Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,591,162 |
C→A |
V112V (GTG→GTT) |
hsdR ← |
endonuclease R |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,591,162 | 0 | C | A | 100.0%
| 157.0
/ NA
| 52 | V112V (GTG→GTT) | hsdR | endonuclease R |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (23/29); total (23/29) |
ACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGA > W3110S.gb/4591097‑4591229
|
aCAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGc < 1:1801186/71‑1 (MQ=255)
cAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGcc > 1:1261292/1‑71 (MQ=255)
cGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTAcc < 1:2382570/71‑1 (MQ=255)
ggAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTTcc < 1:1745732/70‑1 (MQ=255)
gTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCTCCTGGt < 1:53443/57‑1 (MQ=255)
tCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTa < 1:3845877/71‑1 (MQ=255)
cAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGt < 1:2968731/67‑1 (MQ=255)
aGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAAttt < 1:927035/71‑1 (MQ=255)
ggCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTaa > 1:2389959/1‑67 (MQ=255)
cacaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTtgt > 1:2351559/1‑70 (MQ=255)
acaTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGa < 1:4675918/71‑1 (MQ=255)
aTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa < 1:2707932/71‑1 (MQ=255)
aTCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa < 1:2212418/71‑1 (MQ=255)
tCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaat > 1:4269730/1‑69 (MQ=255)
tCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa > 1:4273112/1‑70 (MQ=255)
tCTGGGCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa > 1:2772684/1‑70 (MQ=255)
ggCATCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACa < 1:445980/70‑1 (MQ=255)
aTCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa > 1:764642/1‑63 (MQ=255)
tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGaaa < 1:3878469/62‑1 (MQ=255)
tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag < 1:2669256/71‑1 (MQ=255)
tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag < 1:598110/71‑1 (MQ=255)
tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag < 1:3648955/71‑1 (MQ=255)
tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACa < 1:4028940/66‑1 (MQ=255)
tCGTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACa < 1:2908071/66‑1 (MQ=255)
tCGTTGAGATCGTTATGATATTAGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag < 1:4395520/71‑1 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGGCTGGTTACCaa > 1:4243327/1‑41 (MQ=38)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATa > 1:4565574/1‑62 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATa > 1:4190123/1‑62 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACaga > 1:4561471/1‑66 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACaga > 1:3893940/1‑66 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAgg < 1:141180/70‑1 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:3316394/1‑71 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:2021267/1‑71 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:4396856/1‑71 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:1254367/1‑71 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:426693/1‑71 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:122234/1‑71 (MQ=255)
gTTGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa > 1:1052732/1‑71 (MQ=255)
ttGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGa < 1:1101971/70‑1 (MQ=255)
ttGAGATCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAt < 1:3090644/71‑1 (MQ=255)
gagaTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtg < 1:2912929/70‑1 (MQ=255)
agaTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtgtt < 1:1567866/71‑1 (MQ=255)
aTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag < 1:4433714/63‑1 (MQ=255)
aTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtgttgt < 1:3212059/71‑1 (MQ=255)
aTCGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGAtgttgt < 1:56308/71‑1 (MQ=255)
cGTTATGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGtca > 1:4508596/1‑71 (MQ=255)
aTGATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATcaa > 1:385884/1‑71 (MQ=255)
gATATTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag < 1:2353710/55‑1 (MQ=255)
tatTCGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACagagag < 1:2829167/53‑1 (MQ=255)
atTCGTGAACCGCC‑GGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAgg > 1:3155786/1‑71 (MQ=255)
cGTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGc > 1:3863301/1‑70 (MQ=255)
gTGAACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGa < 1:3436559/71‑1 (MQ=255)
|
ACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGGCGTAATTTGTGAAATACAGAGAGGATGTTGTCATCAACAAAGGCGA > W3110S.gb/4591097‑4591229
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
