Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,000,806 |
A→T |
10.8% |
D37V (GAT→GTT) |
yhiO → |
predicted universal stress (ethanol tolerance) protein B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,000,806 | 0 | A | T | 10.8%
| 193.3
/ 15.3
| 74 | D37V (GAT→GTT) | yhiO | predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (29/37); new base T (3/5); total (32/42) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 |
GTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAG > W3110S.gb/4000736‑4000873
|
gTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGa > 1:1189894/1‑71 (MQ=255)
tGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2629820/70‑1 (MQ=255)
gCATTGTTAACATGGCACGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATcc < 1:2962847/70‑1 (MQ=255)
cATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCa < 1:2583235/70‑1 (MQ=255)
aTTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCAtt > 1:3457680/1‑71 (MQ=255)
tGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGc > 1:1533269/1‑71 (MQ=255)
gTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTg > 1:185037/1‑69 (MQ=255)
gTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGct > 1:3431963/1‑71 (MQ=255)
tAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGctc > 1:2155492/1‑70 (MQ=255)
cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:3674873/71‑1 (MQ=255)
aTGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCa < 1:1588999/71‑1 (MQ=255)
tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCaa < 1:538591/71‑1 (MQ=255)
tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCaa < 1:475929/71‑1 (MQ=255)
tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:2817700/71‑1 (MQ=255)
ggCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:1712035/56‑1 (MQ=255)
gcgcgcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:133810/1‑71 (MQ=255)
gcgcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATg > 1:3550778/1‑71 (MQ=255)
gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:900039/71‑1 (MQ=255)
gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:2280927/71‑1 (MQ=255)
tATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:916614/61‑1 (MQ=255)
aTTTCTCATCACTACGCTCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:2711565/68‑1 (MQ=255)
tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGa < 1:4427742/69‑1 (MQ=255)
tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2152787/47‑1 (MQ=255)
tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:2323103/47‑1 (MQ=255)
tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCa > 1:2843102/1‑49 (MQ=255)
tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:3538932/67‑1 (MQ=255)
tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:4433324/45‑1 (MQ=255)
tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCa > 1:1647834/1‑58 (MQ=255)
tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATgg < 1:2663065/70‑1 (MQ=255)
ctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCa > 1:3622941/1‑46 (MQ=255)
ctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:3507689/44‑1 (MQ=255)
tcatcaCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAgg > 1:4019583/1‑71 (MQ=255)
catcaCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAggg > 1:3970964/1‑71 (MQ=255)
catcaCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAggg > 1:564290/1‑71 (MQ=255)
tcaCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGtt < 1:1780861/60‑1 (MQ=255)
caCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGctct > 1:2636433/1‑48 (MQ=255)
caCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGctct > 1:1332314/1‑48 (MQ=255)
caCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:2232111/1‑55 (MQ=255)
cgcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCttttt < 1:503379/71‑1 (MQ=255)
gcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCtcaatcaa < 1:3011908/47‑1 (MQ=255)
ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCa < 1:171562/41‑1 (MQ=255)
ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTg < 1:4260379/49‑1 (MQ=255)
ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGAt < 1:4637766/51‑1 (MQ=255)
ttgttAGTGGTACTGCCTAACTGCGATCCATTGCTCTATCAATATGTTg < 1:2173471/49‑1 (MQ=255)
gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa > 1:652448/1‑40 (MQ=255)
gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:1436564/70‑1 (MQ=255)
gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:1100356/70‑1 (MQ=255)
ttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTcaca > 1:3808437/1‑71 (MQ=255)
ttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTcaca < 1:3865952/71‑1 (MQ=255)
tAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACAt > 1:911484/1‑71 (MQ=255)
aGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATg > 1:783584/1‑71 (MQ=255)
tGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:630274/41‑1 (MQ=255)
tGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCtttt < 1:870470/57‑1 (MQ=255)
gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGcc > 1:3571853/1‑70 (MQ=255)
tACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg < 1:69300/42‑1 (MQ=255)
tACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg < 1:2842267/42‑1 (MQ=255)
tACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa < 1:3493085/44‑1 (MQ=255)
aCTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATg < 1:3203083/41‑1 (MQ=255)
tGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:2044299/35‑1 (MQ=255)
gcttAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc > 1:3794236/4‑70 (MQ=255)
gCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACAt > 1:4255135/1‑61 (MQ=255)
gCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACAt > 1:2556298/1‑61 (MQ=255)
gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTa > 1:3074848/1‑53 (MQ=255)
gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTcaca > 1:3788907/1‑60 (MQ=255)
gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc > 1:1790591/1‑70 (MQ=255)
tAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCcaaca < 1:1636055/71‑1 (MQ=255)
tAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCcaaca < 1:2361224/71‑1 (MQ=255)
aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa < 1:2147253/71‑1 (MQ=255)
aCTGCGATCCATTGCTCTATCAATATGTTGATGGA‑GGGGCTTTTTTACCTCACATGGCCAACCCaacaaa < 1:1596514/70‑1 (MQ=255)
tGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:3693174/51‑1 (MQ=255)
gCGATCCATTGTTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAg > 1:1504994/1‑71 (MQ=255)
gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACAt > 1:1769385/1‑53 (MQ=255)
gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATgg > 1:1522919/1‑55 (MQ=255)
gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAg > 1:2364612/1‑71 (MQ=255)
cGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAg > 1:2344378/1‑70 (MQ=255)
aTCCATTGCTCTATCAATATGTTGATGGAGGGGGCtttttt < 1:3335448/41‑1 (MQ=255)
|
GTTTGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAG > W3110S.gb/4000736‑4000873
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
