Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,428	T→C	E438E (GAA→GAG)	uxaC ←	uronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,428	0	T	C	100.0%	190.8 / NA	53	E438E (GAA→GAG)	uxaC	uronate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/19); total (34/19)

ACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTTCCA  >  NC_000913/3243280‑3243573

aCGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTc                                                                                                                                                 >  1:643805/1‑151 (MQ=255)

             cagggttCTTGATGTATTGCATATCAACCCCAGACCTTGTTTCAGTTCAAGGGCGAAGTAGTCCCGCGCATTGTTAACGAAAATTTTTTTCCCCACCCCGCCGAGCGGGTTGTGGTCGGCCGGTGCTTTGCCCGCCTCCACCCAGCGAcc                                                                                                                                     <  2:439034/144‑1 (MQ=255)

                      tGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCAAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTgg                                                                                                                           >  2:20668/1‑151 (MQ=255)

                          gTATTGCATATCACCCCCAGCCCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGcac                                                                                                                        <  1:729104/150‑1 (MQ=255)

                                 atatCACCCCCAGTCCTTAGTACAGTTCAATGCCGAAGTAGTCCCGCACATTTTTAAAGCAAATATTTCTCCCCTTCCCGCCCCACAGGTTGATTTCCCCCGGTTCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcg                                                                                                                <  1:20690/151‑1 (MQ=255)

                                    tCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcggcg                                                                                                             >  1:215685/1‑151 (MQ=255)

                                     cAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGcggcgg                                                                                                            >  2:786914/1‑151 (MQ=255)

                                      aaCCCCAGACCAGAGTTCAGTTCAATGGAGAAGTAGTCACGCGCATTGTAAAAGCAAAGATTTTTCACAATCCCGCCCAGCAGGTTAATGCCCGCCGGTGCTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcggc                                                                                                              <  1:353573/148‑1 (MQ=255)

                                       aCCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGa                                                                                                           >  1:799926/1‑150 (MQ=255)

                                         cccAGCCCTTAGTTCAGTTCAAGGGCGAAGTAGTCACGCGCATTTTTAAAGCAAATATTTTTCCCCACCCCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGt                                                                                                        <  2:404889/151‑1 (MQ=255)

                                          ccAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGt                                                                                                        >  1:818850/1‑150 (MQ=255)

                                                     ttcagttcaAGGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTCCCCCACCCCCCCCAGCAGGTTGATGCCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACg                                                                                              <  2:240306/149‑1 (MQ=255)

                                                            caATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATg                                                                                      >  2:348705/1‑150 (MQ=255)

                                                             aagggCGAAGTATTCCCGCGCATTGTTAAAGAAAATTTTTTCCCCCATCCCGCCCAGCGGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGAc                                                                                    <  2:570062/148‑1 (MQ=255)

                                                             aagggCGAAGTAGTCACGCGCATTTTAAAAGAAAATTTTTTCCACCATCCCCCCAAGCAGTTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGAc                                                                                    <  2:186545/148‑1 (MQ=255)

                                                             aaTGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGAc                                                                                    >  1:527293/1‑151 (MQ=255)

                                                              aTGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGAc                                                                                    >  1:400854/1‑150 (MQ=255)

                                                                ggCGAAGTAGTCACGCGCTTTGTTAAAGAAAATATTTTTCCCCTTCTCCCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAgg                                                                                 <  2:753605/151‑1 (MQ=255)

                                                                ggCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAgg                                                                                 >  2:726731/1‑151 (MQ=255)

                                                                     ccgatctaCGCGCATTGTTAAAGCAAATATTTTTCCCCATCCCGCCCAGCAGGTTGATGCCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAg                                                                              <  2:592866/142‑1 (MQ=255)

                                                                      gtagtCACGCGCATTGTTAAAGCAAATATTTTCCACCATCTCGCCCAGCAGGTTAATTTCCGCCGGTGTTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGaa                                                                               <  1:878082/147‑1 (MQ=255)

                                                                            aCGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAg                                                                              >  1:592915/1‑142 (MQ=255)

                                                                              gcgcATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtc                                                                   >  2:543070/1‑151 (MQ=255)

                                                                               cgcATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtc                                                                   >  2:333329/1‑150 (MQ=255)

                                                                                gcATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtc                                                                   >  2:740649/1‑149 (MQ=255)

                                                                                   ttGTTAAAGCAAATATTTTTCCCCAACTCGCCCAGCAGGTTGATGTCCGCCGGTGCTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtcag                                                                 <  1:662564/148‑1 (MQ=255)

                                                                                      ttAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGc                                                            >  2:599771/1‑150 (MQ=255)

                                                                                       tAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCAt                                                          >  1:329969/1‑151 (MQ=255)

                                                                                       tAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCAt                                                          >  2:417613/1‑151 (MQ=255)

                                                                                        aaaGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCAt                                                          >  1:778000/1‑150 (MQ=255)

                                                                                             aaaTATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAc                                                    >  1:391088/1‑151 (MQ=255)

                                                                                               atatTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAc                                                    >  2:56302/1‑149 (MQ=255)

                                                                                                tatTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACCGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGa                                                     >  2:562628/1‑147 (MQ=255)

                                                                                                 atTTTTCACCATCTCGCCCAGCAGGGTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGAGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACa                                                   >  1:178301/1‑148 (MQ=255)

                                                                                                   ttttCCCCATCCCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGc                                               <  1:870856/150‑1 (MQ=255)

                                                                                                    tttCCCCATCCCCCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCg                                              <  1:516321/150‑1 (MQ=255)

                                                                                                    tttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCgg                                             >  2:486992/1‑151 (MQ=255)

                                                                                                     ttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGc                                            >  2:861131/1‑151 (MQ=255)

                                                                                                     ttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGc                                            >  2:868165/1‑151 (MQ=255)

                                                                                                     ttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGAGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGc                                            >  1:139899/1‑151 (MQ=255)

                                                                                                         ccaccccccccAGCAGGTTGATGTCGGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCt                                           <  2:853780/140‑1 (MQ=255)

                                                                                                         ccATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTc                                          >  2:803913/1‑149 (MQ=255)

                                                                                                             ctcGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagc                                       >  2:175141/1‑148 (MQ=255)

                                                                                                             cccccccGGCGGGTTGATGCCCCCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagca                                      <  2:2868/145‑1 (MQ=255)

                                                                                                                  ccAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCg                                 >  2:183629/1‑149 (MQ=255)

                                                                                                                   cagcagGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGc                              >  1:876278/1‑151 (MQ=255)

                                                                                                                       agGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTgcg                          >  1:535006/1‑151 (MQ=255)

                                                                                                                        ggTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTgcgc                         >  1:402973/1‑151 (MQ=255)

                                                                                                                             aTGCCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGt                     <  1:778519/150‑1 (MQ=255)

                                                                                                                              tGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGAACGAGCTGCGCCAGtt                    >  2:553779/1‑150 (MQ=255)

                                                                                                                                tCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTggg                 >  1:663508/1‑151 (MQ=255)

                                                                                                                                               cccGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGtt     <  1:15534/148‑1 (MQ=255)

                                                                                                                                               cccGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTTCCa  <  2:564349/151‑1 (MQ=255)

ACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTTCCA  >  NC_000913/3243280‑3243573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A