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:2210892/69‑1 (MQ=255)
ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt > 1:2948428/1‑58 (MQ=255)
gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAGTGTCg > 1:1387983/1‑64 (MQ=255)
gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt > 1:997492/1‑69 (MQ=255)
tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:1377255/51‑1 (MQ=255)
cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:2199710/63‑1 (MQ=255)
cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGATGTAACGCCATTTTACTGTCGCCCATGTgg < 1:2031203/67‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:2223213/69‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:1109192/69‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:3188610/69‑1 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGAc < 1:1052322/69‑1 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt > 1:3512304/1‑36 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:598362/1‑37 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:1968352/1‑37 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:3379035/1‑37 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg > 1:2118114/1‑69 (MQ=255)
tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:766091/69‑1 (MQ=255)
tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:677608/69‑1 (MQ=255)
tCATAAATAGGCGGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:289039/69‑1 (MQ=255)
cATAAATAGTCTGTAACGCCATTTTACTGTCGCCCa < 1:1620650/36‑1 (MQ=37)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:245708/36‑1 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:2179628/41‑1 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:2011859/41‑1 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:3182403/41‑1 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:112616/41‑1 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:3707962/1‑45 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:1025019/1‑45 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:2563563/1‑45 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:2372202/1‑44 (MQ=255)
aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:2282746/1‑44 (MQ=255)
aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:1853031/43‑1 (MQ=255)
gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCg > 1:3004709/1‑68 (MQ=255)
cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:2804896/69‑1 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:1804557/1‑46 (MQ=255)
tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCa < 1:1647541/69‑1 (MQ=255)
aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:410888/1‑46 (MQ=255)
aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:1152743/1‑46 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCaa > 1:2257801/1‑66 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg < 1:1997806/68‑1 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg < 1:1793327/68‑1 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg < 1:760349/68‑1 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg < 1:1027588/68‑1 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg < 1:2091030/69‑1 (MQ=255)
gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg < 1:590012/69‑1 (MQ=255)
|
TCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563524‑4563654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
