Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I2 R1	14	50.4	1586623	98.1%	1556477	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,369,969	T→A	T128S (ACC→TCC)	nanK ←	N‑acetylmannosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,369,969	0	T	A	100.0%	190.2 / NA	49	T128S (ACC→TCC)	nanK	N‑acetylmannosamine kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (17/32); total (17/32)

TTCCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACC  >  NC_000913/3369826‑3370110

ttCCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATaa                                                                                                                                          >  1:334998/1‑149 (MQ=255)

ttCCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATaa                                                                                                                                          >  1:62943/1‑149 (MQ=255)

  ccACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGAc                                                                                                                                      >  1:241428/1‑151 (MQ=255)

     cGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCa                                                                                                                                    <  2:128998/150‑1 (MQ=255)

       cAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCatat                                                                                                                                 <  1:90961/151‑1 (MQ=255)

       cAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCata                                                                                                                                  <  2:274417/150‑1 (MQ=255)

          ccTGTGGGCCCACAGCCGCTGCCTGGGCCGTGTGGATTGGCAAGCGTATGCCCGCTATGCCCCGCCAGACCGCCAGGGCAGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATACAGACCATAGCg                                                                                                                               <  2:646244/150‑1 (MQ=255)

            tgtgCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGtt                                                                                                                            >  1:756839/1‑151 (MQ=255)

             gtgCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATCCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGtt                                                                                                                            <  2:203478/150‑1 (MQ=255)

                   ccACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCg                                                                                                                      <  1:45417/150‑1 (MQ=255)

                      caGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCa                                                                                                                   <  2:661042/150‑1 (MQ=255)

                      caGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCAt                                                                                                                  >  2:534561/1‑151 (MQ=255)

                          cGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCCCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAg                                                                                                              <  2:404319/151‑1 (MQ=255)

                           gCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAgc                                                                                                             <  1:205284/151‑1 (MQ=255)

                            cAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAgc                                                                                                             >  2:581714/1‑150 (MQ=255)

                             aGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAgcgc                                                                                                           >  2:295677/1‑151 (MQ=255)

                                   ggCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGaa                                                                                                      >  1:397876/1‑150 (MQ=255)

                                       gtgtGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTcc                                                                                                 >  1:683625/1‑151 (MQ=255)

                                               cGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCAtg                                                                                          <  2:341399/150‑1 (MQ=255)

                                               cGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCAtg                                                                                          <  1:412282/150‑1 (MQ=255)

                                                   aaGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCg                                                                                     >  2:712428/1‑151 (MQ=255)

                                                     gctgaTGCCCGTGATGCCCGGCGAGACCGCCAGGGCCGGTAAGAAGTTTGCAGCGCCTCGCTACACCGCCGACAAGGTCGGTGGAAACGGAGAGAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAGCTCCGCCCAtgctgc                                                                                      <  2:624629/144‑1 (MQ=255)

                                                     gCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGc                                                                                   >  2:599417/1‑151 (MQ=255)

                                                         aTGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTgc                                                                               <  2:125411/151‑1 (MQ=255)

                                                             ccGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGcgt                                                                           <  2:201811/151‑1 (MQ=255)

                                                                  atGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGtt                                                                       <  2:184617/150‑1 (MQ=255)

                                                                  atGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGtt                                                                       >  1:404607/1‑150 (MQ=255)

                                                                     ccccGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATg                                                                   <  1:534626/151‑1 (MQ=255)

                                                                          ccAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCa                                                                <  2:424259/149‑1 (MQ=255)

                                                                          ccAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAAt                                                              <  1:431966/151‑1 (MQ=255)

                                                                          ccAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAAt                                                              <  1:621300/151‑1 (MQ=255)

                                                                            aGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAACCTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATgg                                                            <  2:551344/151‑1 (MQ=255)

                                                                            aGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATgg                                                            <  2:404550/151‑1 (MQ=255)

                                                                             gACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGt                                                           <  1:281576/151‑1 (MQ=255)

                                                                                  ccAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGc                                                       >  1:406485/1‑150 (MQ=255)

                                                                                  ccAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCGGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCa                                                      <  2:500399/151‑1 (MQ=255)

                                                                                   cAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCaa                                                     <  1:759096/151‑1 (MQ=255)

                                                                                          ggTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTa                                              <  2:401794/151‑1 (MQ=255)

                                                                                               gCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttg                                         >  2:519154/1‑151 (MQ=255)

                                                                                                   tttGCAGCCTCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCAGGCTGCGTCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttgt                                        <  2:559622/148‑1 (MQ=255)

                                                                                                     tGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTcc                                     >  2:460838/1‑149 (MQ=255)

                                                                                                       cAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCg                                 <  1:462060/151‑1 (MQ=255)

                                                                                                        aGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGt                                >  1:215225/1‑151 (MQ=255)

                                                                                                             cTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTg                            <  2:180995/150‑1 (MQ=255)

                                                                                                                   acacCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAAc                      <  1:401144/150‑1 (MQ=255)

                                                                                                                      ccgccgCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACgg                    <  2:453950/149‑1 (MQ=255)

                                                                                                                                    ggTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATcca    <  2:756740/151‑1 (MQ=255)

                                                                                                                                     gTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccac   <  1:309885/151‑1 (MQ=255)

                                                                                                                                       ggAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccacc  >  2:586260/1‑150 (MQ=255)

TTCCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACC  >  NC_000913/3369826‑3370110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A