Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F50 I2 R1
|
173 |
150.8 |
4954114 |
96.5% |
4780720 |
150.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,097,218 |
(G)7→8 |
intergenic (+389/+347) |
ycdU → / ← serX |
putative inner membrane protein/tRNA‑Ser |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,097,211 | 1 | . | G | 92.5%
| 72.0
/ 2.1
| 40 | intergenic (+382/+354) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (5/32); total (8/32) |
| Fisher's exact test for biased strand distribution p-value = 5.67e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑A‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGT > NC_000913/1097063‑1097306
|
ggCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑A‑gg < 4:1753343/151‑1 (MQ=33)
aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑A‑G‑T‑ggggg > 1:1535828/1‑151 (MQ=18)
ccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCgg‑g‑g‑g‑gggggggg > 4:14229/1‑150 (MQ=18)
ccGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑g‑gggggggg > 4:1688079/1‑150 (MQ=18)
cGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑A‑gggggggga < 1:1361400/151‑3 (MQ=18)
gCTCACTGCGTTCTCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑A‑gggggggg < 1:794405/149‑2 (MQ=18)
tCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑A‑ggggggggg > 1:1836921/1‑146 (MQ=18)
tCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑A‑gggggggg > 3:82171/1‑146 (MQ=18)
tCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑G‑G‑A‑ggggggggg > 1:418981/1‑146 (MQ=18)
cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGa < 4:688725/151‑1 (MQ=18)
cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGa < 2:114262/151‑1 (MQ=18)
cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑A‑ggggggggg > 1:936630/1‑145 (MQ=18)
cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑A‑G‑A‑gggggggg > 2:378884/1‑145 (MQ=18)
cGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACtcgc < 2:331454/151‑1 (MQ=18)
cGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑G‑G‑A‑ggggggggggtgaa > 4:151320/1‑140 (MQ=255)
gTTGACGGTCTGTCCAACTGGCTGTCGCCTGCTCTCGACCCCCAGACGGGGCTTCTGATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACtcgct < 1:629071/151‑1 (MQ=11)
cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGCCCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACtcgcttc < 1:659840/150‑1 (MQ=16)
aCGGTCTGCCCAACTGGCTTTCGACAGTTGCCGGCCCCCGCGCGGGGGTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACtcgcttc < 1:942972/149‑1 (MQ=11)
ccAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGc < 4:1721149/151‑1 (MQ=18)
cTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAG‑G‑G‑GGGGGGGGGTGACTCGCTTCGCTCGCCCTCCggg > 1:1253133/1‑146 (MQ=11)
tGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑G‑GAA‑AGGGGGGAGGTACTCCCTTCGCCCTCCCTGCGGGccg > 3:351450/1‑146 (MQ=11)
gTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGccc < 4:930913/150‑1 (MQ=18)
gTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCg < 4:698724/151‑1 (MQ=18)
gCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTc < 4:50584/151‑1 (MQ=18)
gTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCAc < 1:407566/149‑1 (MQ=18)
tGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGc < 4:774503/150‑1 (MQ=18)
gTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCg < 2:379508/150‑1 (MQ=18)
tCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGTTTTACTCCCTTCCCCCCCCCCGCGGGCAGCCCCCCCCCTTCGtt > 2:45709/1‑151 (MQ=11)
gACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑TGG‑G‑GGGGGGGGTTGGCTCGCCTTCCCCGCCCCGCGGGCAGccccccccctggggt > 1:820523/1‑137 (MQ=11)
ccccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACgg < 4:287291/151‑1 (MQ=14)
cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCt < 1:1911087/151‑1 (MQ=14)
ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTg < 1:1302502/151‑1 (MQ=14)
cGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTTAATCGCTTCGCCCGCCCTGCGGGGGGCCCCCCCCCCGCGTTCACGgtccgtcc > 4:1769148/1‑151 (MQ=11)
ggggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCa < 1:398638/151‑1 (MQ=14)
ggggCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCa < 2:314879/151‑1 (MQ=14)
ggCTTCTCATCCCCCCGGTTTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATAATGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc < 1:814001/147‑1 (MQ=255)
tctcATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTg < 2:438297/149‑1 (MQ=14)
ccccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGGTTGACTCCCTTCGCTCGCCCCCCGGGGAACCCCCCCACTGCGGTTCCGGTCTTTCCAACCTGGTGTCg > 4:1226410/1‑150 (MQ=11)
ccccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCg < 1:1009478/150‑1 (MQ=14)
ccccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCa < 4:1350947/151‑1 (MQ=14)
ccGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGt < 1:735609/151‑1 (MQ=14)
cGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGtt < 2:372799/151‑1 (MQ=14)
cGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑A‑G‑AGGGGGGGGGGTGACTCCCTTCCCCCCCCCCCCGGGGACCCCCCCCCCTGCGGTTTCGGGCTGTCCAACAGGCTGTTGCCCGtt > 4:362525/1‑151 (MQ=2)
gtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGaa < 2:302888/151‑1 (MQ=14)
gtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGaa < 2:287451/151‑1 (MQ=14)
gtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGa < 4:990589/150‑1 (MQ=14)
gtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGAGTGAATCGCTTCGCTCCCCCTCCGGGGAGCCCCCTCCCCCCGGTCACGGTCTGTTCAACTGGCTGTCGCCCGtt > 4:938531/1‑145 (MQ=11)
gCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAc < 1:575760/150‑1 (MQ=14)
cAATACACGAAAAAAAAGCCCGTTCTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAc < 1:1167490/149‑1 (MQ=9)
cAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAAccc < 4:971934/151‑1 (MQ=14)
acacGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCgg < 1:1362678/150‑1 (MQ=14)
acacGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑AGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGt < 1:1166471/151‑1 (MQ=14)
|
GGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGG‑T‑G‑A‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGT > NC_000913/1097063‑1097306
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
