Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,288,276	A→G	N63D (AAT→GAT)	yraI →	putative periplasmic pilin chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,288,276	0	A	G	100.0%	132.4 / NA	35	N63D (AAT→GAT)	yraI	putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/20); total (15/20)

TGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATAATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTAATGTTCGT  >  NC_000913/3288129‑3288422

tgtAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAgatgat                                                                                                                                                  >  2:409548/1‑150 (MQ=255)

          gTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGt                                                                                                                                         <  1:70534/149‑1 (MQ=255)

           tATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTaa                                                                                                                                       >  1:545775/1‑150 (MQ=255)

                 ccAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGt                                                                                                                                <  1:105762/151‑1 (MQ=255)

                   aGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTg                                                                                                                               <  2:715262/150‑1 (MQ=255)

                       gcTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGcccc                                                                                                                           >  1:586605/1‑150 (MQ=255)

                                aggaATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTtattat                                                                                                                  >  1:479104/1‑150 (MQ=255)

                                     tCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAAcccc                                                                                                            >  1:573127/1‑151 (MQ=255)

                                       gTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCAc                                                                                                          >  1:233270/1‑151 (MQ=255)

                                            aCAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCt                                                                                                      <  2:880356/150‑1 (MQ=255)

                                            aCAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCt                                                                                                      <  1:470756/150‑1 (MQ=255)

                                               gCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTAttt                                                                                                  >  2:576671/1‑151 (MQ=255)

                                                   aCGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTc                                                                                              <  1:220162/151‑1 (MQ=255)

                                                                atgatgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTgg                                                                                    <  1:611065/148‑1 (MQ=255)

                                                                atgatgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTg                                                                                     <  1:73476/147‑1 (MQ=255)

                                                                atgatgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGc                                                                                   <  1:447078/149‑1 (MQ=255)

                                                                atgatgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGc                                                                                   <  1:347320/149‑1 (MQ=255)

                                                                atgatgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCg                                                                                  <  1:377545/150‑1 (MQ=255)

                                                                  gatgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGAt                                                                                <  1:631038/150‑1 (MQ=255)

                                                                           cGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCAc                                                                      <  2:527561/151‑1 (MQ=255)

                                                                                  agGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAat                                                               <  1:503759/151‑1 (MQ=255)

                                                                                  agGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAat                                                               >  1:62907/1‑151 (MQ=255)

                                                                                       gCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAattatt                                                           >  2:326564/1‑150 (MQ=255)

                                                                                                     aaacaaaGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAAc                                            <  1:140731/151‑1 (MQ=255)

                                                                                                         aaaGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAAcctgc                                        <  1:579735/151‑1 (MQ=255)

                                                                                                             gcgcAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAAcctgcctg                                     >  1:603798/1‑150 (MQ=255)

                                                                                                              cgcAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGaa                                   <  2:612110/151‑1 (MQ=255)

                                                                                                               gcAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGaa                                   >  2:395417/1‑150 (MQ=255)

                                                                                                                 aGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAAt                                >  2:219285/1‑151 (MQ=255)

                                                                                                                  gAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAAt                                >  1:610033/1‑150 (MQ=255)

                                                                                                                     aCGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTTATAACCTGCCTGAAAATaa                              <  1:331002/149‑1 (MQ=255)

                                                                                                                     aCGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATaa                              >  1:586383/1‑149 (MQ=255)

                                                                                                                           tATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTc                        <  1:298301/149‑1 (MQ=255)

                                                                                                                                      aTCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACAtt           <  2:479057/151‑1 (MQ=255)

                                                                                                                                        cATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTa          >  1:72620/1‑150 (MQ=255)

                                                                                                                                               aGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTAATGTTCGt  >  1:93157/1‑151 (MQ=255)

TGTAGCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATAATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTACATTAATGTTCGT  >  NC_000913/3288129‑3288422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A