Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,225,048 A→G V105A (GTT→GCT)  minD ← inhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,225,0480AG100.0% 186.1 / NA 49V105A (GTT→GCT) minDinhibitor of FtsZ ring polymerization; chromosome‑membrane tethering protein; membrane ATPase of the MinCDEE system
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

GCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTA  >  NC_000913/1224909‑1225190
                                                                                                                                           |                                                                                                                                              
gcgTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGAcc                                                                                                                                     >  1:219653/1‑151 (MQ=255)
  gTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGAcccc                                                                                                                                   <  1:627751/151‑1 (MQ=255)
    aCTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCtt                                                                                                                                 >  2:564513/1‑151 (MQ=255)
      tGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTc                                                                                                                                >  2:68051/1‑150 (MQ=255)
           agaCTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGt                                                                                                                          <  1:252385/151‑1 (MQ=255)
                           ggtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATcg                                                                                                          >  2:614550/1‑151 (MQ=255)
                                  atGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGttt                                                                                                   <  2:159850/151‑1 (MQ=255)
                                   tGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTg                                                                                                  >  2:392365/1‑151 (MQ=255)
                                    ggCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTg                                                                                                  <  1:157198/150‑1 (MQ=255)
                                        tCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGAt                                                                                              <  1:570206/150‑1 (MQ=255)
                                         cGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATg                                                                                             <  2:55445/150‑1 (MQ=255)
                                          gTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGcc                                                                                           >  1:60959/1‑151 (MQ=255)
                                          gTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGcc                                                                                           >  2:566766/1‑151 (MQ=255)
                                              gCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGc                                                                                        <  1:572790/150‑1 (MQ=255)
                                                aaaaTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGa                                                                                     <  1:275/151‑1 (MQ=255)
                                                aaaaTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTCATCGCGTGTTTGCGATGCCGGc                                                                                        >  1:539113/1‑148 (MQ=255)
                                                 aaaTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGaa                                                                                    <  2:89542/151‑1 (MQ=255)
                                                 aaaTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGaa                                                                                    <  2:699343/151‑1 (MQ=255)
                                                    tAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAatat                                                                                 >  2:266191/1‑151 (MQ=255)
                                                     agagTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAatat                                                                                 <  2:694797/150‑1 (MQ=255)
                                                     agagTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAAtata                                                                                <  2:286634/151‑1 (MQ=255)
                                                      gagTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAatat                                                                                 >  2:203686/1‑149 (MQ=255)
                                                       agTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATaga                                                                              <  1:350814/151‑1 (MQ=255)
                                                        gTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATag                                                                               >  1:29143/1‑149 (MQ=255)
                                                                aaCGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCa                                                                      <  1:492398/150‑1 (MQ=255)
                                                                  cGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTa                                                                   <  2:140320/151‑1 (MQ=255)
                                                                     aCCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGc                                                                >  1:178827/1‑151 (MQ=255)
                                                                        ggTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCtt                                                              >  2:751089/1‑150 (MQ=255)
                                                                         gTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTAt                                                            <  2:432759/151‑1 (MQ=255)
                                                                              aaTCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCttt                                                        >  2:528338/1‑150 (MQ=255)
                                                                              aaTCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCttt                                                        <  1:679921/150‑1 (MQ=255)
                                                                               aTCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTttaa                                                      <  1:556459/151‑1 (MQ=255)
                                                                                     gCCGGGTAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTttaattaa                                                  <  2:185866/149‑1 (MQ=255)
                                                                                     gCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAAc                                                 <  2:680987/150‑1 (MQ=255)
                                                                                     gCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAAc                                                 <  1:385246/150‑1 (MQ=255)
                                                                                     gCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACg                                                <  1:203654/151‑1 (MQ=255)
                                                                                                          aaTTCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGc                           >  2:323217/1‑151 (MQ=255)
                                                                                                             tCAAAATCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCt                        >  1:217000/1‑151 (MQ=255)
                                                                                                               aaaaTCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTg                       >  2:717685/1‑150 (MQ=255)
                                                                                                                aaaTCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGa                      >  1:666239/1‑150 (MQ=255)
                                                                                                                  aTCCATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATg                   <  2:767308/151‑1 (MQ=255)
                                                                                                                    ccATCGCTTTCAGAGAATCAAGAGAGTGAGCGACCGCTTCAAGGGTGAGGACATCTTTATCGCGTGGTTGGGATGCCGGCAGAATATAGAGATTGTCAGTACGCTTATCGTTATTTATCGACTGAGTTAGCGTTGCATCGCCCTGCAt                    >  1:374587/1‑148 (MQ=255)
                                                                                                                     cATCGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACg                >  2:468313/1‑151 (MQ=255)
                                                                                                                        cGCTTTCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGtt              <  1:719019/150‑1 (MQ=255)
                                                                                                                             tCAGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGa         <  2:147038/150‑1 (MQ=255)
                                                                                                                               aGATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAAt      >  1:696488/1‑151 (MQ=255)
                                                                                                                                gATCATCAAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATc     >  1:386872/1‑151 (MQ=255)
                                                                                                                                  tcatcaAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCGTCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGt   >  2:262754/1‑151 (MQ=255)
                                                                                                                                   catcaAGAGCTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTa  <  1:300384/151‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                              
GCGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTA  >  NC_000913/1224909‑1225190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A