Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I1 R1 144 141.8 4677362 93.6% 4378010 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,366,223 A→G F184S (TTC→TCC)  dcuA ← C4‑dicarboxylate antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,366,2230AG98.4% 402.0 / NA 125F184S (TTC→TCC) dcuAC4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (73/49);  total (74/51)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 128 total aligned reads displayed.

AGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAT  >  NC_000913/4366079‑4366369
                                                                                                                                                |                                                                                                                                                   
agcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACa                                                                                                                                               <  1:1688971/151‑1 (MQ=255)
  cagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATc                                                                                                                                             >  1:131840/1‑151 (MQ=255)
  cagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATc                                                                                                                                             >  1:1656275/1‑151 (MQ=255)
  cagGAACAGCCAGACGGACGGTTTTGCACCGGACTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATc                                                                                                                                             >  3:25641/1‑151 (MQ=255)
   agGAACAGCCAGACGGACGTTTTTGCACCGGATTTGAATTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCa                                                                                                                                            <  1:1308775/151‑1 (MQ=255)
    ggAACAGCCAGACGGACGTTTTTGCCCCGGATTTGATTTCAAACTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACTCTTGCGATAGATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATGGTGACCAGGGAGGACATCa                                                                                                                                            <  1:622579/150‑1 (MQ=255)
        cAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCAcc                                                                                                                                          >  3:108889/1‑148 (MQ=255)
        cAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCAcc                                                                                                                                          >  2:1602258/1‑148 (MQ=255)
         aGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑aa                                                                                                                                     <  1:274324/151‑1 (MQ=255)
         aGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑aa                                                                                                                                     <  2:372966/151‑1 (MQ=255)
          gCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AAc                                                                                                                                    >  1:1066033/1‑151 (MQ=255)
           ccAGACGGACGTTTTTGCACCGGGTTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AAcc                                                                                                                                   >  1:1497369/1‑151 (MQ=255)
             aGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGc                                                                                                                                 >  2:497972/1‑151 (MQ=255)
              gacggacgTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGcc                                                                                                                                >  2:1342916/1‑151 (MQ=255)
                cggacgTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGAAAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGcc                                                                                                                                >  2:660348/1‑149 (MQ=255)
                     gTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAggg                                                                                                                         >  2:1728981/1‑151 (MQ=255)
                     gTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAggg                                                                                                                         >  2:528313/1‑151 (MQ=255)
                      tttttGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCcagcag                                                                                                                           >  1:1103091/1‑148 (MQ=255)
                      ttttgGCACCGGATTTGATTTCAATGTCTTTTTCCCCGCGCAATCCAACCAGGCCCTCTCCCAGACGCTCGCAAAAAATCGTACTGCCGGAAAGTTTGGAGTGGAGCTGCATGGTGACGGGGAAGTAAATC‑CCAGCAAGCGCCAGCAgg                                                                                                                          <  1:1190397/149‑1 (MQ=255)
                       ttttGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGATTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGt                                                                                                                        <  4:70756/150‑1 (MQ=255)
                         ttGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGTGAGTTTGGAGCTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGCgg                                                                                                                      >  4:10950/1‑150 (MQ=255)
                            cACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACg                                                                                                                   <  4:463434/150‑1 (MQ=255)
                             aCCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACggg                                                                                                                 >  1:689280/1‑151 (MQ=255)
                                 gatttgatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATg                                                                                                              >  2:1060359/1‑150 (MQ=255)
                                     tgatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCAc                                                                                                         >  2:1537292/1‑151 (MQ=255)
                                      gatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACg                                                                                                        >  1:1652544/1‑151 (MQ=255)
                                          tCAATCTGTTTTTCACCGCGCAGTTCAACCGGGCCCTCTTCCAGAGCCTTGCGATAAATCGGACCGTCAGAGAGTTTGGAGTTGAACAGAAGAGTGACCAGGGGGGACATCACAAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGgag                                                                                                     <  1:969557/150‑1 (MQ=255)
                                            aaTCTGCTTTTCCCCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCTTCAGAGAGTTTGGAGTTGAACAGCATGGTGACCAGGGAGGACACCCCAAA‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  <  1:1653849/151‑1 (MQ=255)
                                             aTCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  2:803525/1‑150 (MQ=255)
                                              tCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  1:586520/1‑149 (MQ=255)
                                               cTGCTTTTCACCGCTCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  2:1529448/1‑148 (MQ=255)
                                               cTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  1:430958/1‑148 (MQ=255)
                                                tGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGCGGACGGGATGACCACGGAGAGc                                                                                                  >  1:813923/1‑147 (MQ=255)
                                                 gCTTTTCACCGCGCAGTTCAGCAAGGCCCTCTTCCAGACGCTTGGGACAAATCGGATGGTCAGAGAGTTTGGAGTGGACCAGCATAGTGACGAGGAAGGACACCACAAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGa                                                                                               <  4:389380/149‑1 (MQ=255)
                                                  cTTTCCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGAAAAATCGGATCGTCAGAGAGTTGGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATg                                                                                             <  4:583198/150‑1 (MQ=255)
                                                   ttttCCCCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCa                                                                                                 <  3:141545/145‑1 (MQ=255)
                                                   ttttCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACggg                                                                                                                 >  2:829896/1‑129 (MQ=255)
                                                     ttCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAgg                                                                                         >  1:1325512/1‑151 (MQ=255)
                                                       cACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTGGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAgg                                                                                         <  2:835956/149‑1 (MQ=255)
                                                         ccGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAg                                                                                      <  2:1429373/150‑1 (MQ=255)
                                                         ccGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACAAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTa                                                                                       <  4:474725/149‑1 (MQ=255)
                                                          cgcgCAGTTCAACCAGGCCCTCTTCCAGACGTTTGCGAAAAATCGGATCGTCAGAGAGTTGGGAGTTGAACAGCATAGTGACCAGGGAGGACACCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCt                                                                                    <  1:1322534/151‑1 (MQ=255)
