Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,000,806 |
A→T |
7.8% |
D37V (GAT→GTT) |
yhiO → |
predicted universal stress (ethanol tolerance) protein B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,000,806 | 0 | A | T | 7.8%
| 137.7
/ 4.3
| 51 | D37V (GAT→GTT) | yhiO | predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (18/29); new base T (2/2); total (20/31) |
| Fisher's exact test for biased strand distribution p-value = 6.40e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 |
TGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAA > W3110S.gb/4000739‑4000870
|
tGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc > 1:1875032/1‑70 (MQ=255)
tGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATc < 1:1315702/70‑1 (MQ=255)
gCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATcc < 1:1263246/70‑1 (MQ=255)
cATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCAt < 1:2130704/71‑1 (MQ=255)
aTTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCAtt > 1:2062866/1‑71 (MQ=255)
ttGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTc < 1:2788558/66‑1 (MQ=255)
cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:159388/71‑1 (MQ=255)
cATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc < 1:484233/71‑1 (MQ=255)
aTGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATc > 1:2386371/1‑70 (MQ=255)
tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:706107/71‑1 (MQ=255)
tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:884052/71‑1 (MQ=255)
tGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:927035/71‑1 (MQ=255)
gcgcgcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAata > 1:2688235/1‑71 (MQ=255)
gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:1664218/71‑1 (MQ=255)
gcTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:1975668/71‑1 (MQ=255)
cTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:679075/70‑1 (MQ=255)
tATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTg < 1:2200052/70‑1 (MQ=255)
tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:67230/61‑1 (MQ=255)
tttCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCaa < 1:1741403/61‑1 (MQ=255)
tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTg > 1:2630857/1‑50 (MQ=255)
tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAATATGTTGATg > 1:191405/1‑69 (MQ=255)
tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATgg < 1:918734/70‑1 (MQ=255)
tctcATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg > 1:1716771/1‑69 (MQ=255)
aCTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCt > 1:1681067/1‑71 (MQ=255)
cTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCtt < 1:1249368/71‑1 (MQ=255)
tACGCGCGTTGTTAGTGGTACTGCGTAACTGCGTTCCATTGCTCTATCAat < 1:383643/51‑1 (MQ=255)
tACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAatat < 1:2798599/53‑1 (MQ=255)
tACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATg < 1:755496/60‑1 (MQ=255)
cgcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCAt > 1:1649489/1‑37 (MQ=255)
cgcgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTCCTCTATCAATATGTTGATGGAGGGGGCttttt > 1:2803839/1‑71 (MQ=255)
cgcgTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:405410/52‑1 (MQ=255)
ttgttAGTGGTACTGCGTAACTGCGATCCATTGctc < 1:1079988/36‑1 (MQ=255)
ttgttAGTGGTACTGCGTAACTGCGATCCATTGctc < 1:583481/36‑1 (MQ=255)
gttAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTca < 1:995583/70‑1 (MQ=255)
tGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGtt < 1:1174885/41‑1 (MQ=255)
gTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGa > 1:2688404/1‑41 (MQ=255)
aCTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa > 1:1818551/1‑43 (MQ=255)
aCTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa > 1:538026/1‑43 (MQ=255)
aCTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGa > 1:1500247/1‑43 (MQ=255)
cTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTAc < 1:1136115/56‑1 (MQ=255)
cTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTAc < 1:974372/56‑1 (MQ=255)
cTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACATCACATGGccaacc > 1:1529245/1‑71 (MQ=255)
tGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATgg < 1:1678012/40‑1 (MQ=255)
tGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATgg < 1:978650/40‑1 (MQ=255)
gCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAg > 1:55252/1‑41 (MQ=255)
cGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaacccaa > 1:1203351/1‑71 (MQ=255)
gTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGccaaccc < 1:341069/68‑1 (MQ=255)
aCTGCGATCCATTGCTCTATCAATATGTTGATGGAg > 1:1010480/1‑36 (MQ=255)
aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGTCTTTTTTACCTCACATGGCCAACCcaacaa > 1:1569033/1‑70 (MQ=255)
aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa < 1:2545317/71‑1 (MQ=255)
aCTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCaacaaa > 1:946721/1‑71 (MQ=255)
|
TGCATTGTTAACATGGCGCGCTATTTCTCATCACTACGCGCGTTGTTAGTGGTACTGCGTAACTGCGATCCATTGCTCTATCAATATGTTGATGGAGGGGGCTTTTTTACCTCACATGGCCAACCCAACAAA > W3110S.gb/4000739‑4000870
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
