Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,819,577	A→G	V812A (GTT→GCT)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,819,577	0	A	G	100.0%	144.2 / NA	40	V812A (GTT→GCT)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/25); total (15/25)

AGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTT  >  NC_000913/2819429‑2819722

aGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCTCCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcc                                                                                                                                                 <  2:444295/151‑1 (MQ=255)

aGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcc                                                                                                                                                 <  1:14175/151‑1 (MQ=255)

aGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAgcc                                                                                                                                                 >  2:254106/1‑151 (MQ=255)

               aTCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTAcc                                                                                                                                  >  1:364576/1‑151 (MQ=255)

                    tACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGc                                                                                                                             >  1:83939/1‑151 (MQ=255)

                          cGGCTTGCGCCAGGTCAGGACGTCCACCCCCCTTGCCGCCCACCTGCTGAGCGACCATAACAATCAGTTCCCCTGCTTTCCCACGATCTGTGACGTCCTTAGATACGCCTGCAAACAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCAc                                                                                                                       <  1:405114/151‑1 (MQ=255)

                            gCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGa                                                                                                                     <  2:703636/151‑1 (MQ=255)

                                gcgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATaa                                                                                                                  <  2:598232/150‑1 (MQ=255)

                                gcgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAAt                                                                                                                 <  1:254073/151‑1 (MQ=255)

                                gcgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAAt                                                                                                                 >  2:201551/1‑151 (MQ=255)

                                 cgcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAAtt                                                                                                                <  2:720686/151‑1 (MQ=255)

                                  gcCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTg                                                                                                               >  2:754266/1‑151 (MQ=255)

                                    catctcattaCGACCACCACCCCTTCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACCAGCCCTGACGTCCTCAGATACGCCTACAATCAGAGAAGCCGTTCCTTCGACTACCGTTGCCTGCACGATAATTGtt                                                                                                             <  1:542856/142‑2 (MQ=255)

                                           ggACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCa                                                                                                      <  2:258607/151‑1 (MQ=255)

                                                 ccaccaCCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAt                                                                                                <  2:210495/151‑1 (MQ=255)

                                                  caccacCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtt                                                                                               <  1:386384/151‑1 (MQ=255)

                                                  caccacCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtt                                                                                               >  1:40946/1‑151 (MQ=255)

                                                   accaccCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAttt                                                                                              <  1:549229/151‑1 (MQ=255)

                                                    ccaccCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAtttt                                                                                             <  2:47127/151‑1 (MQ=255)

                                                     caccCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGAttttt                                                                                            >  1:217884/1‑151 (MQ=255)

                                                       cccTTGCCGCCCACCTGCTGAGCGACCATACCAAGCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTa                                                                                          <  2:483892/151‑1 (MQ=255)

                                                                cccACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCa                                                                                  >  1:418377/1‑150 (MQ=255)

                                                                 ccACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAAc                                                                                <  1:4646/151‑1 (MQ=255)

                                                                         tgAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTa                                                                         >  1:602574/1‑150 (MQ=255)

                                                                                ccATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACAt                                                                 <  2:289421/151‑1 (MQ=255)

                                                                                ccATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACAt                                                                 >  2:359907/1‑151 (MQ=255)

                                                                                 cATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACAtt                                                                >  1:100462/1‑151 (MQ=255)

                                                                                                 ccTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGc                                                <  2:187442/151‑1 (MQ=255)

                                                                                                    gCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTa                                              <  2:552727/150‑1 (MQ=255)

                                                                                                      tttCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGc                                           >  2:208787/1‑151 (MQ=255)

                                                                                                        tCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTc                                         >  2:734892/1‑151 (MQ=255)

                                                                                                         cacaCGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTc                                         <  2:273215/150‑1 (MQ=255)

                                                                                                           cacGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCt                                      <  1:602941/151‑1 (MQ=255)

                                                                                                           cacGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCt                                      <  2:29851/151‑1 (MQ=255)

                                                                                                                 cTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCa                                 >  2:435169/1‑150 (MQ=255)

                                                                                                                   gtgACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACa                              <  1:490909/151‑1 (MQ=255)

                                                                                                                     gACGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGc                            <  2:137957/151‑1 (MQ=255)

                                                                                                                      aCGTCCTTAGATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATGTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGc                            <  1:342471/150‑1 (MQ=255)

                                                                                                                               gATACGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCAt                  <  2:689810/151‑1 (MQ=255)

                                                                                                                                  aCGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTa                <  2:269464/150‑1 (MQ=255)

                                                                                                                                  aCGCCTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTa                >  1:482629/1‑150 (MQ=255)

                                                                                                                                     ccTGCAATCAGAGAAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACAt            >  1:189601/1‑151 (MQ=255)

                                                                                                                                               gagaAGCCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCtt  <  1:548597/151‑1 (MQ=255)

AGGTAAGGCCGCAGCATCCGTACCACCGGCTTGCGCCATGTCAGGACGTCCACCACCCTTGCCGCCCACCTGCTGAGCGACCATACCAATCAGTTCCCCTGCTTTCACACGATCTGTGACGTCCTTAGATACGCCTGCAATCAGAGAAACCTTACCTTCGACTACCGTTGCCAGCACGATAATTGTCGACCCCAGCTGATTTTTTAAATCGTCAACCATGGTACGCAACATTTTCGGCTCAACACCGCTAAGCTCGCTAACCAACAGCTTAACACCATTAACATCAATTGCCTT  >  NC_000913/2819429‑2819722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A