Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R2
|
308 |
27.9 |
1975068 |
96.7% |
1909890 |
67.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,301,561 |
G→A |
24.5% |
D429D (GAC→GAT) |
fdhF ← |
formate dehydrogenase‑H, selenopolypeptide subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,301,561 | 0 | G | A | 24.5%
| 93.1
/ 28.7
| 49 | D429D (GAC→GAT) | fdhF | formate dehydrogenase‑H, selenopolypeptide subunit |
| Reads supporting (aligned to +/- strand): ref base G (12/25); new base A (2/10); total (14/35) |
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 |
CATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTG > W3110S.gb/4301494‑4301629
|
cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:1970494/1‑71 (MQ=255)
cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:227414/1‑71 (MQ=255)
cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:1327687/1‑71 (MQ=255)
cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:1337641/1‑71 (MQ=255)
cATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTcc > 1:571442/1‑71 (MQ=255)
tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:412802/71‑1 (MQ=255)
tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:1688316/71‑1 (MQ=255)
tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:1185168/71‑1 (MQ=255)
tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:1246938/71‑1 (MQ=255)
tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:825698/71‑1 (MQ=255)
tGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTg < 1:1368213/71‑1 (MQ=255)
gCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAAc < 1:1843014/69‑1 (MQ=255)
ttGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAcc < 1:1366027/65‑1 (MQ=255)
ttGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAcc < 1:1812299/65‑1 (MQ=255)
ttGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAcc < 1:1961421/65‑1 (MQ=255)
ttGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAcc < 1:58832/65‑1 (MQ=255)
gACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTc > 1:1919853/1‑68 (MQ=255)
gACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTc > 1:1387296/1‑68 (MQ=255)
gACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTc > 1:1175911/1‑68 (MQ=255)
gACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTc > 1:116864/1‑68 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAg < 1:653263/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAg < 1:849737/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:390978/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1899036/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:484194/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:168768/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:532422/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1532908/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1530413/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1489510/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAg < 1:1137450/70‑1 (MQ=255)
aCGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTACCAg < 1:1124943/70‑1 (MQ=255)
aaTAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAAc < 1:1601519/54‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:851219/70‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:396958/70‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:1017355/70‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:262435/70‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:1857017/70‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:1295052/70‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:734631/70‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:1624141/70‑1 (MQ=255)
cgccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTa < 1:586034/70‑1 (MQ=255)
gccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACg < 1:76551/71‑1 (MQ=255)
gccgccgACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACg < 1:547938/71‑1 (MQ=255)
gcgGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGa > 1:980315/1‑71 (MQ=255)
gcgGTTTTGGTCATAAAGATATCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGa > 1:524719/1‑71 (MQ=255)
aaaGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCa > 1:566071/1‑70 (MQ=255)
aaaGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCa > 1:412557/1‑70 (MQ=255)
aaaGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAg > 1:357872/1‑71 (MQ=255)
gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:648759/1‑69 (MQ=255)
gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:1394097/1‑69 (MQ=255)
gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:1651206/1‑69 (MQ=255)
gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:32208/1‑69 (MQ=255)
gTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTg > 1:971224/1‑69 (MQ=255)
|
CATGCTCGCCCCACGACGTTGACGGTAAAATAACATCCGCCGCCGACGCGGTTTTGGTCATAAAGATGTCCTGAACGATAACCAGTTCCAGATCTTCAAAGGCTTTACGTACTGCCGACAGCTCCGCGTCAGTTTG > W3110S.gb/4301494‑4301629
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
