Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F8 I0 R1 311 50.1 4085234 95.9% 3917739 60.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,000,806 A→T 25.1% D37V (GAT→GTT)  yhiO → predicted universal stress (ethanol tolerance) protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,000,8060AT25.1% 192.9 / 60.7 99D37V (GAT→GTT) yhiOpredicted universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base A (36/38);  new base T (11/14);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 8.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAGG  >  W3110S.gb/4000739‑4000874
                                                                   |                                                                    
tGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    >  1:617843/1‑70 (MQ=255)
    ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    <  1:879957/66‑1 (MQ=255)
    ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    <  1:1815977/66‑1 (MQ=255)
    ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    <  1:3765372/66‑1 (MQ=255)
    ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTg                                                               <  1:1305665/71‑1 (MQ=255)
    ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTg                                                               <  1:3984268/71‑1 (MQ=255)
     tGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    <  1:2705401/65‑1 (MQ=255)
      gTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGc                                                              <  1:1348249/70‑1 (MQ=255)
          aCATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTa                                                          <  1:193768/70‑1 (MQ=255)
          aCATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTa                                                          <  1:3636135/70‑1 (MQ=255)
           cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    <  1:2702977/59‑1 (MQ=255)
           cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    <  1:2043857/59‑1 (MQ=255)
           cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    <  1:3933474/59‑1 (MQ=255)
             tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa                                                      <  1:942895/71‑1 (MQ=255)
             tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa                                                      <  1:2240848/71‑1 (MQ=255)
             tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa                                                      <  1:1586883/71‑1 (MQ=255)
             tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa                                                      <  1:938011/71‑1 (MQ=255)
             tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa                                                      <  1:522099/71‑1 (MQ=255)
                  cgcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGt                                                 <  1:3173084/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:4027586/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:2652661/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:870938/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:3009816/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:3388663/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:3482692/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:3678658/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:1797994/71‑1 (MQ=255)
                   gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:3861725/71‑1 (MQ=255)
                      aTTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAata                                                    >  1:483458/1‑64 (MQ=255)
                      aTTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAata                                                    >  1:2019243/1‑64 (MQ=255)
                      aTTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAata                                                    >  1:1522126/1‑64 (MQ=255)
                      aTTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAata                                                    >  1:2442705/1‑64 (MQ=255)
                       tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc                                                                    <  1:1206434/47‑1 (MQ=255)
                       tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa                                                      >  1:2355887/1‑61 (MQ=255)
                       tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCa                                                       >  1:1312743/1‑60 (MQ=255)
                       tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGAAACAGCGATCCATTGCTGTATCaa                                                      >  1:2956751/1‑61 (MQ=255)
                         tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATgg                                           <  1:570792/70‑1 (MQ=255)
                         tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATg                                            >  1:517364/1‑69 (MQ=255)
                         tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata                                                    >  1:161500/1‑61 (MQ=255)
                         tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata                                                    >  1:796744/1‑61 (MQ=255)
                         tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg                                            >  1:810412/1‑69 (MQ=255)
                         tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg                                            >  1:254284/1‑69 (MQ=255)
                         tctcATCACTACGCGCGTTGTTAGTGGTACTGAGTAACTGCGATCCa                                                                  >  1:2508717/1‑47 (MQ=255)
                         tctcATAACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATGCATTGCTCTATCAATATGTTGa                                              >  1:120761/1‑67 (MQ=255)
                         tctaATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata                                                    >  1:2924890/1‑61 (MQ=255)
                                aCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAatat                                                   >  1:3070683/1‑55 (MQ=255)
                                aCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCt                                   >  1:265829/1‑71 (MQ=255)
                                aCTACGCGCGTTGTTAGTGCTACTGCGTAACTTCGATCAATTGCTCTATCAATATGTTGATGGAGGGGGCt                                   >  1:1908694/1‑71 (MQ=255)
                                 cTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCtt                                  <  1:2399521/71‑1 (MQ=255)
                                    cgcgcgTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGa                                              >  1:679244/1‑56 (MQ=255)
                                    cgcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCttttt                               <  1:2845652/71‑1 (MQ=255)
                                     gcgcgTTGTTAGTGGTACTGCTTAACTGCGTTCCATTGCTCTATCAATATCTTGATgg                                           <  1:1085005/58‑1 (MQ=255)
                                     gcgcgTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAGTGGGCtttttt                              <  1:82225/71‑1 (MQ=255)
