Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,366,223 A→G F184S (TTC→TCC)  dcuA ← C4‑dicarboxylate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,366,2230AG99.4% 511.8 / NA 154F184S (TTC→TCC) dcuAC4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (84/69);  total (84/70)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 158 total aligned reads displayed.

CCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGAAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAT  >  NC_000913/4366076‑4366369
                                                                                                                                                    |                                                                                                                                                  
cccAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCa‑gggagg                                                                                                                                                 <  1:1603703/151‑1 (MQ=255)
 ccAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCa‑gggagga                                                                                                                                                <  2:210941/151‑1 (MQ=255)
 ccAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCa‑gggagga                                                                                                                                                <  4:31154/151‑1 (MQ=255)
   agcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGAc                                                                                                                                               >  4:1450676/1‑150 (MQ=255)
    gcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACAt                                                                                                                                             <  1:1032621/151‑1 (MQ=255)
    gcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACAt                                                                                                                                             >  1:1604046/1‑151 (MQ=255)
     cagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATc                                                                                                                                            <  2:315430/151‑1 (MQ=255)
       ggAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCAc                                                                                                                                          >  2:262714/1‑151 (MQ=255)
          aCAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAg                                                                                                                                       <  2:419028/151‑1 (MQ=255)
              ccAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAAcc                                                                                                                                   <  4:798696/151‑1 (MQ=255)
              ccAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAAcc                                                                                                                                   <  4:743274/151‑1 (MQ=255)
              ccAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAAcc                                                                                                                                   <  4:54038/151‑1 (MQ=255)
              ccAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAAcc                                                                                                                                   <  1:1870359/151‑1 (MQ=255)
                aGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGc                                                                                                                                 >  4:175035/1‑151 (MQ=255)
                aGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGc                                                                                                                                 >  4:1026506/1‑151 (MQ=255)
                       cgTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAgg                                                                                                                          >  4:404446/1‑151 (MQ=255)
                       cgTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAgg                                                                                                                          >  4:1201018/1‑151 (MQ=255)
                        gTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAACTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAggg                                                                                                                         >  4:835908/1‑151 (MQ=255)
                        gTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAggg                                                                                                                         >  1:1467833/1‑151 (MQ=255)
                        gTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAggg                                                                                                                         >  1:838078/1‑151 (MQ=255)
                           tttGCCCCGTATTTGATTCCAATCTGTTTTTCCCCGCGCATTTCACCCAGGCCCTCTTCCAGACGCTTGGGATAAATCGGACCGTCAGAGAGTTTGGAGTTGAAAAGCATAGTGACCA‑GGGAGGACATCACAAGAACCGCCAGCAGGGTg                                                                                                                       <  1:1145613/150‑1 (MQ=255)
                           tttGCACCGGATTTGATTTCAATCTGCTTTTCCCCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCG‑GGGAGGACATCCCCAGAACCGCCAGCAGGGTg                                                                                                                       <  1:1370024/150‑1 (MQ=255)
                             tGCACCGGATTTGTTTTCAATCTGTTTTTCCCCGCGCAGTTCAACCAGGCCCTCTCCCAGACGTTTGCGATAAATCGGATCGTCAGAAAGTTTGGAGTTAAACAGAATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGa                                                                                                                     <  1:800708/150‑1 (MQ=255)
                                    gatttgatttAAATCTGCTTTTCCCCGCGCAGTTCAACCAGGCCCTCTTCCAGCCGCTTGCGATAAAGCGGATCGTCAGAGAGTTGGGAGTTGACCAGCATAGTGACCA‑GGGAGGACACCCCCAAAACCGCCAGCAGGGTGGACGGGATGa                                                                                                             <  1:267144/151‑1 (MQ=255)
                                        tgatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCAc                                                                                                         >  1:523725/1‑151 (MQ=255)
                                           tttCAATCTGCTTTCCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACgg                                                                                                       <  1:1010064/150‑1 (MQ=255)
                                            ttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACgg                                                                                                       >  4:789199/1‑149 (MQ=255)
                                              caatcgctttttCACCGGCCATTCCAACCCGGCCCTCTGCCAGGCCCTAGCCATAAACCGCATCGTCAGAATGTGTGGAGGAGAGCAGCATA‑ACACCAGGGAAGGACAGCACCAAAACCGCCAGCAGGGTGGACGGGATGACCACgg                                                                                                       <  1:284093/139‑1 (MQ=255)
                                               aaTCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  1:1912081/1‑151 (MQ=255)
                                                 tCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  4:1650539/1‑149 (MQ=255)
                                                 tCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  1:217857/1‑149 (MQ=255)
                                                    gCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATg                                                                                             >  1:1903424/1‑151 (MQ=255)
                                                    gCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATg                                                                                             >  3:350449/1‑151 (MQ=255)
