Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,288,276	A→G	N63D (AAT→GAT)	yraI →	putative periplasmic pilin chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,288,276	0	A	G	100.0%	135.0 / NA	36	N63D (AAT→GAT)	yraI	putative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/20); total (16/20)

GCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATAATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTAC  >  NC_000913/3288133‑3288410

gCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGa                                                                                                                                 <  2:241419/151‑1 (MQ=255)

 cTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGatatcgct                                                                                                                                >  1:48327/1‑147 (MQ=255)

      gTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTaa                                                                                                                           <  2:33379/151‑1 (MQ=255)

            gCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCg                                                                                                                     <  2:306261/151‑1 (MQ=255)

             ccAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGt                                                                                                                    >  1:250255/1‑151 (MQ=255)

              cAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTg                                                                                                                   <  1:81523/151‑1 (MQ=255)

                       gCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATtta                                                                                                          <  1:240223/151‑1 (MQ=255)

                               aaTCGTTTTACAGCGAACGCGAGTGATCTATGATGCCTGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCTAACATGGGTAGATGATATAGATGGTAAAAGCCGTGCACCATTTATTATATcc                                                                                                  <  1:766417/151‑1 (MQ=255)

                               aaTCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAAcc                                                                                                  <  2:371857/151‑1 (MQ=255)

                                  cGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAAcccc                                                                                                <  1:703820/150‑1 (MQ=255)

                                  cGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAAcccc                                                                                                <  2:263007/150‑1 (MQ=255)

                                   gTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCAc                                                                                              <  2:233983/151‑1 (MQ=255)

                                     tttACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCAcc                                                                                             <  1:90413/150‑1 (MQ=255)

                                     tttACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCg                                                                                            >  2:308591/1‑151 (MQ=255)

                                        aCAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTa                                                                                         <  2:237279/151‑1 (MQ=255)

                                         cAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTAt                                                                                        <  1:388141/151‑1 (MQ=255)

                                             gAACGCGAGGGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCg                                                                                    >  2:384029/1‑151 (MQ=255)

                                                    aGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAg                                                                             <  2:594069/151‑1 (MQ=255)

                                                      tGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGc                                                                           >  2:625682/1‑151 (MQ=255)

                                                       gATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCt                                                                          >  2:770103/1‑151 (MQ=255)

                                                           tATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCg                                                                      >  2:375959/1‑151 (MQ=255)

                                                               atgCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATg                                                                   >  1:742406/1‑150 (MQ=255)

                                                                       cGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACtcatca                                                           <  2:406252/150‑1 (MQ=255)

                                                                          aaaGAGGCTGCGTTACCTGTCGCACACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCAc                                                          >  2:492462/1‑148 (MQ=255)

                                                                            agagGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCg                                                      >  2:575154/1‑150 (MQ=255)

                                                                                  tGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAattatt                                               >  1:751976/1‑151 (MQ=255)

                                                                                        aCCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACa                                         <  2:137889/151‑1 (MQ=255)

                                                                                            gTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTg                                     <  1:201933/151‑1 (MQ=255)

                                                                                                              gAAACGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAAt                    >  1:205150/1‑150 (MQ=255)

                                                                                                                aaCGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATaa                  >  2:426843/1‑150 (MQ=255)

                                                                                                                 aCGCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAag                <  2:631603/151‑1 (MQ=255)

                                                                                                                   gCCTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAagag              <  2:411082/151‑1 (MQ=255)

                                                                                                                     cTTATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTc            >  1:42879/1‑151 (MQ=255)

                                                                                                                      ttATTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCg           >  1:581244/1‑151 (MQ=255)

                                                                                                                        aTTTACTGCAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCt         <  1:318294/151‑1 (MQ=255)

                                                                                                                                cAATCATGGGTAGATGATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTAc  >  1:158828/1‑150 (MQ=255)

GCTTCTGTATTGGCCAGGCGCTTGCAGGAGGAATCGTTTTACAGCGAACGCGAGTGATCTATGATGCCAGCCGCAAAGAGGCTGCGTTACCTGTCGCAAACAAAGGCGCAGAAACGCCTTATTTACTGCAATCATGGGTAGATAATATAGATGGTAAAAGCCGTGCCCCATTTATTATAACCCCACCGCTATTTCGTCTTGAGGCTGGCGATGACTCATCACTGCGAATTATTAAAACAGCTGATAACCTGCCTGAAAATAAAGAGTCGCTGTTCTAC  >  NC_000913/3288133‑3288410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A