Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,049,805 (T)5→6 coding (863/1095 nt) gcvT ← aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,049,8001.T97.8% 174.1 / ‑3.2 46coding (868/1095 nt)gcvTaminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (20/25);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

CGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTT  >  NC_000913/3049654‑3049943
                                                                                                                                                   |                                                                                                                                                
cGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑tttt                                                                                                                                              <  2:93548/151‑1 (MQ=255)
cGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑tttt                                                                                                                                              <  1:383226/151‑1 (MQ=255)
 gCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttt                                                                                                                                             <  2:287675/151‑1 (MQ=255)
    gTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttttcg                                                                                                                                          <  2:179553/151‑4 (MQ=255)
      ttCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttttcgg                                                                                                                                         <  1:197330/150‑5 (MQ=255)
        cGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGtc                                                                                                                                       <  1:650941/150‑1 (MQ=255)
               aCCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAg                                                                                                                               <  1:606000/151‑1 (MQ=255)
                 cTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGac                                                                                                                             >  1:195514/1‑151 (MQ=255)
                   tCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGac                                                                                                                             <  1:393383/149‑1 (MQ=255)
                              cgcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGttttt                                                                                                                 >  2:857965/1‑150 (MQ=255)
                               gcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCt                                                                                                               <  2:757815/151‑1 (MQ=255)
                                 cAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGt                                                                                                             >  1:235053/1‑151 (MQ=255)
                                 cAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGt                                                                                                             >  1:388237/1‑151 (MQ=255)
                                    cgcAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTAcc                                                                                                          >  2:601903/1‑151 (MQ=255)
                                      cAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCAt                                                                                                        >  2:887966/1‑151 (MQ=255)
                                       aaTGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATg                                                                                                       >  1:288025/1‑151 (MQ=255)
                                          gCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCt                                                                                                     <  1:794037/150‑1 (MQ=255)
                                             gTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCAc                                                                                                  <  1:345001/150‑1 (MQ=255)
                                             gTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACg                                                                                                 <  1:619397/151‑1 (MQ=255)
                                                          ggggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAgg                                                                                    <  1:905142/151‑1 (MQ=255)
                                                           gggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAggg                                                                                   <  1:322993/151‑1 (MQ=255)
                                                           gggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTTCCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAggg                                                                                   <  1:599819/151‑1 (MQ=255)
                                                            ggAGAAAGTGCCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCGCCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGc                                                                                  <  2:133680/151‑1 (MQ=255)
                                                             gagaAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCt                                                                                 >  1:890025/1‑151 (MQ=255)
                                                              agaAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCtt                                                                                <  1:764251/151‑1 (MQ=255)
                                                                        gCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGAt                                                                      >  1:671443/1‑151 (MQ=255)
                                                                           ggTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATaaa                                                                   <  1:179397/151‑1 (MQ=255)
                                                                              gATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGt                                                                 <  2:657216/150‑1 (MQ=255)
                                                                               aTAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCa                                                               <  2:697994/151‑1 (MQ=255)
                                                                                          aTGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCgg                                                    >  2:53891/1‑151 (MQ=255)
                                                                                                 ttGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTccc                                               >  2:58482/1‑149 (MQ=255)
                                                                                                   gCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGc                                           >  1:667320/1‑151 (MQ=255)
                                                                                                       tGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCAACACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATg                                       >  2:664192/1‑151 (MQ=255)
                                                                                                          gcATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTc                                    >  2:196057/1‑151 (MQ=255)
                                                                                                            aTCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTcc                                   >  2:124770/1‑150 (MQ=255)
                                                                                                                gTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGccc                              >  1:234982/1‑151 (MQ=255)
                                                                                                                   aaGCGTACCGGCATTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGGCCATg                           <  2:788370/151‑1 (MQ=255)
                                                                                                                   aaGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATg                           >  1:352737/1‑151 (MQ=255)
                                                                                                                        tACCGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggc                      >  1:187642/1‑151 (MQ=255)
                                                                                                                          ccGGCAGTTCATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCGGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggc                      <  1:8128/149‑1 (MQ=255)
                                                                                                                                   cATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGga            <  1:20112/150‑1 (MQ=255)
                                                                                                                                   cATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGga            <  1:61163/150‑1 (MQ=255)
                                                                                                                                   cATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGga            <  2:67010/150‑1 (MQ=255)
                                                                                                                                   cATTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCACGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgag           <  2:356810/151‑1 (MQ=255)
                                                                                                                                    aTTACGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgaga          <  1:803695/151‑1 (MQ=255)
                                                                                                                                       aCGCAGCACGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgagaga        <  1:352091/150‑1 (MQ=255)
                                                                                                                                          cagcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGt    >  2:55237/1‑151 (MQ=255)
                                                                                                                                          cagcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGt    <  1:132352/151‑1 (MQ=255)
                                                                                                                                           agcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATggct   >  2:885021/1‑149 (MQ=255)
                                                                                                                                           agcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGtt   <  1:767371/151‑1 (MQ=255)
                                                                                                                                            gcaCGCCTTTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGttt  <  2:592179/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTT‑CGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTT  >  NC_000913/3049654‑3049943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A