Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,376,341	+G	coding (436/498 nt)	sspB ←	ClpXP protease specificity enhancing factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,376,339	1	.	G	100.0%	140.7 / NA	37	P146P (CCT→CCC)	sspB	ClpXP protease specificity enhancing factor
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (18/19); total (18/19)

CGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑GGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTG  >  NC_000913/3376191‑3376478

cGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑gg                                                                                                                                           <  1:693131/151‑1 (MQ=255)

cGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑g                                                                                                                                            <  1:483874/150‑1 (MQ=255)

       gtggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAgt                                                                                                                                     >  2:612299/1‑151 (MQ=255)

       gtggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAg                                                                                                                                      >  1:563665/1‑150 (MQ=255)

         ggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAgtg                                                                                                                                    <  1:684746/150‑1 (MQ=255)

          gtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGtc                                                                                                                                  <  2:813302/151‑1 (MQ=255)

                atGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTcatcat                                                                                                                             <  1:891494/150‑1 (MQ=255)

                 tGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCg                                                                                                                           >  1:494499/1‑151 (MQ=255)

                  gATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCg                                                                                                                           <  1:420293/150‑1 (MQ=255)

                   aTTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTg                                                                                                                         >  1:386751/1‑151 (MQ=255)

                           aaTCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCt                                                                                                                 <  2:58588/151‑1 (MQ=255)

                                    aTTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGcc                                                                                                          <  2:529035/149‑1 (MQ=255)

                                       ttattaAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCa                                                                                                      <  1:202478/150‑1 (MQ=255)

                                             aGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCg                                                                                               <  2:381267/151‑1 (MQ=255)

                                               tCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGAc                                                                                             >  2:897258/1‑151 (MQ=255)

                                                cATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACa                                                                                            <  2:76641/151‑1 (MQ=255)

                                                      aaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACgg                                                                                      >  2:190577/1‑151 (MQ=255)

                                                      aaaGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACgg                                                                                      >  1:382285/1‑151 (MQ=255)

                                                         gACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGttt                                                                                   >  1:534218/1‑151 (MQ=255)

                                                          aCAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTc                                                                                  <  2:77152/151‑1 (MQ=255)

                                                              aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTg                                                                              <  1:881754/151‑1 (MQ=255)

                                                                   gccgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGc                                                                         <  1:556595/151‑1 (MQ=255)

                                                                           ggCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCct                                                                 <  1:471541/151‑1 (MQ=255)

                                                                              cTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCtt                                                              >  1:842685/1‑151 (MQ=255)

                                                                                   ttGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTtcatc                                                          >  2:38639/1‑150 (MQ=255)

                                                                                         tACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCAtcattcat                                                    <  1:531314/150‑1 (MQ=255)

                                                                                           cTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCatgat                                                 >  2:579389/1‑151 (MQ=255)

                                                                                               acaACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTg                                             >  2:711309/1‑151 (MQ=255)

                                                                                                     cgTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCt                                       <  1:645585/151‑1 (MQ=255)

                                                                                                           gCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATc                                  <  2:503450/150‑1 (MQ=255)

                                                                                                            ccGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGt                                <  1:268754/151‑1 (MQ=255)

                                                                                                             cGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTa                               <  1:379079/151‑1 (MQ=255)

                                                                                                                  gACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGc                            >  2:367194/1‑149 (MQ=255)

                                                                                                                      accGCGTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTc                      >  2:114242/1‑151 (MQ=255)

                                                                                                                          cgTGGTGGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGc                  >  1:227288/1‑151 (MQ=255)

                                                                                                                           gtggtgGCTGCGGAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCt                 >  2:169571/1‑151 (MQ=255)

                                                                                                                                      ggAGGTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTg      >  2:487486/1‑151 (MQ=255)

                                                                                                                                         ggTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCt   >  2:516603/1‑151 (MQ=255)

                                                                                                                                          gTTCATCGTCAGGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTg  <  2:649944/151‑1 (MQ=255)

CGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCA‑GGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTG  >  NC_000913/3376191‑3376478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A