Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,260,849 G→A intergenic (‑46/+79) dauA ← / ← prs C4‑dicarboxylic acid transporter/phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,260,8490GA98.0% 149.9 / ‑5.2 49intergenic (‑46/+79)dauA/prsC4‑dicarboxylic acid transporter/phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (26/22);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

CAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATAC  >  NC_000913/1260701‑1260995
                                                                                                                                                    |                                                                                                                                                  
cAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGt                                                                                                                                                  <  1:649755/151‑1 (MQ=255)
  aTCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTg                                                                                                                                                 >  1:606453/1‑150 (MQ=255)
      ggTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAagg                                                                                                                                             >  1:512668/1‑150 (MQ=255)
          aCGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCa                                                                                                                                        <  1:104709/151‑1 (MQ=255)
            gggTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATa                                                                                                                                      <  1:443817/151‑1 (MQ=255)
             ggTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAg                                                                                                                                     <  1:474249/151‑1 (MQ=255)
                   ccGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCAt                                                                                                                                >  1:7751/1‑150 (MQ=255)
                              tataTTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGatat                                                                                                                    >  2:514814/1‑151 (MQ=255)
                                   ttttCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACa                                                                                                                <  2:678690/150‑1 (MQ=255)
                                   ttttCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCACATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACa                                                                                                                <  2:256097/150‑1 (MQ=255)
                                    tttctttcCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTAc                                                                                                                 >  1:625196/1‑148 (MQ=255)
                                     ttctttcCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGAc                                                                                                             >  2:228135/1‑151 (MQ=255)
                                        tttcCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACaa                                                                                                           >  2:145866/1‑150 (MQ=255)
                                           ccAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCGTAGGTCATACAAATGGATATTACAGACaaaaa                                                                                                        <  2:462841/150‑1 (MQ=255)
                                                atgCGTTGATCAGAGCGCGGAAAGGCATCACATGTCAGAATAATATTTTGTTCACAATAATGTTTCAACCGTGAGCGCAACAGCTGTGAACTAAATGGCAAGTGAAGGGGGCATAGGTCATACAAATGTATACTTCAGGCAAGAAAACccg                                                                                                  <  1:38628/149‑1 (MQ=255)
                                                aaGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACccg                                                                                                  >  1:278025/1‑151 (MQ=255)
                                                 aGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACccgc                                                                                                 >  1:32776/1‑151 (MQ=255)
                                                     tCGATCAGAGCGCGGAAAGGCATCACATGTGAGAAAAATATTTTGTTCAAAATAATGTTTCATCCGTGAGCGCATCATCTGTAAACTAAATGGAAAGTGAAGGAGGCATAGGTCATACAAAGGGATATTACAGAAAAAAAACCccgccg                                                                                               <  1:533944/149‑1 (MQ=255)
                                                       gATCAGAGCGCGGAAAGGCATCACATGTGAGAAAAATATTTTGTTCAAAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATGGGTCATACAAATGGATATTACAGACAAAAAACCCCGCCGCAg                                                                                            <  1:140687/150‑1 (MQ=255)
                                                               cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCttt                                                                                   >  1:261507/1‑151 (MQ=255)
                                                               cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCttt                                                                                   >  1:178201/1‑151 (MQ=255)
                                                                 cgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTg                                                                                  >  2:607905/1‑150 (MQ=255)
                                                                  ggAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTg                                                                                  >  1:580125/1‑149 (MQ=255)
                                                                    aaaGGCATCACATGTGAGAAAAATATTTTGTTCAAAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACAAAAGGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGc                                                                               <  1:102928/150‑1 (MQ=255)
                                                                             acaTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAAGGGCAAGTGAAGGAGGCATAGGTCATAAAAATGGATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAt                                                                     <  2:42649/151‑1 (MQ=255)
                                                                              caTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                    >  2:453898/1‑151 (MQ=255)
                                                                                     ggAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTc                                                                        >  1:25727/1‑140 (MQ=255)
                                                                                       aaaaTATTTTGTTCACAATAATGTTTCACCCGTGAGCGCATCATCTGTCAACTAAATGGAAAGGGAAGGAGGCATAGGTCATACAAAGGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTc                                                                        <  2:25758/138‑1 (MQ=255)
                                                                                       aaaaTATTTTGTTCAAAATAAGGTTTCACCAGGGAGCCCATCATCTTTCAACAAAATGGAAAGTAAAGGAGGCAAGGGTCATACAAATGGATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCg                                                            <  1:276447/150‑1 (MQ=255)
                                                                                             ttttGTACAAAAAAATGTTTCATCCGTGAGCAAATCATCTGTCAACTAAAGGAAAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATg                                                      <  1:521981/150‑1 (MQ=255)
                                                                                                  ttCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                  >  1:455786/1‑149 (MQ=255)
                                                                                                  ttCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATAAAAATGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                  <  2:512720/149‑1 (MQ=255)
                                                                                                   tCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                  >  2:218991/1‑148 (MQ=255)
                                                                                                     acaATAATGTTTCTTCGGTGAGCGGATCATCTGTCAACTAAATGGCAAGTGAAGGAGGAATGGGTCATAAAAATGGATATTAAAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCagag                                                <  2:429321/148‑1 (MQ=255)
                                                                                                       aataatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCg                                            >  2:534212/1‑150 (MQ=255)
                                                                                                               ttCACCGGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAGGGAGGCATAGGTAATACAAAGGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCg                                    <  1:2631/150‑1 (MQ=255)
                                                                                                                tCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAATAAACCCGCCGCAGCGCTTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATGGATTCTTCGtt                                  >  1:314308/1‑151 (MQ=255)
                                                                                                                   tCCGTGAGCGCATCATCTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCt                               >  2:541081/1‑151 (MQ=255)
                                                                                                                   cccGTGAGCGAATCATCTGTCAACTAAAGAGAAGATGAGGGAGGCATAGGTCATACAAAGGGATATTAAAGACAAAAAAGCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCt                               <  1:16016/150‑1 (MQ=255)
                                                                                                                               tcatcTGTCAACAAATTGGCAAGTGAGGGAGCCATAGGTCATACAAGTGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacgac                       <  2:444581/147‑1 (MQ=255)
                                                                                                                                 atcTGTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCg                 >  1:160109/1‑151 (MQ=255)
                                                                                                                                     gTCAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCt               <  2:367446/149‑1 (MQ=255)
                                                                                                                                       cAACTAAATGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCgg           >  2:47832/1‑151 (MQ=255)
                                                                                                                                        aaCTAAATGGAAAGTGAGGGAGGCATAGGTCATACAAAGGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGc          <  1:181265/151‑1 (MQ=255)
                                                                                                                                        aaCTAAAGGGAAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCg            <  1:607848/149‑1 (MQ=255)
                                                                                                                                              aTGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt    >  2:244051/1‑151 (MQ=255)
                                                                                                                                               tGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt    >  2:54030/1‑150 (MQ=255)
                                                                                                                                               tGGCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt    >  2:710076/1‑150 (MQ=255)
                                                                                                                                                ggCAAGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATAc  >  2:261825/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATAC  >  NC_000913/1260701‑1260995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A