Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F28 I1 R1	354	41.7	3110465	93.1%	2895842	61.9

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,096,084	G→A	E160E (GAG→GAA)	hisB →	fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,096,084	0	G	A	97.2%	107.7 / ‑6.0	36	E160E (GAG→GAA)	hisB	fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (27/8); minor base C (1/0); total (28/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGT > W3110S.gb/2096038‑2096119

gTTTACGCTACGCCCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1572317/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGACCAACTCACCAGACGTGAc                   >  1:1320248/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACa                                   >  1:2999978/1‑49 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACGAGACGTGAc                   >  1:1413472/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:39995/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2401555/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2461209/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2976044/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:3010520/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:373294/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:385284/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1027593/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:402421/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:610742/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:639643/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:836678/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:879771/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:894762/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2255052/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1002320/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1331050/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1434053/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1486825/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1655985/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:1741674/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2096389/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2160703/1‑65 (MQ=255)

gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGAc                   >  1:2378752/1‑65 (MQ=255)

                 gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1822010/65‑1 (MQ=255)

                 gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2954696/65‑1 (MQ=255)

                 gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:405386/65‑1 (MQ=255)

                 gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1357682/65‑1 (MQ=255)

                 gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:2658624/65‑1 (MQ=255)

                 gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1305800/65‑1 (MQ=255)

                 gAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:944757/65‑1 (MQ=255)

                                   aTGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGt  <  1:1235653/47‑1 (MQ=255)

GTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGT > W3110S.gb/2096038‑2096119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A