Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,225,048	A→G	V105A (GTT→GCT)	minD ←	inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,225,048	0	A	G	100.0%	232.2 / NA	63	V105A (GTT→GCT)	minD	inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (33/30); total (33/30)

GTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACG  >  NC_000913/1224900‑1225194

gTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCt                                                                                                                                                  >  2:421462/1‑151 (MQ=255)

   agagTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTg                                                                                                                                               >  2:147690/1‑151 (MQ=255)

    gagTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTgg                                                                                                                                              >  2:851609/1‑151 (MQ=255)

     agTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGCTCATCAAGAGCTTTGGc                                                                                                                                             >  2:283718/1‑151 (MQ=255)

     agTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGc                                                                                                                                             <  1:790824/151‑1 (MQ=255)

             aCTGAGGAGACTTCCGGGTTGGTGGCAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCtt                                                                                                                                     >  2:681836/1‑151 (MQ=255)

                  ggagACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACgg                                                                                                                                <  1:679320/151‑1 (MQ=255)

                      aCTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGa                                                                                                                            <  1:593582/151‑1 (MQ=255)

                       cTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTg                                                                                                                             >  2:782720/1‑149 (MQ=255)

                         tCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAgg                                                                                                                          <  1:432355/150‑1 (MQ=255)

                         tCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAg                                                                                                                           <  1:833696/149‑1 (MQ=255)

                                    ggtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATcg                                                                                                              >  2:334660/1‑151 (MQ=255)

                                      taataaTGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATcgcg                                                                                                            >  2:816291/1‑151 (MQ=255)

                                       aataatGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATcgcg                                                                                                            <  2:252049/150‑1 (MQ=255)

                                       aataatGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAAGCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCGTCACGGGTGAGGGCATCTTTATCGCgt                                                                                                           <  2:855960/151‑1 (MQ=255)

                                              gCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGTGGAGTCACAAACTATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGTCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGAGTTTGc                                                                                                     <  2:306590/150‑1 (MQ=255)

                                              gCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCg                                                                                                    <  1:421507/151‑1 (MQ=255)

                                                ttCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGAt                                                                                                  >  1:566590/1‑151 (MQ=255)

                                                ttCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGAt                                                                                                  >  1:608168/1‑151 (MQ=255)

                                                  cGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGc                                                                                                >  2:429725/1‑151 (MQ=255)

                                                  cGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGc                                                                                                >  2:645421/1‑151 (MQ=255)

                                                   gTCTGCAAAATAGAGAGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTAAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGc                                                                                                >  2:808524/1‑150 (MQ=255)

                                                    tCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCg                                                                                              >  2:428351/1‑151 (MQ=255)

                                                      tGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGc                                                                                            >  1:539295/1‑151 (MQ=255)

                                                       gCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGc                                                                                            >  2:315277/1‑150 (MQ=255)

                                                          aaaTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGaa                                                                                        <  1:147713/151‑1 (MQ=255)

                                                            aTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGCCCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAata                                                                                      <  2:95942/151‑1 (MQ=255)

                                                            aTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAat                                                                                       >  1:95964/1‑150 (MQ=255)

                                                            aTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAat                                                                                       >  1:210761/1‑150 (MQ=255)

                                                              agagTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAAtata                                                                                    <  2:778078/151‑1 (MQ=255)

                                                                 gTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATag                                                                                   >  1:184877/1‑149 (MQ=255)

                                                                   gCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATag                                                                                   >  1:872138/1‑147 (MQ=255)

                                                                     cATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAttt                                                                             <  1:98951/151‑1 (MQ=255)

                                                                      aTTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAtttt                                                                            >  1:297012/1‑151 (MQ=255)

                                                                         aaCGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCa                                                                          <  2:31163/150‑1 (MQ=255)

                                                                            gCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTAc                                                                      <  2:3672/151‑1 (MQ=255)

                                                                                cGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCATTACGCtt                                                                  <  2:618457/151‑1 (MQ=255)

                                                                                 ggTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTa                                                                 <  1:48389/151‑1 (MQ=255)

                                                                                  gTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTAt                                                                <  2:577095/151‑1 (MQ=255)

                                                                                    ttCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATc                                                               <  1:727158/150‑1 (MQ=255)

                                                                                    ttCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATc                                                               <  2:427423/150‑1 (MQ=255)

                                                                                      cAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCtt                                                             <  1:217549/150‑1 (MQ=255)

                                                                                       aaTCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCttt                                                            >  2:825442/1‑150 (MQ=255)

                                                                                          cccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTttaat                                                         <  2:901/150‑1 (MQ=255)

                                                                                           ccTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGAACGCTTATCTttaatta                                                       <  1:350807/151‑1 (MQ=255)

                                                                                             tGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAAc                                                     >  1:743872/1‑151 (MQ=255)

                                                                                                          caAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCg                                        <  1:364391/151‑1 (MQ=255)

                                                                                                            aaCGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGtt                                      >  2:727236/1‑151 (MQ=255)

                                                                                                            aaCGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGtt                                      <  1:367232/151‑1 (MQ=255)

                                                                                                               gATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCa                                   <  2:840962/151‑1 (MQ=255)

                                                                                                                    aTTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGcc                              >  1:441036/1‑151 (MQ=255)

                                                                                                                             ccATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGAc                     <  2:421907/151‑1 (MQ=255)

                                                                                                                                 cGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTg                 >  1:789855/1‑151 (MQ=255)

                                                                                                                                  gCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTg                 >  1:382976/1‑150 (MQ=255)

                                                                                                                                  gCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTg                 >  2:731547/1‑150 (MQ=255)

                                                                                                                                   cTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGAc               >  1:236180/1‑151 (MQ=255)

                                                                                                                                    tttCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACg              >  1:70789/1‑151 (MQ=255)

                                                                                                                                      tCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGa             >  1:888602/1‑150 (MQ=255)

                                                                                                                                         gATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATc         <  2:147970/151‑1 (MQ=255)

                                                                                                                                               caAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTaaa    >  2:464120/1‑150 (MQ=255)

                                                                                                                                               caAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAAc   <  1:469623/151‑1 (MQ=255)

                                                                                                                                                aaGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAAc   <  2:61996/150‑1 (MQ=255)

                                                                                                                                                aaGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACg  >  2:267158/1‑151 (MQ=255)

GTCAGAGTCGCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACG  >  NC_000913/1224900‑1225194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A