                                                     cTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATgg                                                                                            >  4:208403/1‑151 (MQ=255)
                                                     cTTTTCACCGCGAAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGATTTGAACAGAATAGGGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATg                                                                                             <  3:538497/150‑1 (MQ=255)
                                                      ttttCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCa                                                                                                 <  1:743717/145‑1 (MQ=255)
                                                      ttttCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACACCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATg                                                                                             <  4:1914776/149‑1 (MQ=255)
                                                        ttCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAg                                                                                          <  2:455937/150‑1 (MQ=255)
                                                         tctccccgcAGTTCAACCAGGCCCTCGTCGAGTCACTGGCGAAAAAGCGAATCATCGGAGATTTTGGAGTGAAAAGGCTTAGCAACGG‑GGGAGGCCACCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGATCa                                                                                                 <  1:1897518/136‑1 (MQ=255)
                                                          cACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGt                                                                                        >  1:241819/1‑150 (MQ=255)
                                                          cACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGAAAAATCGGATCGTCAGAGAGTTGGGAGTGGAACAGCATAGTGACCA‑GGGAGGACATCACAAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAgg                                                                                         <  3:440257/149‑1 (MQ=255)
                                                          cACCGCGCAGTTCAACCAGGCCCTCTCCCAGCCGCTTGCGATAAATCGGATCGTCAGAGAGTTGGGGGTAGAACAGAATAGAGACCG‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGt                                                                                        <  1:982/150‑1 (MQ=255)
                                                           aCCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGt                                                                                        >  4:1310084/1‑149 (MQ=255)
                                                           aCCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGt                                                                                        >  4:13809/1‑149 (MQ=255)
                                                            ccGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGc                                                                                     >  4:45453/1‑151 (MQ=255)
                                                             cgcgCAGTTAAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGAATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCt                                                                                    <  2:106053/151‑1 (MQ=255)
                                                              gcgcAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTg                                                                                   >  4:1347575/1‑151 (MQ=255)
                                                              ccgcAGTTCTACCAGGCCCTCTTCCTGACGATTGCGATAACTCGGATCGTGAGAGAGATTGGAGTTCAGCAGAATAAAGATCA‑ACGAGGACATCACCAGAACCGCCAGCAGGGTGTACGGGAAGACCACGGAGAGCAGATGGACGTAGCt                                                                                    >  3:61750/2‑150 (MQ=255)
                                                               cgcAGTTCACCCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTg                                                                                   <  3:272524/150‑1 (MQ=255)
                                                                gcAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGAt                                                                                 >  1:567951/1‑151 (MQ=255)
                                                                     tCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCAt                                                                            >  2:385159/1‑151 (MQ=255)
                                                                      cAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATg                                                                           >  4:1274683/1‑151 (MQ=255)
                                                                       acccAGGCCCTCTCCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACAAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCAt                                                                            <  1:1784526/147‑1 (MQ=255)
                                                                       acccAGGCCCTCTCCAAGCCGCTTGCGAAAATTCGGTTCGCAAGAGAGTTTGAAGTTTAACGGCATAGTGACCA‑GGGAGGACCTCCCAAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGa                                                                          <  1:726070/149‑1 (MQ=255)
                                                                       aaCCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATg                                                                           <  2:167621/150‑1 (MQ=255)
                                                                       aaCCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATg                                                                           >  1:50323/1‑150 (MQ=255)
                                                                          cAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCt                                                                       >  4:249918/1‑151 (MQ=255)
                                                                            ggCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACAAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTc                                                                     <  2:527497/151‑1 (MQ=255)
                                                                             gCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTcc                                                                    >  1:1000639/1‑151 (MQ=255)
                                                                               ccTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGTGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCAt                                                                  >  1:228461/1‑151 (MQ=255)
                                                                                   ttCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACg                                                              >  4:1578769/1‑151 (MQ=255)
                                                                                    tCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACgg                                                             >  2:194622/1‑151 (MQ=255)
                                                                                    cccAGACGCTTGCGATAAATCGGATCGTTAGAGAGTTTGGATTTGAACAGCATAGTGACCA‑GGGAGGACATCACGATAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACgg                                                             <  4:1167931/150‑1 (MQ=255)
                                                                                     ccAGACGCTTGCGAAAAATCGGATCGTCAGAAAGTTGGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGa                                                            <  4:1774735/151‑1 (MQ=255)
                                                                                       aGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGa                                                            >  1:1766767/1‑149 (MQ=255)
                                                                                           gCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACAt                                                      >  3:311796/1‑151 (MQ=255)
