Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,376,341	+G	coding (436/498 nt)	sspB ←	ClpXP protease specificity enhancing factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,376,339	1	.	G	100.0%	159.9 / NA	42	P146P (CCT→CCC)	sspB	ClpXP protease specificity enhancing factor
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (25/17); total (25/17)

GGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑GGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCC  >  NC_000913/3376192‑3376482

ggTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑ggg                                                                                                                                              <  1:433916/151‑2 (MQ=255)

      gtggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAgt                                                                                                                                         >  1:133663/1‑151 (MQ=255)

              tatGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTcatcat                                                                                                                                 >  2:721315/1‑151 (MQ=255)

               atGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCatcatc                                                                                                                                >  1:462999/1‑151 (MQ=255)

                 gATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCg                                                                                                                               <  2:365970/150‑1 (MQ=255)

                    tGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGa                                                                                                                            <  1:354761/150‑1 (MQ=255)

                     gCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATc                                                                                                                          >  1:343529/1‑151 (MQ=255)

                       cacaATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCt                                                                                                                         >  2:445194/1‑150 (MQ=255)

                          aaTCACGCTATTTTATTAAGTCATCAAAAAGACAAAACAGGCCGCCTCGCCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCCCCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGCTGAGTGTCATCATCGTGATCTGGCt                                                                                                                     >  1:711338/1‑151 (MQ=255)

                            tCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTg                                                                                                                   >  1:691233/1‑151 (MQ=255)

                               cGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCg                                                                                                                >  2:476535/1‑151 (MQ=255)

                                gCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGc                                                                                                               >  2:592682/1‑151 (MQ=255)

                                      ttattaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCa                                                                                                          >  1:110886/1‑150 (MQ=255)

                                      ttattaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCa                                                                                                          >  2:402145/1‑150 (MQ=255)

                                          taAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATaa                                                                                                      <  2:456220/150‑1 (MQ=255)

                                           aaGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAcc                                                                                                    <  1:530085/151‑1 (MQ=255)

                                               cATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGAc                                                                                                 >  1:10978/1‑150 (MQ=255)

                                               cATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGAc                                                                                                 <  2:630393/150‑1 (MQ=255)

                                               cATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGAc                                                                                                 >  1:494280/1‑150 (MQ=255)

                                                    aaaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACg                                                                                           >  1:190467/1‑151 (MQ=255)

                                                         aCAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTc                                                                                      >  1:536414/1‑151 (MQ=255)

                                                               cAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGt                                                                                 >  2:120915/1‑150 (MQ=255)

                                                                aGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCt                                                                               <  1:526589/151‑1 (MQ=255)

                                                                 ggCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTg                                                                              <  1:368021/151‑1 (MQ=255)

                                                                    cgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCg                                                                           >  1:530384/1‑151 (MQ=255)

                                                                       cTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATg                                                                        >  1:168543/1‑151 (MQ=255)

                                                                            ccTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCctct                                                                   <  2:457032/151‑1 (MQ=255)

                                                                                ttttGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTtcat                                                               >  2:164854/1‑151 (MQ=255)

                                                                                  ttGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTtcatc                                                              >  1:632062/1‑150 (MQ=255)

                                                                                  ttGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTtcatc                                                              <  2:575863/150‑1 (MQ=255)

                                                                                     tATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCAtcatt                                                           <  2:438883/150‑1 (MQ=255)

                                                                                            tCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGc                                                   >  2:182521/1‑151 (MQ=255)

                                                                                                aaCGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGt                                               <  2:498312/151‑1 (MQ=255)

                                                                                                     gTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCtt                                          <  2:411045/151‑1 (MQ=255)

                                                                                                       aaTGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTc                                         >  2:419840/1‑150 (MQ=255)

                                                                                                       aaTGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTc                                         >  2:510050/1‑150 (MQ=255)

                                                                                                                      ccGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTc                          <  1:646186/150‑1 (MQ=255)

                                                                                                                         cgTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGc                      >  2:667724/1‑151 (MQ=255)

                                                                                                                            ggtggCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTc                    <  2:169246/150‑1 (MQ=255)

                                                                                                                            ggtggCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTc                    <  2:416029/150‑1 (MQ=255)

                                                                                                                                    cGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGt           <  1:164821/151‑1 (MQ=255)

                                                                                                                                     ggAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTg          <  2:709471/151‑1 (MQ=255)

                                                                                                                                             aTCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGcc  >  1:412936/1‑151 (MQ=255)

GGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑GGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCC  >  NC_000913/3376192‑3376482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A