Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I2 R1	14	50.4	1586623	98.1%	1556477	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,550	T→G	W100G (TGG→GGG)	gabP →	gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,550	0	T	G	100.0%	120.4 / NA	35	W100G (TGG→GGG)	gabP	gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (16/19); total (16/19)

TATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACG  >  NC_000913/2794403‑2794697

tATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGGTACCCGTTCGTTTTCTAACTATGCCGATAAAGCCCTTGGTCGCTGGGGGGGGTATACCATCGGCCGGGTTTACtgggggg                                                                                                                                                  >  2:59201/1‑150 (MQ=255)

 aTCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACtggggg                                                                                                                                                   <  2:85647/149‑1 (MQ=255)

     gTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTg                                                                                                                                              <  2:462983/150‑1 (MQ=255)

      ttCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTTCCACGCCCGATACCGGGTCGTTTTTCACCCATGCCGATAAAGCCATTGGTCGCCGGGCGGGCTATACCATCGGCTGGGTGTACTGGGGGTTTTtgg                                                                                                                                            >  1:674573/1‑151 (MQ=255)

        cGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTTCACCTATGCCGATAAAGCCCTTGGTCGCTGGGCGGGCTATACCATCGGCTGGGTGTTCTGGGGGGTTTggg                                                                                                                                            >  1:471050/1‑149 (MQ=255)

          ccGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACt                                                                                                                                        <  2:768356/151‑1 (MQ=255)

                 aCTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTa                                                                                                                                   <  1:502483/149‑1 (MQ=255)

                              aTTATGCGGATGTTGGCGGAAATGGCGGCTGCCACGCCCGATACCGGGTCGTTTTTTACCCATTCCGATAAAGCCATTGGGCGCGGGGCGGGATATACCATCGGGTGGGTGTACTGGGGGGTTTGGGGAATGGGTATCCCGCCgggaacc                                                                                                                     >  2:203153/1‑145 (MQ=255)

                                            ggCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCAt                                                                                                       <  2:98169/150‑1 (MQ=255)

                                                 aaaTGGCGGTGGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCt                                                                                                 <  2:266174/151‑1 (MQ=255)

                                                     ggCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCAc                                                                                             <  2:569563/151‑1 (MQ=255)

                                                           tGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATg                                                                                        <  2:676301/150‑1 (MQ=255)

                                                              cACGCCCGTTACCTGGTCGTTGTCCACCTATGCCGATAAAACCATTGGCCGCTGGGCGGGCTATACCATCGGATGGCTGAACTGGGGGTTTTGGGTACTGGGTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATggggt                                                                                    >  2:110674/1‑149 (MQ=255)

                                                                   ccGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTAGGCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAgg                                                                               <  2:43296/151‑1 (MQ=255)

                                                                    cGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGc                                                                              <  1:545955/151‑1 (MQ=255)

                                                                     gATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGc                                                                              >  2:182235/1‑150 (MQ=255)

                                                                         tcGGTTCGTTTTCCACCTATGCCGGTAAAGCCATCGCTCGCTAGGCGGCCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTAATGGGTTCCACGCATTc                                                                          >  2:583132/2‑150 (MQ=255)

                                                                                ggtttCCACCTATTCCGATAAATCCATTGGTCGCTGGGGGTGCTATACCATCGGCTGGTTGAACTAGGGGTTTTGGGGACCGGTTATCCCGCTCGAAGCAAACATTGCCGCCATGATACGGCACTCATGGGTTCCCGGCa                                                                             >  2:630740/3‑140 (MQ=255)

                                                                                gTTTTCCACCAATGCCGATACAGACATTGGTCCCTGGGCGGGCTATACCATCGGCTGGCTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGTTCCTTCATTCATGGGCTCCAGGCATTCCCATc                                                                     >  2:771817/1‑148 (MQ=255)

                                                                                  tttCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGtt                                                                >  2:163244/1‑151 (MQ=255)

                                                                                  tttCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGt                                                                 <  1:61075/150‑1 (MQ=255)

                                                                                      cACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTAttt                                                            <  2:547299/151‑1 (MQ=255)

                                                                                        ccTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTc                                                          <  1:128159/151‑1 (MQ=255)

                                                                                           aTGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTccc                                                        >  2:732386/1‑150 (MQ=255)

                                                                                                   aaaGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCAtt                                                >  1:16145/1‑150 (MQ=255)

                                                                                                     aGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCCCGTCATTAcc                                             >  2:700163/1‑151 (MQ=255)

                                                                                                            ggTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCt                                      <  1:343036/151‑1 (MQ=255)

                                                                                                                gCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAAc                                  >  2:135461/1‑151 (MQ=255)

                                                                                                                  tGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTg                                >  1:407863/1‑151 (MQ=255)

                                                                                                                    ggcgggcTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGc                              <  2:386283/151‑1 (MQ=255)

                                                                                                                     gcgggcTATCCCCTCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCCCTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACt                                 <  2:216507/147‑1 (MQ=255)

                                                                                                                               ccATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACtta                     <  1:365957/149‑1 (MQ=255)

                                                                                                                                     gCTGGTTGTACTGGGGGTTTTGGGTAATGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGc               <  2:550904/149‑1 (MQ=255)

                                                                                                                                        ggTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGtt            <  1:135496/149‑1 (MQ=255)

                                                                                                                                          ttGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTaaa         >  1:737935/1‑150 (MQ=255)

                                                                                                                                           tGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTaaaa        <  2:451419/150‑1 (MQ=255)

                                                                                                                                              aCTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACt     >  2:761756/1‑150 (MQ=255)

                                                                                                                                                tgggggTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACg  >  2:465753/1‑151 (MQ=255)

TATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACG  >  NC_000913/2794403‑2794697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A