Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F28 I1 R2 328 63.0 4687567 98.0% 4593815 65.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,096,084 G→A E160E (GAG→GAA)  hisB → fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,096,0840GA100.0% 387.1 / NA 128E160E (GAG→GAA) hisBfused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (69/59);  total (69/59)

Only 100 of 129 total aligned reads displayed.

GGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTC  >  W3110S.gb/2096018‑2096151
                                                                  |                                                                   
ggAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGaacaac                                                                 >  1:1537470/1‑71 (MQ=255)
   aaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCa                                                              >  1:1281386/1‑71 (MQ=255)
   aaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCa                                                              >  1:4628656/1‑71 (MQ=255)
        tGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGa                                                         <  1:1349819/71‑1 (MQ=255)
          ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACg                                                       >  1:3935466/1‑71 (MQ=255)
           gCATTACTGGTTTACGCTACGCCCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGt                                                      >  1:563190/1‑71 (MQ=255)
           gCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGt                                                      >  1:1155148/1‑71 (MQ=255)
           gCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGt                                                      >  1:4538004/1‑71 (MQ=255)
              ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAg                                                          <  1:248924/64‑1 (MQ=255)
              ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                                                   <  1:4018981/71‑1 (MQ=255)
                aatGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2220584/3‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACaa                                                                  >  1:2361036/1‑54 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAg                                                          >  1:3306786/1‑62 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGa                                                    >  1:1109946/1‑68 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGa                                                    >  1:3428747/1‑68 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:4572759/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:4567373/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:4391049/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:241880/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:790971/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2454718/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:4152401/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3080509/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2734403/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2823573/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:2889402/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1821513/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1220774/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1233807/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1083799/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3817616/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1427499/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:678984/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3499483/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3481853/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1583136/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:1590012/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:588463/1‑71 (MQ=255)
                aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg                                                 >  1:3400629/1‑71 (MQ=255)
                 cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGt                                                <  1:545225/71‑1 (MQ=255)
                 cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGt                                                >  1:3203632/1‑71 (MQ=255)
                 cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGt                                                <  1:3561114/71‑1 (MQ=255)
                    gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTAc                                             <  1:2500236/71‑1 (MQ=255)
                       tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCt                                          >  1:193526/1‑71 (MQ=255)
                       tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCt                                          >  1:1905523/1‑71 (MQ=255)
                       tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACACACCAGACGTGACCGTTACGCt                                          >  1:109534/1‑71 (MQ=255)
                        aCGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCt                                          <  1:3911605/70‑1 (MQ=255)
                        aCGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCt                                          <  1:1309032/70‑1 (MQ=255)
                         cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCa                                        <  1:3504639/71‑1 (MQ=255)
                         cGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCa                                        <  1:3740566/71‑1 (MQ=255)
                           cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg                                      <  1:167220/71‑1 (MQ=255)
                            tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg                                      >  1:1518713/1‑70 (MQ=255)
                             aCGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg                                      >  1:2874017/1‑69 (MQ=255)
                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:2714383/69‑1 (MQ=255)
                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:3265511/69‑1 (MQ=255)
                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:4571096/69‑1 (MQ=255)
                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:4631851/69‑1 (MQ=255)
                              cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:1716877/69‑1 (MQ=255)
                                   gcgAAACCCTGAACTTGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:3853340/71‑1 (MQ=255)
                                   gcgAAACCCTGAACTGGCCATTGATTGGCGAACAACTCTCCAGACGTGACCGTTACGCTCACGTTGTGCGt                              <  1:2559111/71‑1 (MQ=255)
                                   gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:1580315/71‑1 (MQ=255)
                                   gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:1171589/71‑1 (MQ=255)
                                   gcgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:715144/71‑1 (MQ=255)
                                    cgAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa                             <  1:1397169/71‑1 (MQ=255)
                                     gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                            <  1:1861660/71‑1 (MQ=255)
                                     gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa                             <  1:2981747/70‑1 (MQ=255)
                                              aagtGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa                     <  1:3855859/66‑1 (MQ=255)
                                              aaCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa                     <  1:382270/69‑1 (MQ=255)
                                              aaCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                   <  1:1700790/71‑1 (MQ=255)
                                              aaCTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                   <  1:662835/71‑1 (MQ=255)
                                                  ggCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg               >  1:1073105/1‑71 (MQ=255)
                                                   gCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg               >  1:4639623/1‑70 (MQ=255)
                                                    ccAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa             <  1:1049480/71‑1 (MQ=255)
                                                    ccAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa             <  1:2040209/71‑1 (MQ=255)
                                                    ccAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa             <  1:1021349/71‑1 (MQ=255)
                                                    ccAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa             <  1:364688/71‑1 (MQ=255)
                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:660148/43‑1 (MQ=255)
                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:4063174/43‑1 (MQ=255)
                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGt                                     <  1:412654/43‑1 (MQ=255)
                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTTCGTAATACCAAAGAGACGCAg            <  1:1730508/68‑1 (MQ=255)
                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                   <  1:148847/61‑1 (MQ=255)
                                                        tGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg            <  1:2230312/68‑1 (MQ=255)
                                                         gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAg                                   >  1:513599/1‑44 (MQ=255)
                                                         gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                          >  1:2455078/1‑53 (MQ=255)
                                                         gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt         >  1:3717087/1‑70 (MQ=255)
                                                          aTTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACg                                      >  1:3673375/1‑40 (MQ=255)
                                                          aTTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa       >  1:657697/1‑71 (MQ=255)
                                                          aTTGGCGAACAACTCACAAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa       >  1:2333351/1‑71 (MQ=255)
                                                           ttGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                            <  1:438522/49‑1 (MQ=255)
                                                           ttGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa       <  1:433754/70‑1 (MQ=255)
                                                             ggCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt                              <  1:3385689/45‑1 (MQ=255)
                                                             ggCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                          >  1:2079906/1‑49 (MQ=255)
                                                             ggCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                          >  1:4080427/1‑49 (MQ=255)
                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:1917572/70‑1 (MQ=255)
                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:1912150/70‑1 (MQ=255)
                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:1808104/70‑1 (MQ=255)
                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:3322708/70‑1 (MQ=255)
                                                              gCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:3595260/70‑1 (MQ=255)
                                                               cGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCg                               <  1:2429652/42‑1 (MQ=255)
                                                               cGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt    <  1:3505984/69‑1 (MQ=255)
                                                                  |                                                                   
GGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTC  >  W3110S.gb/2096018‑2096151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A