Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,563,589 |
T→A |
K207M (AAG→ATG) |
iadA ← |
isoaspartyl dipeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0%
| 135.1
/ NA
| 45 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (20/25); total (20/25) |
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563522‑4563654
|
ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:4345284/71‑1 (MQ=255)
gTCGGGAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:2878056/71‑1 (MQ=255)
gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:4322746/71‑1 (MQ=255)
gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:1775741/71‑1 (MQ=255)
ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAc < 1:813938/70‑1 (MQ=255)
agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc > 1:4499338/1‑71 (MQ=255)
agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAATGTCGcc > 1:1292923/1‑71 (MQ=255)
gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc < 1:3832673/71‑1 (MQ=255)
cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:2221846/71‑1 (MQ=255)
cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:1380950/71‑1 (MQ=255)
cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:3570225/71‑1 (MQ=255)
cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:3221084/71‑1 (MQ=255)
aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa < 1:3207992/71‑1 (MQ=255)
cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:2533027/1‑71 (MQ=255)
ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:455566/71‑1 (MQ=255)
ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:4406259/71‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:4239806/71‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:3105797/71‑1 (MQ=255)
acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:3416696/71‑1 (MQ=255)
tCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:241757/68‑1 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa > 1:408829/1‑53 (MQ=255)
gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc > 1:4075192/1‑71 (MQ=255)
cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc < 1:1508954/71‑1 (MQ=255)
cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc < 1:336817/71‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:3399253/38‑1 (MQ=255)
ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:2637619/38‑1 (MQ=255)
ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:861319/1‑71 (MQ=255)
cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa < 1:220136/70‑1 (MQ=255)
cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa < 1:1881263/70‑1 (MQ=255)
gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:4549417/51‑1 (MQ=255)
tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:1737678/1‑71 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:706735/1‑70 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:4586615/1‑70 (MQ=255)
cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:1660890/1‑70 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac < 1:3307724/42‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAac < 1:469968/42‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa < 1:3924572/71‑1 (MQ=255)
tAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa < 1:1608994/71‑1 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg > 1:2908502/1‑37 (MQ=255)
aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:1003586/1‑71 (MQ=255)
tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCg > 1:3748153/1‑71 (MQ=255)
aGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGc > 1:470998/1‑71 (MQ=255)
cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:4468144/39‑1 (MQ=255)
cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:1933783/39‑1 (MQ=255)
cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGt < 1:1005145/39‑1 (MQ=255)
tAACGCCATTTTACTGTCGCCCAAGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgc > 1:1308914/1‑71 (MQ=255)
aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg > 1:1844774/1‑71 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc > 1:2215424/1‑48 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:1927004/1‑71 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:540615/1‑71 (MQ=255)
aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg > 1:2739848/1‑71 (MQ=255)
|
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563522‑4563654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
