Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R1	14	46.8	1456587	99.0%	1442021	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,369,969	T→A	T128S (ACC→TCC)	nanK ←	N‑acetylmannosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,369,969	0	T	A	100.0%	205.1 / NA	52	T128S (ACC→TCC)	nanK	N‑acetylmannosamine kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (22/30); total (22/30)

ACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATT  >  NC_000913/3369835‑3370116

aCCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCg                                                                                                                                     <  1:283302/151‑1 (MQ=255)

  cTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGt                                                                                                                                   >  1:330828/1‑151 (MQ=255)

        gTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATc                                                                                                                             <  1:458891/151‑1 (MQ=255)

        gTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATc                                                                                                                             >  2:283583/1‑151 (MQ=255)

        gTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATc                                                                                                                             >  2:276230/1‑151 (MQ=255)

          ccACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACGCCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTtata                                                                                                                               <  2:152818/147‑1 (MQ=255)

          ccACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGc                                                                                                                           <  1:229329/151‑1 (MQ=255)

            acaGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGcc                                                                                                                          >  1:522024/1‑150 (MQ=255)

              aGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATc                                                                                                                       <  1:432642/151‑1 (MQ=255)

                ccGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCa                                                                                                                     <  1:373251/151‑1 (MQ=255)

                       cTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTg                                                                                                              >  2:409870/1‑151 (MQ=255)

                              gtgtGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTcc                                                                                                       >  1:17837/1‑151 (MQ=255)

                              gtgtGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTcc                                                                                                       >  1:395601/1‑151 (MQ=255)

                              gtgtGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTcc                                                                                                       <  1:594433/151‑1 (MQ=255)

                               tgtgGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGGTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTcc                                                                                                       >  2:194735/1‑150 (MQ=255)

                                  ggATCGGCAAGCGTATGCCCGATATCCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGccc                                                                                                   <  2:281167/151‑1 (MQ=255)

                                   gATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGccc                                                                                                   >  1:139597/1‑150 (MQ=255)

                                          aaGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCg                                                                                           <  1:144205/151‑1 (MQ=255)

                                              gTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCt                                                                                       <  1:44252/151‑1 (MQ=255)

                                                 tGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTgcg                                                                                    >  2:606196/1‑151 (MQ=255)

                                                   cccGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTgcgc                                                                                   <  2:286118/150‑1 (MQ=255)

                                                    ccGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGcgt                                                                                 <  1:210906/151‑1 (MQ=255)

                                                     cGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGcgtc                                                                                <  1:265946/151‑1 (MQ=255)

                                                      gATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGcgtcg                                                                               >  2:258695/1‑151 (MQ=255)

                                                        tatGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGtt                                                                             >  2:533936/1‑151 (MQ=255)

                                                         atGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGATCACAGCACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCTCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGtt                                                                             <  2:544514/150‑1 (MQ=255)

                                                            ccccGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTaa                                                                           <  2:632557/149‑1 (MQ=255)

                                                                 ccAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAAt                                                                    <  2:295659/151‑1 (MQ=255)

                                                                           aGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCaa                                                           >  2:20761/1‑150 (MQ=255)

                                                                            gggCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAAt                                                         <  1:395679/151‑1 (MQ=255)

                                                                                  gTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTggca                                                    >  2:164292/1‑148 (MQ=255)

                                                                                   tAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTaa                                                   <  2:650152/150‑1 (MQ=255)

                                                                                        aGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttgtt                                             <  1:21504/151‑1 (MQ=255)

                                                                                            tGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCa                                          <  2:521982/150‑1 (MQ=255)

                                                                                                  cGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttgtt                                             <  2:109987/141‑1 (MQ=255)

                                                                                                      cACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGAc                                <  2:563894/150‑1 (MQ=255)

                                                                                                      cACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACt                               >  2:269029/1‑151 (MQ=255)

                                                                                                        cTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTa                              <  2:305275/150‑1 (MQ=255)

                                                                                                          acacCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAAc                            <  1:563418/150‑1 (MQ=255)

                                                                                                             ccgccgCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGa                         <  1:563229/150‑1 (MQ=255)

                                                                                                                   ccAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTa                  <  2:304206/151‑1 (MQ=255)

                                                                                                                    cAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAg                 <  2:306754/151‑1 (MQ=255)

                                                                                                                     aaCGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGc                >  2:479239/1‑151 (MQ=255)

                                                                                                                     aaCGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGc                <  2:616310/151‑1 (MQ=255)

                                                                                                                      aCGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCa               <  2:421158/151‑1 (MQ=255)

                                                                                                                        gCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAAt             >  1:501499/1‑151 (MQ=255)

                                                                                                                        gCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAAt             >  1:119563/1‑151 (MQ=255)

                                                                                                                         ccGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATc            >  1:167404/1‑151 (MQ=255)

                                                                                                                               gAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCaa      <  2:372222/151‑1 (MQ=255)

                                                                                                                                 aaCGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccacca       >  2:90487/1‑148 (MQ=255)

                                                                                                                                  aCGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccacca       >  2:253388/1‑147 (MQ=255)

                                                                                                                                   cGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGAtt  >  1:433407/1‑151 (MQ=255)

ACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACCAAGATT  >  NC_000913/3369835‑3370116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A