Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I2 R1
|
330 |
0.0 |
2544031 |
91.9% |
2337964 |
62.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,096,084 |
G→A |
E160E (GAG→GAA) |
hisB → |
fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,096,084 | 0 | G | A | 100.0%
| 170.9
/ NA
| 49 | E160E (GAG→GAA) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/44); total (5/44) |
CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGT > W3110S.gb/2096045‑2096119
|
cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc > 1:1051159/1‑48 (MQ=255)
cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc > 1:112195/1‑48 (MQ=255)
cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc > 1:1182495/1‑48 (MQ=255)
cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc > 1:1522888/1‑48 (MQ=255)
cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCAc > 1:358224/1‑48 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2212605/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:952472/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2218298/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2267715/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2328722/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2347994/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2491046/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2519597/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2530293/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:320267/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:546119/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:606233/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:662127/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:729570/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:733405/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:73342/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:795866/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:798094/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:800926/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:912460/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1042981/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1646473/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1084864/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1232391/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:124534/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1298754/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1349040/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1494675/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1521851/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1645460/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2132676/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1648403/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1690434/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1735106/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1815868/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:1921915/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:200006/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2037208/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2075051/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2093380/65‑1 (MQ=255)
gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:2111381/65‑1 (MQ=255)
aaCCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:666951/63‑1 (MQ=255)
cTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:675167/59‑1 (MQ=255)
aCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt < 1:120317/55‑1 (MQ=255)
|
CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGT > W3110S.gb/2096045‑2096119
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
