Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,819,577 A→G V812A (GTT→GCT)  alaS ← alanyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,819,5770AG100.0% 187.2 / NA 53V812A (GTT→GCT) alaSalanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

GGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCC  >  NC_000913/2819430‑2819720
                                                                                                                                                   |                                                                                                                                                
ggTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcct                                                                                                                                               <  2:635829/151‑1 (MQ=255)
 gTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcctt                                                                                                                                              <  1:221095/151‑1 (MQ=255)
          cagcaTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGAc                                                                                                                                     >  1:593634/1‑151 (MQ=255)
           agcaTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACt                                                                                                                                    >  1:841402/1‑151 (MQ=255)
           agcaTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACt                                                                                                                                    >  2:327252/1‑151 (MQ=255)
               tCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCg                                                                                                                                >  1:473514/1‑151 (MQ=255)
               tCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCg                                                                                                                                >  2:761379/1‑151 (MQ=255)
                 cctACCACCGCATTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTCCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATATGTTACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGtt                                                                                                                              >  2:432648/3‑151 (MQ=255)
                 cGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGtt                                                                                                                              <  1:699396/151‑1 (MQ=255)
                    accaccGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGcc                                                                                                                           <  1:330834/151‑1 (MQ=255)
                          ggCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACg                                                                                                                     <  2:210351/151‑1 (MQ=255)
                                    aTGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTc                                                                                                            <  2:723239/150‑1 (MQ=255)
                                         aGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGAcccc                                                                                                      <  1:758197/151‑1 (MQ=255)
                                              gTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTg                                                                                                              <  2:730504/138‑1 (MQ=255)
                                              gTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTg                                                                                                              >  1:730553/1‑138 (MQ=255)
                                               tCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTg                                                                                                 >  2:669270/1‑150 (MQ=255)
                                                     accCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtttttt                                                                                          <  1:668984/151‑1 (MQ=255)
                                                           gccgccCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATc                                                                                     <  1:177264/150‑1 (MQ=255)
                                                           gccgccCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCg                                                                                    <  1:558073/151‑1 (MQ=255)
                                                                ccACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAc                                                                               >  1:594191/1‑151 (MQ=255)
                                                                ccACCTGCTGACCGACCATCCCAATCAATTCCCCTGCTTTCACACGATCTGTGACTTCCTTATATACGCCTGCAATCAGAGAAGCCTTATCTTCGTCTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCg                                                                                    <  2:894322/146‑1 (MQ=255)
                                                                   ccTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CAt                                                                           >  2:282443/1‑151 (MQ=255)
                                                                     tgctgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTTTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATgg                                                                         >  1:263604/1‑151 (MQ=255)
                                                                      gctgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATg                                                                          <  1:45443/149‑1 (MQ=255)
                                                                      gctgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGt                                                                        <  2:220084/151‑1 (MQ=255)
                                                                         gAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTa                                                                       <  2:738941/149‑1 (MQ=255)
                                                                         gAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACg                                                                     >  2:329071/1‑151 (MQ=255)
                                                                          aGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGc                                                                    >  1:719498/1‑151 (MQ=255)
                                                                            cGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCa                                                                   >  1:220167/1‑150 (MQ=255)
                                                                             gACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAAc                                                                 >  1:88792/1‑151 (MQ=255)
                                                                                  tACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACAtttt                                                            >  2:260508/1‑151 (MQ=255)
                                                                                   aCCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACAtttt                                                            <  2:393730/150‑1 (MQ=255)
                                                                                   aCCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTc                                                           <  2:720286/151‑1 (MQ=255)
                                                                                   aCCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTc                                                           <  2:49778/151‑1 (MQ=255)
                                                                                    ccAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCCACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTc                                                           <  2:431000/150‑1 (MQ=255)
                                                                                     cAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCgg                                                         <  2:494197/151‑1 (MQ=255)
                                                                                      aaTCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGc                                                        <  1:351746/151‑1 (MQ=255)
                                                                                             tCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAacac                                                 >  2:232812/1‑151 (MQ=255)
                                                                                              ccccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACAcc                                                <  1:706940/151‑1 (MQ=255)
                                                                                                ccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGc                                              >  1:429776/1‑151 (MQ=255)
                                                                                                ccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGc                                              >  1:721993/1‑151 (MQ=255)
                                                                                                   gCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTa                                            >  2:645622/1‑150 (MQ=255)
                                                                                                      ttCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCt                                        >  1:168276/1‑151 (MQ=255)
                                                                                                      ttCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCt                                        >  1:152988/1‑151 (MQ=255)
                                                                                                           acGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTa                                   <  2:909867/151‑1 (MQ=255)
                                                                                                                  gtgACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCa                               <  1:874448/148‑1 (MQ=255)
                                                                                                                   tgacgggcatAGATACGCATGCAATCAGAGAAGCCTTATCTTCGACGGCCGTTGCCAGCACGATAATTGTCGGCTCCCGTTGATTTTTTAACTCGTCACCTTATGG‑ACGCACCATTGTCGGATCAACACCGCCAAGCTCGCTacc                                 >  2:778292/10‑143 (MQ=255)
                                                                                                                   tgACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAAc                             >  2:114843/1‑149 (MQ=255)
                                                                                                                      cGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCtt                        <  2:577647/151‑1 (MQ=255)
                                                                                                                       gTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCtt                        >  1:94913/1‑150 (MQ=255)
                                                                                                                        tCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTa                       >  1:96802/1‑150 (MQ=255)
                                                                                                                         ccTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAac                     >  2:494751/1‑151 (MQ=255)
                                                                                                                               aTACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCAtt               <  2:98272/151‑1 (MQ=255)
                                                                                                                                 aCGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTaa             <  2:499583/151‑1 (MQ=255)
                                                                                                                                             agagAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGcc  >  2:624335/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAC‑CATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCC  >  NC_000913/2819430‑2819720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A