Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R1
|
354 |
41.7 |
3110465 |
93.1% |
2895842 |
61.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,002,862 |
(T)7→8 |
intergenic (‑87/‑145) |
pitA ← / → yhiN |
phosphate transporter, low‑affinity/predicted oxidoreductase, FAD/NAD(P)‑binding domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,002,855 | 1 | . | T | 98.4%
| 211.3
/ ‑3.2
| 64 | intergenic (‑80/‑152) | pitA/yhiN | phosphate transporter, low‑affinity/predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (33/30); total (33/31) |
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
GCGGCGCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGAC‑TTTTTTTGATTTGCCCCCTGTATGGATTTCACTCAAAAAATAATTATCTTATATAATTCAGG > W3110S.gb/4002806‑4002917
|
gcggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGc < 1:36732/65‑1 (MQ=255)
cggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGcc > 1:957883/1‑65 (MQ=255)
cggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGcc > 1:2093991/1‑65 (MQ=255)
cggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGcc > 1:2303370/1‑65 (MQ=255)
cggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGcc > 1:2601735/1‑65 (MQ=255)
cggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGcc > 1:2629867/1‑65 (MQ=255)
cggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGcc > 1:1451344/1‑65 (MQ=255)
cggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGcc > 1:2853453/1‑65 (MQ=255)
cggcgCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGcc > 1:327112/1‑65 (MQ=255)
gcgcATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGccc < 1:1398835/64‑1 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGa > 1:1604940/1‑63 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2878960/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2801272/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:3069287/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2710193/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2603383/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:371463/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2470766/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:78773/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2286700/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:216187/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2272811/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2004833/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:1097067/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:149605/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:1422251/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:2004831/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGAt > 1:167616/1‑64 (MQ=255)
gTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCACTGTATGGAt > 1:2217872/1‑64 (MQ=38)
tCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTc > 1:190826/1‑64 (MQ=255)
tCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTc > 1:868540/1‑64 (MQ=255)
tCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTc > 1:115238/1‑64 (MQ=255)
tCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTc > 1:772732/1‑64 (MQ=255)
tCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTc > 1:612407/1‑64 (MQ=255)
tCGCGGCGCGGGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTc > 1:242400/1‑64 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1267490/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1284359/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:3012238/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:3010292/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1461377/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:316375/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1263290/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1262529/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1227072/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:638616/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:699465/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:728433/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1293656/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:2033339/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:2106233/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:215749/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1802592/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:2232660/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1778210/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:2398090/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1637006/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1566872/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:2625504/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:2664953/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:283997/65‑1 (MQ=255)
gcgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:2866091/65‑1 (MQ=255)
cgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:1735876/64‑1 (MQ=255)
cgcggGCAAAGTGAAATATAGACTTTTTTTTGATTTGCCCCCTGTATGGATTTCACTCaaaaaa < 1:2138511/64‑1 (MQ=255)
gAC‑TTTTTTTGATTTGCCCCCTGTATGGATTTCACTCAAAAAATAATTATCTTATATAATTCAgg < 1:1020121/65‑1 (MQ=255)
|
GCGGCGCATTATCAGTGACTTTCGCGGCGCGGGCAAAGTGAAATATAGAC‑TTTTTTTGATTTGCCCCCTGTATGGATTTCACTCAAAAAATAATTATCTTATATAATTCAGG > W3110S.gb/4002806‑4002917
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
