Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,049,805	(T)_5→6	coding (863/1095 nt)	gcvT ←	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,049,800	1	.	T	100.0%	119.2 / NA	33	G290S (GGC→AGC)	gcvT	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (12/21); total (12/21)

AATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGG  >  NC_000913/3049651‑3049932

aaTCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑t                                                                                                                                     <  2:572905/151‑1 (MQ=255)

          tCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttttcggt                                                                                                                            <  2:194982/150‑6 (MQ=255)

           cGCCAATGCCTTCCAGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGtc                                                                                                                            <  1:269162/150‑1 (MQ=255)

           cGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGtca                                                                                                                           <  2:292561/151‑1 (MQ=255)

            gCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGtcat                                                                                                                          <  1:255058/151‑1 (MQ=255)

                 tACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGGGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCAcc                                                                                                                      >  1:5829/1‑150 (MQ=255)

                   ccTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGa                                                                                                                   <  1:634060/151‑1 (MQ=255)

                        cGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGaccaac                                                                                                              >  1:689790/1‑151 (MQ=255)

                                 cgcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTc                                                                                                     <  1:61056/151‑1 (MQ=255)

                                  gcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTc                                                                                                     >  1:468341/1‑150 (MQ=255)

                                     aGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTa                                                                                                 <  2:769742/151‑1 (MQ=255)

                                         cAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCAt                                                                                             <  2:217036/151‑1 (MQ=255)

                                                  aaCCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGc                                                                                     <  2:179785/150‑1 (MQ=255)

                                                    cccAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGc                                                                                  <  1:280295/151‑1 (MQ=255)

                                                            cGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAg                                                                          <  1:567231/151‑1 (MQ=255)

                                                             ggggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGCGTGCCCGGCGCATGGGCAAAGCGTACCGGGAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAgg                                                                         >  1:125186/1‑151 (MQ=255)

                                                                   aaaGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCa                                                                   <  2:429808/151‑1 (MQ=255)

                                                                    aaGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCAc                                                                  >  2:26072/1‑151 (MQ=255)

                                                                         ccGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCg                                                             <  2:76059/151‑1 (MQ=255)

                                                                               gTGATAATGCCTTCATTCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTGCCAGGGCTTCCCGACCGATat                                                         <  1:469781/149‑2 (MQ=255)

                                                                                tGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGt                                                      >  2:233696/1‑151 (MQ=255)

                                                                                  aTAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTc                                                     >  2:26885/1‑150 (MQ=255)

                                                                                                      gCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGc                                <  1:4741/151‑1 (MQ=255)

                                                                                                        ccTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCg                               >  1:558899/1‑150 (MQ=255)

                                                                                                           gcgcATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGAt                             <  1:378896/149‑1 (MQ=255)

                                                                                                                 cGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGc                     >  1:322300/1‑151 (MQ=255)

                                                                                                                   gTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGccc                   <  1:53503/151‑1 (MQ=255)

                                                                                                                      aaGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATg                <  1:744733/151‑1 (MQ=255)

                                                                                                                             ccGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggcgg         <  1:261800/151‑1 (MQ=255)

                                                                                                                             ccGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggcgg         >  2:303841/1‑151 (MQ=255)

                                                                                                                               ggCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCt       <  1:653303/151‑1 (MQ=255)

                                                                                                                               ggCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCt       <  1:191064/151‑1 (MQ=255)

                                                                                                                                 cAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCCGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTa      >  2:650945/1‑150 (MQ=255)

                                                                                                                                     tCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAgg  >  2:457732/1‑150 (MQ=255)

                                                                                                                                     tCACTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATTCTCACGCTGCACTTCCAGAGCTTCGCGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAgg  <  1:206160/150‑1 (MQ=255)

AATCGCCGTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGG  >  NC_000913/3049651‑3049932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A