                                                                                            cTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACAt                                                      >  4:1123468/1‑150 (MQ=255)
                                                                                             ttGCGATAAATCGGGTCGCAAGAGAGTTTGGAATTGAAAGAGATAGTGGCCA‑GGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAg                                                          <  2:153583/145‑1 (MQ=255)
                                                                                             ttGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGt                                                    >  4:700680/1‑151 (MQ=255)
                                                                                               gCGATAAATCGGATCGTCAGAGAGTTGGGAGTTGAACAGCATAGGGACCA‑GGGAGGACATCCCCAGAGCCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATg                                                     <  3:141656/148‑1 (MQ=255)
                                                                                                cGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTaa                                                  >  1:869443/1‑150 (MQ=255)
                                                                                                    aaaTCGGATCGTCAGAGATTTGGGAGTTGAACAGCATAGGGCCCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCa                                              <  4:843084/150‑1 (MQ=255)
                                                                                                    aaaGCGGATCGTCAGAGAGTTTGAAGTTAAACAGCATAGTGACGG‑GGGAGAACATCTCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCa                                              <  4:852614/150‑1 (MQ=255)
                                                                                                     aagcGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACt                                            <  4:1685295/148‑1 (MQ=255)
                                                                                                     aaTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACAAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACt                                            <  1:937471/151‑1 (MQ=255)
                                                                                                      aTCGGATCGTCAGAGATTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTg                                           <  1:1444747/151‑1 (MQ=255)
                                                                                                         ggATCGTCAGAGAGTTGGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTg                                           <  4:342282/148‑1 (MQ=255)
                                                                                                           aTCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCt                                      >  4:125331/1‑151 (MQ=255)
                                                                                                             cGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTg                                     >  4:1433557/1‑150 (MQ=255)
                                                                                                              gTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACACCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTgag                                   <  1:1703123/151‑1 (MQ=255)
                                                                                                                cAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTg                                     >  4:1411540/1‑147 (MQ=255)
                                                                                                                  gagagTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTg                               >  1:1047611/1‑151 (MQ=255)
                                                                                                                     agTTTGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCg                            >  4:230106/1‑151 (MQ=255)
                                                                                                                        ttGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGAt                          <  4:1567869/150‑1 (MQ=255)
                                                                                                                        ttGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATg                         >  1:1234211/1‑151 (MQ=255)
                                                                                                                         tGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATg                         <  4:101523/150‑1 (MQ=255)
                                                                                                                         tGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGc                        >  4:1679711/1‑151 (MQ=255)
                                                                                                                         tGGAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGc                        >  1:585354/1‑151 (MQ=255)
                                                                                                                         tGGAGTTGAACAGCAAAGTGCCCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATg                         <  4:584182/150‑1 (MQ=255)
                                                                                                                          ggAGTTGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGAt                          <  2:482835/148‑1 (MQ=255)
                                                                                                                              ttGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCATCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTg                    >  1:960087/1‑150 (MQ=255)
                                                                                                                               tGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGAt                  >  1:1661197/1‑151 (MQ=255)
                                                                                                                               tGAACAGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGAt                  >  4:630213/1‑151 (MQ=255)
                                                                                                                                    aGCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCa              >  1:275412/1‑150 (MQ=255)
                                                                                                                                     gCATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCa              <  4:1130903/149‑1 (MQ=255)
                                                                                                                                     gCATAGTGACCA‑GGGAGGACATCACAAGAACCGCCAGCAGGGTGGACGGGATGACCAAGGAGAGCAGATGGAGGTAGCTGATGCCATGACATTCCATCACGGACGACATGTAAACCACTGACGCTGAGATTGGCGATGCGGTGATCGCAAt            >  3:364914/1‑151 (MQ=255)
                                                                                                                                      cATAGTGACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATc           >  3:134287/1‑151 (MQ=255)
                                                                                                                                       aTAGTGACCA‑GGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATc           <  4:650661/150‑1 (MQ=255)
                                                                                                                                         agagACCA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTg         <  1:1798110/147‑1 (MQ=255)
                                                                                                                                               cA‑GGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAt  >  4:980172/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCA‑GGAAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAT  >  NC_000913/4366076‑4366369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A