Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I2 R1	150	68.2	2132745	98.7%	2105019	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,218	(G)_7→8	intergenic (+389/+347)	ycdU → / ← serX	putative inner membrane protein/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,211	1	.	G	100.0%	12.7 / NA	8	intergenic (+382/+354)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (2/6); total (2/6)

GGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCC  >  NC_000913/1097063‑1097303

ggCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gg                                                                                            <  1:650576/151‑1 (MQ=33)

  cAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggg                                                                                          <  2:728921/151‑1 (MQ=33)

    gCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGATA‑gggggg                                                                                        >  1:463847/1‑151 (MQ=18)

       cGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCCACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTCCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg                                                                                           <  2:475958/145‑1 (MQ=18)

         cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggggggg                                                                                      >  1:228993/1‑147 (MQ=18)

           cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCggggg‑ggggggggggtg                                                                                  >  1:514235/1‑145 (MQ=255)

               gCGTTCTCGGTCTGTCCATCTGGTTGTCGCCAGTTGTCGACCCCAGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGAc                                                                                 <  2:791942/148‑1 (MQ=11)

               gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCAGGTGGGGGCGTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcg                                                                              <  2:481753/151‑1 (MQ=255)

                cGTTCACGGCCTGCCCATCTGGTTGTCGACAGTTGCCCACCCCAGCTGGGGGGTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggggggg                                                                                      <  2:45798/141‑2 (MQ=11)

                   tCACGGTCTGTCCAACTGGCTGTCGACAGTTCTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgctt                                                                           <  2:1057681/150‑1 (MQ=255)

                    cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑gggggggggatgaacc                                                                              >  1:131458/1‑136 (MQ=16)

                       ggTCTGTCCAACTGGCTGACGCGAGTTGTCGGCCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTtcgctc                                                                      <  1:727226/151‑1 (MQ=255)

                       ggTCTATCCAACTGGCGGTCGCCAGATGCCGCCCCCCGGTCGGGTTGTCTCGTCCACCCGGTTTGTGCAATACAAGAAAAAAAAGCCCGTACTTTCGGACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACtcgcttcg                                                                         <  2:421408/148‑1 (MQ=11)

                                             cAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCAc                                                <  1:692980/151‑1 (MQ=18)

                                                                                         gCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAACCCCCCCCCTCGCCCCGCGGGCAGCCCCCCCACTGCGTTCCCGGTCTGTCCCACTCGGCGTCGCCCCTTTTCGAAcc    >  1:434995/1‑151 (MQ=2)

                                                                                           aaTACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGAATCCCTTCGCCCCCCCCGCGGGCAGCCCCCTCCCTGTGTTCACGGTCTTTCCAACTGGGTGTCCCCCGTTGTCGGAcccc  >  1:207984/1‑151 (MQ=11)

GGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCC  >  NC_000913/1097063‑1097303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A