Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,376,341	+G	coding (436/498 nt)	sspB ←	ClpXP protease specificity enhancing factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,376,339	1	.	G	100.0%	148.3 / NA	40	P146P (CCT→CCC)	sspB	ClpXP protease specificity enhancing factor
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (20/20); total (20/20)

GTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑GGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCC  >  NC_000913/3376189‑3376482

gTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCa                                                                                                                                                  <  2:596231/151‑1 (MQ=255)

  cGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑gg                                                                                                                                               >  1:740008/1‑151 (MQ=255)

   ggTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑ggg                                                                                                                                              >  1:806575/1‑150 (MQ=255)

           ggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAgtgt                                                                                                                                       >  2:804944/1‑151 (MQ=255)

                  atGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCatcatc                                                                                                                                >  2:812894/1‑151 (MQ=255)

                    gATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCg                                                                                                                               >  2:495923/1‑150 (MQ=255)

                       tGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGa                                                                                                                            >  2:661865/1‑150 (MQ=255)

                        gCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGa                                                                                                                            <  1:721911/149‑1 (MQ=255)

                        gCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGAt                                                                                                                           <  2:636530/150‑1 (MQ=255)

                                cACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGt                                                                                                                  <  2:610996/151‑1 (MQ=255)

                                      aTTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCAt                                                                                                            >  2:374326/1‑151 (MQ=255)

                                        tttATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATc                                                                                                           >  1:454667/1‑150 (MQ=255)

                                         ttattaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCa                                                                                                          >  2:650899/1‑150 (MQ=255)

                                         ttattaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCa                                                                                                          <  1:495887/150‑1 (MQ=255)

                                           attaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAAt                                                                                                        >  1:790180/1‑150 (MQ=255)

                                           attaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATa                                                                                                       <  2:9076/151‑1 (MQ=255)

                                                   aTTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACAt                                                                                               >  1:638911/1‑151 (MQ=255)

                                                      aaaaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAAc                                                                                            >  1:687152/1‑151 (MQ=255)

                                                      aaaaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAAc                                                                                            <  1:56905/151‑1 (MQ=255)

                                                        aaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACgg                                                                                          >  2:100012/1‑151 (MQ=255)

                                                        aaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACgg                                                                                          <  1:130429/151‑1 (MQ=255)

                                                              aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGt                                                                                    >  2:145765/1‑151 (MQ=255)

                                                                 aCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGt                                                                                 >  2:362603/1‑151 (MQ=255)

                                                                    ggCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTg                                                                              <  1:185817/151‑1 (MQ=255)

                                                                       cgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGGGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGGGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCg                                                                           <  2:745814/151‑1 (MQ=255)

                                                                            gggCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATg                                                                        <  1:804888/149‑1 (MQ=255)

                                                                                 tGTTTTGTATTACTTCACAACGCTTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCctct                                                                   <  1:684006/149‑1 (MQ=255)

                                                                                         aTTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCAtcattca                                                         <  2:122052/151‑1 (MQ=255)

                                                                                                  caACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTgg                                                >  2:105040/1‑151 (MQ=255)

                                                                                                  caACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTgg                                                >  2:819792/1‑151 (MQ=255)

                                                                                                     cgcgTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTAt                                             <  2:11486/151‑1 (MQ=255)

                                                                                                          aaTGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTc                                         <  2:370912/150‑1 (MQ=255)

                                                                                                           aTGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCAt                                       >  2:207421/1‑151 (MQ=255)

                                                                                                           aTGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCAt                                       <  1:339814/151‑1 (MQ=255)

                                                                                                                ggTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAg                                  <  2:843350/151‑1 (MQ=255)

                                                                                                                   cGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGc                                >  1:751792/1‑150 (MQ=255)

                                                                                                                   cGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGc                                <  1:455140/150‑1 (MQ=255)

                                                                                                                    gACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGc                                >  1:547796/1‑149 (MQ=255)

                                                                                                                         ccGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCa                         <  1:730758/151‑1 (MQ=255)

                                                                                                                               ggtggCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCa                   <  1:130209/151‑1 (MQ=255)

                                                                                                                                   gCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAAc                >  1:466912/1‑150 (MQ=255)

                                                                                                                                            gTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTg      >  1:240734/1‑151 (MQ=255)

                                                                                                                                                 tCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGcc  <  2:88058/150‑1 (MQ=255)

GTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑GGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCC  >  NC_000913/3376189‑3376482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A