Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
848,718 |
A→G |
34.8% |
intergenic (‑292/+112) |
glnH ← / ← dps |
glutamine transporter subunit/Fe‑binding and storage protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 848,718 | 0 | A | G | 34.8%
| 43.8
/ 44.6
| 46 | intergenic (‑292/+112) | glnH/dps | glutamine transporter subunit/Fe‑binding and storage protein |
| Reads supporting (aligned to +/- strand): ref base A (18/12); new base G (3/13); total (21/25) |
| Fisher's exact test for biased strand distribution p-value = 1.22e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGG > W3110S.gb/848648‑848782
|
aaaaaaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATa < 1:1229429/71‑1 (MQ=255)
aaaaaaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATa < 1:1528899/71‑1 (MQ=255)
aaaaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAg > 1:94539/1‑70 (MQ=255)
aaaaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAg > 1:859678/1‑70 (MQ=255)
aaaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATGGCa < 1:3354370/71‑1 (MQ=255)
aaaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCa < 1:991633/71‑1 (MQ=255)
aaCAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTGCTTAATCAGCTGGAGATAg > 1:994413/1‑68 (MQ=255)
aaTCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATGGCAgatg < 1:2340925/71‑1 (MQ=255)
aaTCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATGGCAgatg < 1:3604896/71‑1 (MQ=255)
aaTCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAgatg < 1:651370/71‑1 (MQ=255)
aaTCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAgatg < 1:369037/71‑1 (MQ=255)
tATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATGGCAgatggatg < 1:950703/69‑1 (MQ=255)
tATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATGGCAgatggatg < 1:4075753/69‑1 (MQ=255)
tAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTa < 1:3623670/70‑1 (MQ=255)
aCGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTaaa > 1:1221529/1‑70 (MQ=255)
cGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATa < 1:1894289/71‑1 (MQ=255)
ttGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATaa > 1:379426/1‑69 (MQ=255)
gtgtgATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCAc > 1:926684/1‑60 (MQ=255)
gtgtgATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCAc > 1:1953947/1‑60 (MQ=255)
gtgtgATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCAc > 1:3381378/1‑60 (MQ=255)
gatgatGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCa > 1:3242227/1‑55 (MQ=255)
atgTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGt > 1:3799589/1‑71 (MQ=255)
atgTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGt > 1:3518746/1‑71 (MQ=255)
tgtgGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATa < 1:2721962/57‑1 (MQ=255)
tgGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGa < 1:2874087/65‑1 (MQ=255)
aTACAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTa < 1:2485291/48‑1 (MQ=255)
aTACAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATa < 1:2054481/52‑1 (MQ=255)
aTACAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATa < 1:2308294/52‑1 (MQ=255)
aTACAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATa < 1:3817955/52‑1 (MQ=255)
aTACAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATa < 1:2433148/52‑1 (MQ=255)
aTACAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATa < 1:2692811/52‑1 (MQ=255)
aTACAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATa < 1:2965051/52‑1 (MQ=255)
aTACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAAt < 1:1231117/51‑1 (MQ=255)
aCAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTaaa > 1:3465503/1‑48 (MQ=255)
aCAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGc > 1:2667990/1‑70 (MQ=255)
aCAAAAAATTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGc > 1:776016/1‑70 (MQ=255)
aCAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGc > 1:2329901/1‑70 (MQ=255)
cAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGc < 1:2023943/54‑1 (MQ=255)
aaaaaTTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGc < 1:3375313/52‑1 (MQ=255)
aTTTACTTAATCAGCTGGAGATGGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGcaaaacaa < 1:3892676/70‑1 (MQ=255)
aaTCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAgg > 1:458570/1‑47 (MQ=255)
aaTCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAgg > 1:2320930/1‑47 (MQ=255)
aGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGt > 1:3778322/1‑38 (MQ=255)
aGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGt > 1:4046813/1‑38 (MQ=255)
aGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGt > 1:2528277/1‑38 (MQ=255)
gCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTc < 1:6310/71‑1 (MQ=255)
gCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTc < 1:3819823/71‑1 (MQ=255)
agaTAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAgg > 1:2628604/1‑69 (MQ=255)
|
AAAAAACAATCTTTATGTAACGATTGTGTGATGATGTGGATACAAAAAATTTACTTAATCAGCTGGAGATAGCAGATGGATGCACTAAATAAGTGCGTTGAGGTGGGCTGCAAAACAAAACGGCCTCCTGTCAGG > W3110S.gb/848648‑848782
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