                                        cgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTAcc                           >  1:1880675/1‑71 (MQ=255)
                                          ttgttAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGtt                                                <  1:1277415/48‑1 (MQ=255)
                                          ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc                                                        <  1:719088/40‑1 (MQ=255)
                                          ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa                                                      <  1:2586694/42‑1 (MQ=255)
                                          ttgttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa                                                      <  1:3935691/42‑1 (MQ=255)
                                            gttAGTGGTACTGGGTAACTGCGATCCATTGCTCTATCaa                                                      >  1:406734/1‑40 (MQ=255)
                                            gttAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATTTGTTGATGGAGGGGGCTTTTTTACCTca                        <  1:2068920/70‑1 (MQ=255)
                                            gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTa                                                          >  1:885548/1‑36 (MQ=255)
                                               aGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa                                          >  1:2518884/1‑49 (MQ=255)
                                               aGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa                                          >  1:438944/1‑49 (MQ=255)
                                                   gTACTGCGTAACTGCGTTCCATTGCTCTATCAata                                                    >  1:1889268/1‑35 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATatgatg                                               >  1:3092303/1‑40 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:63522/39‑1 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa                                              >  1:442966/1‑41 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa                                              >  1:517129/1‑41 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa                                              >  1:1485983/1‑41 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa                                              >  1:3863668/1‑41 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa                                              >  1:1849945/1‑41 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa                                              >  1:1906453/1‑41 (MQ=255)
                                                   gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa                                              >  1:3161903/1‑41 (MQ=255)
                                                    tACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:1522073/38‑1 (MQ=255)
                                                    tACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt                                                <  1:280762/38‑1 (MQ=255)
                                                     aCTCCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTc                         <  1:518029/60‑1 (MQ=255)
                                                       tGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGa                                          <  1:1620821/41‑1 (MQ=255)
                                                       tGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGa                                          <  1:3342013/41‑1 (MQ=255)
                                                       tGCGTAACTCCGTTCCATTGCTCTATCAATATGTTGATGGa                                          <  1:473505/41‑1 (MQ=38)
                                                        gCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAgg                                        >  1:1884274/1‑42 (MQ=255)
                                                        gCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGc                  >  1:2920148/1‑64 (MQ=255)
                                                        gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGTGGCTTTTTTACCTCACATGGccaaccc            >  1:2716216/1‑70 (MQ=255)
                                                         cGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc            <  1:3302194/69‑1 (MQ=255)
                                                         cGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc            <  1:3149042/69‑1 (MQ=255)
                                                         cGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACATCACAAGGccaacccaa          >  1:1068040/1‑71 (MQ=255)
                                                             aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCcaacaa       <  1:1536399/70‑1 (MQ=255)
                                                             aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa      >  1:365210/1‑71 (MQ=255)
                                                             aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa      >  1:3023654/1‑71 (MQ=255)
                                                             aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa      >  1:1005685/1‑71 (MQ=255)
                                                              cTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca                        <  1:643502/52‑1 (MQ=255)
                                                              cTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca                        <  1:3952765/52‑1 (MQ=255)
                                                              cTGCGTTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca                        <  1:3801662/52‑1 (MQ=255)
                                                                gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCt                          >  1:165484/1‑48 (MQ=255)
                                                                gCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCt                          >  1:2781186/1‑48 (MQ=255)
                                                                gCGATCCATTGCTCTATCAATATGTTGATGGAGCGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAg   >  1:3489323/1‑71 (MQ=255)
                                                                 cGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAgg  >  1:1446070/1‑71 (MQ=255)
                                                                 cGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAgg  >  1:302611/1‑71 (MQ=255)
                                                                   aTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca                        <  1:1581116/47‑1 (MQ=255)
                                                                   aTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca                        <  1:733633/47‑1 (MQ=255)
                                                                   aTCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca                        <  1:1909013/47‑1 (MQ=255)
                                                                   |                                                                    
TGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAACAGG  >  W3110S.gb/4000739‑4000874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A