Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,363,151	T→C	G115G (GGT→GGC)	yhcD →	putative outer membrane fimbrial subunit usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,363,151	0	T	C	100.0%	182.4 / NA	53	G115G (GGT→GGC)	yhcD	putative outer membrane fimbrial subunit usher protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (36/17); total (36/17)

AATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGAGC  >  NC_000913/3363005‑3363289

aaTGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCAcc                                                                                                                                         >  2:770225/1‑150 (MQ=255)

aaTGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACca                                                                                                                                        <  2:71020/151‑1 (MQ=255)

     cGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATc                                                                                                                                   >  2:522621/1‑151 (MQ=255)

      gAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATc                                                                                                                                   >  2:694090/1‑150 (MQ=255)

      gAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATc                                                                                                                                   >  2:64477/1‑150 (MQ=255)

          aaaaaGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACAcc                                                                                                                              >  2:493710/1‑151 (MQ=255)

             aaGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCAtt                                                                                                                           >  1:69710/1‑151 (MQ=255)

                 cAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCt                                                                                                                       >  2:769495/1‑151 (MQ=255)

                  aaCATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTa                                                                                                                      <  2:190592/151‑1 (MQ=255)

                   aCATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTaa                                                                                                                     <  1:549335/151‑1 (MQ=255)

                   aCATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTa                                                                                                                      >  1:515225/1‑150 (MQ=255)

                           aCGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGa                                                                                                               >  1:458184/1‑149 (MQ=255)

                             gcgcTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCg                                                                                                           <  2:268587/151‑1 (MQ=255)

                                cTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGtt                                                                                                         <  2:661508/150‑1 (MQ=255)

                                  ttGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCCCACCATTACCTAACCAGGAAGCGTTAg                                                                                                       <  2:295194/150‑1 (MQ=255)

                                   tgatgaAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGa                                                                                                      <  2:11161/150‑1 (MQ=255)

                                     atgaAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGatat                                                                                                   >  1:727481/1‑151 (MQ=255)

                                     atgaAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGatat                                                                                                   >  1:62777/1‑151 (MQ=255)

                                        aacaTGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGAtattat                                                                                                >  1:744802/4‑151 (MQ=255)

                                              gCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTc                                                                                          >  2:625238/1‑151 (MQ=255)

                                                gACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTcac                                                                                        >  1:562243/1‑151 (MQ=255)

                                                       gTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGc                                                                                 <  2:187340/151‑1 (MQ=255)

                                                        tCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCg                                                                                >  2:752788/1‑151 (MQ=255)

                                                        tCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCg                                                                                >  2:326932/1‑151 (MQ=255)

                                                          ttGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGAt                                                                              >  1:808493/1‑151 (MQ=255)

                                                          ttGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGAt                                                                              >  1:46100/1‑151 (MQ=255)

                                                             aTCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCAt                                                                           >  2:735626/1‑151 (MQ=255)

                                                              tCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCAtt                                                                          >  2:683072/1‑151 (MQ=255)

                                                                aGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTcc                                                                        <  1:194720/151‑1 (MQ=255)

                                                                       tCTGCAACAAGCTGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAgg                                                                  <  2:385997/150‑1 (MQ=255)

                                                                       tCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAggg                                                                 >  1:437616/1‑151 (MQ=255)

                                                                        cTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAggg                                                                 >  2:220948/1‑150 (MQ=255)

                                                                            aacaaGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTgg                                                             >  1:260044/1‑150 (MQ=255)

                                                                             acaaGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAt                                                           >  1:748361/1‑151 (MQ=255)

                                                                                aGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGAtctc                                                        >  1:393468/1‑151 (MQ=255)

                                                                                  ccGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCACATAGGGTTGGATCTca                                                       >  1:214092/1‑150 (MQ=255)

                                                                                   cGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTcac                                                      >  1:838687/1‑150 (MQ=255)

                                                                                          aaaaTTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACgc                                                >  1:139132/1‑149 (MQ=255)

                                                                                                ccTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACtg                                        >  2:811808/1‑151 (MQ=255)

                                                                                                ccTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACt                                         <  1:522583/150‑1 (MQ=255)

                                                                                                   tCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACtggt                                      <  2:30057/150‑1 (MQ=255)

                                                                                                         gaaAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAAc                                 >  1:816933/1‑149 (MQ=255)

                                                                                                            aaaaaTGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAgctg                            >  1:237873/1‑151 (MQ=255)

                                                                                                             aaaaTGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAgctgc                           >  2:255520/1‑151 (MQ=255)

                                                                                                              aaaTGGCTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAgctgcg                          <  1:617020/151‑1 (MQ=255)

                                                                                                                    cTGTTACGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCgctgct                     >  2:822310/1‑150 (MQ=255)

                                                                                                                         aCGACTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAacta               <  1:694047/151‑1 (MQ=255)

                                                                                                                            aCTATATATTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTActc            >  2:465606/1‑151 (MQ=255)

                                                                                                                              tatataTTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTActctc          >  2:688041/1‑151 (MQ=255)

                                                                                                                               atatatTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTActctct         <  2:191236/151‑1 (MQ=255)

                                                                                                                                 atatTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCtg        >  2:748538/1‑150 (MQ=255)

                                                                                                                                   atTGTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCtgat      <  2:409391/150‑1 (MQ=255)

                                                                                                                                      gTCCTACCCGGGCACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGagc  <  2:243811/151‑1 (MQ=255)

AATGGCGAAAAAAAAGGCAACATTGCTACGCGCTTTGATGAAAATGGCGACATTTGTCTTGATCAGGCATTTCTGCAACAAGCCGGTTTAAAAATTCCTTCTGAAGAAAAAAATGGCTGTTACGACTATATATTGTCCTACCCGGGTACCACAATCACACCATTACCTAACCAGGAAGCGTTAGATATTATCGTTTCACCACAGGCGATCATTCCCATAGGGTTGGATCTCACAAACGCAGCAACTGGTGGAACAGCTGCGCTGCTAAACTACTCTCTGATGAGC  >  NC_000913/3363005‑3363289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A