Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,243,428	T→C	E438E (GAA→GAG)	uxaC ←	uronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,243,428	0	T	C	100.0%	163.4 / NA	46	E438E (GAA→GAG)	uxaC	uronate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/19); total (27/19)

ACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTT  >  NC_000913/3243280‑3243570

aCGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTc                                                                                                                                              >  1:155705/1‑151 (MQ=255)

                 gATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGatc                                                                                                                              >  1:421537/1‑150 (MQ=255)

                       gATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGc                                                                                                                       >  2:312294/1‑151 (MQ=255)

                        atttATTGCATATCACCCCCAGACATTAGTTCAGTTAAGCGGAGAAGTAGTCACGCGCATGGTTAAAGAAAATATTTTCCACCACCTCCCCGAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGc                                                                                                                       <  1:756416/147‑1 (MQ=255)

                               gCATATCACCCCCAGACCTTAGTTCAGTTCAAGGGCGAAGTAGTCACGCGCATCGTTAAAGCAAATATTTTTCCCCACCCCGCCCAGCGGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAAt                                                                                                                <  1:664673/150‑1 (MQ=255)

                                 atatCAACCCCAGCCCTTAGTTCAGTTCAATGGCGAAGTAGTCCCGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgc                                                                                                              <  2:200776/150‑1 (MQ=255)

                                  tatCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcgg                                                                                                            >  2:516900/1‑151 (MQ=255)

                                     cAACCCCAGACCTTATTGCAGTTAAATGGAGAAGTAGTCCCGCGCATTTTTAAAGCAAATTTTTTCCCCCACCTCGCCACGCAGGTTGATGTCCCCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcggc                                                                                                           <  1:505575/149‑1 (MQ=255)

                                     cAACCCCAGACCTTAGTTCAGTTCAAGGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATgcggcg                                                                                                          <  2:59055/150‑1 (MQ=255)

                                        ccccATACTTTAGTTCAGTTCAATGGCCAATGGGCCGCGCGCATTTTTAAAGCAAATATTTGTCCCCATCCCGCCCGGGAGGTTGATGCCCGCCGGGCGTGGGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGaa                                                                                                       <  1:555256/150‑1 (MQ=255)

                                             gACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCCCGCCCAGCAGGTTGATGTCCGCGGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTAtt                                                                                                  <  2:420409/150‑1 (MQ=255)

                                               ccTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGt                                                                                               >  2:738392/1‑151 (MQ=255)

                                                cTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGt                                                                                               >  2:613679/1‑150 (MQ=255)

                                                  tAGTTCATTTCAAGGGCGAAGTAGTCCCGCGCATTTTTAAAGCAAATATTTTCCACCACCCCGCCCAGCAGGTTGATGCCCGCCGGTGCTGCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTg                                                                                              <  1:19892/149‑1 (MQ=255)

                                                  tAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGa                                                                                             >  2:389859/1‑150 (MQ=255)

                                                   agttcagttaAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACg                                                                                           <  2:30980/151‑1 (MQ=255)

                                                         gttcaATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGgtgt                                                                                      >  1:262596/1‑150 (MQ=255)

                                                                ggCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCCCCATCTCGCCCGGCAGGTTGATGTCCCCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAgg                                                                              <  2:757419/151‑1 (MQ=255)

                                                                    aaGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGc                                                                          >  2:28925/1‑151 (MQ=255)

                                                                        agtCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTa                                                                                                    >  2:458023/1‑121 (MQ=255)

                                                                                    ttttAAACAAAATATTTTTCCCCATCTCCCCCAGCAGTTTGATGTCCGCCGGTGCTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTgtcagtca                                                           <  1:553620/148‑1 (MQ=255)

                                                                                     gTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCCGTCAGc                                                         >  1:737031/1‑151 (MQ=255)

                                                                                     gTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGc                                                         >  2:420389/1‑151 (MQ=255)

                                                                                        aaaGCAAATATTTTTCCCCATCTCGCCCAGCGGGTTGATGTCCGCGGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATa                                                      <  2:2225/151‑1 (MQ=255)

                                                                                        aaaGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCAt                                                       >  2:42586/1‑150 (MQ=255)

                                                                                         aaGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATAc                                                     >  1:618308/1‑151 (MQ=255)

                                                                                          agaaaaTCTTTTCCCCCATCCCGCCCAGCGGGTTGAGCCCCGCGGGTGCTTCCCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATa                                                      <  1:160596/146‑1 (MQ=255)

                                                                                           gCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCg                                                   >  2:567545/1‑151 (MQ=255)

                                                                                             aaaTATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAc                                                 <  2:639023/151‑1 (MQ=255)

                                                                                              aaTATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAc                                                 >  2:17524/1‑150 (MQ=255)

                                                                                                    tttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCgg                                          >  2:71507/1‑151 (MQ=255)

                                                                                                    tttCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCgg                                          >  1:416441/1‑151 (MQ=255)

                                                                                                        aCCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTc                                       >  2:450194/1‑150 (MQ=255)

                                                                                                         ccATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGAAAAAGCGGCTc                                       >  2:605014/1‑149 (MQ=255)

                                                                                                          cATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagc                                    >  2:294105/1‑151 (MQ=255)

                                                                                                           aTCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagc                                    >  2:760590/1‑150 (MQ=255)

                                                                                                            tctcGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTcagc                                    >  1:749764/1‑149 (MQ=255)

                                                                                                               cGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGAcc                               >  1:231253/1‑151 (MQ=255)

                                                                                                               cGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGAc                                <  2:20622/150‑1 (MQ=255)

                                                                                                                        ggTTGATGTCCGCGGTTGCTCCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTgcg                       <  1:398111/150‑1 (MQ=255)

                                                                                                                         gTTGATGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGcc                     >  2:453398/1‑151 (MQ=255)

                                                                                                                              tGTCCGCCGGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGtt                 <  2:392928/150‑1 (MQ=255)

                                                                                                                                 ccgccgGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGt                  <  1:453347/146‑1 (MQ=255)

                                                                                                                                   gccgGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTc            >  1:548133/1‑150 (MQ=255)

                                                                                                                                     cgGTGCTTCGCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATc         >  1:599434/1‑151 (MQ=255)

                                                                                                                                      gttGCTCCCCCCGCCTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATc         <  1:492758/148‑1 (MQ=255)

                                                                                                                                             cgcccgccTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGtt  <  1:417535/150‑1 (MQ=255)

ACGTTATCCAGCGCATGGATCTTGATGTATTGCATATCAACCCCAGACCTTAGTTCAGTTCAATGGCGAAGTAGTCACGCGCATTGTTAAAGCAAATATTTTTCACCATCTCGCCCAGCAGGTTGATGTCCGCCGGTGCTTCGCCCGCTTCCACCCAGCGACCGATCATCTGGCACAGAATGCGGCGGAAGTATTCGTGACGGGTGTATGACAGGAAGCTACGGCTGTCAGTCAGCATACCGACAAAGCGGCTCAGCAGACCGAGCTGCGCCAGTTGGGTCATCTGACGTT  >  NC_000913/3243280‑3243570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A