                                                               aGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGc                                                                               >  2:1204993/1‑151 (MQ=255)
                                                               aGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGc                                                                               >  4:58068/1‑151 (MQ=255)
                                                                gTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGcc                                                                              >  3:70184/1‑151 (MQ=255)
                                                                     aCCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGa                                                                          <  3:254960/150‑1 (MQ=255)
                                                                      ccAGGCCCTCTCCCAGACGCTTGCGATAAATCGGACCGTCAGAGAGTTGGGAGTTGACCAGCATAGTGACCAGGGAGGCCATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGa                                                                          <  2:1525698/149‑1 (MQ=255)
                                                                       cAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCt                                                                       >  2:1325781/1‑151 (MQ=255)
                                                                        aGGCCCTCTTCCAGACGCTTGCGCTAAATCGGATCGTCCGAGAGTTTGGAGTTGAACAGCATAGAGACTAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGAAGGGATGACCACGGAGAGCAGATGGAGGAAGCTGATGCCATGACCtt                                                                      >  1:86135/1‑151 (MQ=255)
                                                                         ggCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTc                                                                     >  2:265443/1‑151 (MQ=255)
                                                                           cccTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTcc                                                                    >  2:548457/1‑150 (MQ=255)
                                                                            ccTCTTCCAGACGCTGGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAAAGCAAAGTGACCAGGGAGAAAATCCCCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCAt                                                                  <  2:1259717/151‑1 (MQ=255)
                                                                              tctTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTGGAACAGCATAGTGACAGGGGAGGCAACCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCAt                                                                  <  4:30781/149‑1 (MQ=255)
                                                                                 tCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACgg                                                             >  1:725508/1‑151 (MQ=255)
                                                                                  ccggACGCTGGCGAAAAATCGGACTGTCAGAGAGTTTGGGGTGGAAAAGGGAAGTGACCGGGGAGGAAATCCCGAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACgg                                                             <  2:335477/147‑1 (MQ=255)
                                                                                  ccAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACg                                                              <  1:247761/149‑1 (MQ=255)
                                                                                  ccAGACGCTTGCAATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCCCCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGa                                                            <  3:365474/151‑1 (MQ=255)
                                                                                         cTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATg                                                     >  1:1734977/1‑151 (MQ=255)
                                                                                          ttGCTATAAACCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACACCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGAc                                                        <  2:455122/147‑1 (MQ=255)
                                                                                          ttGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGt                                                    >  2:1602080/1‑151 (MQ=255)
                                                                                             cGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTaaa                                                 <  2:1534820/151‑1 (MQ=255)
                                                                                                 aaaTCGGATCGTCAGAGAGTTTGGAGTTGAACAGAATAGTGACCAGGGAGGAAATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCAc                                             <  4:435603/151‑1 (MQ=255)
                                                                                                 aaaCCGGATCGTCAGAGAGTTTGGAGTTGAAAACCATGGTAACAAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCa                                              <  1:348371/150‑1 (MQ=255)
                                                                                                   aTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTg                                           >  2:1324965/1‑151 (MQ=255)
                                                                                                   aTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTg                                           >  2:434056/1‑151 (MQ=255)
                                                                                                   aTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGCGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTg                                           >  2:488194/1‑151 (MQ=255)
                                                                                                    tCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGc                                          >  1:86290/1‑151 (MQ=255)
                                                                                                      ggATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCg                                        >  1:729973/1‑151 (MQ=255)
                                                                                                      ggATCGTCAGAGAGTTGGGAGTGGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGc                                          <  4:113381/149‑1 (MQ=255)
                                                                                                        aTCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCt                                      >  2:1725829/1‑151 (MQ=255)
                                                                                                        aTCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCt                                      >  3:22331/1‑151 (MQ=255)
                                                                                                         tCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTg                                     >  2:940296/1‑151 (MQ=255)
                                                                                                           gTCAGAGAGTTTGGAGTTGAACAGCATAGTGACTGGGCAGGACATCACCAG‑AACCGCCAGCAGGGGGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTg                                     >  1:214155/1‑149 (MQ=255)
                                                                                                           gTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTg                                     >  3:502059/1‑149 (MQ=255)
                                                                                                             cAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTg                                     >  2:1357398/1‑147 (MQ=255)
                                                                                                              agagagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGAtt                                >  1:1546228/1‑151 (MQ=255)
                                                                                                              agagagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGAtt                                >  3:317418/1‑151 (MQ=255)
                                                                                                              agagagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGAtt                                >  4:252851/1‑151 (MQ=255)
                                                                                                              agagagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGAtt                                >  2:668164/1‑151 (MQ=255)
                                                                                                               gagagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTg                               >  2:1056811/1‑151 (MQ=255)
                                                                                                               gagagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACACCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTgaga                                  <  2:1325658/148‑1 (MQ=255)
                                                                                                                 gagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGc                             >  2:457137/1‑151 (MQ=255)
                                                                                                                   gTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCg                            <  1:1612631/150‑1 (MQ=255)
                                                                                                                    tttGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGAt                          >  4:197341/1‑151 (MQ=255)
                                                                                                                      tGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGc                        >  1:912687/1‑151 (MQ=255)
                                                                                                                       ggAGTTAAACAGCATAGTGACCAGGGGGGACACCCAAAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGAt                          <  1:1002311/148‑1 (MQ=255)
                                                                                                                        gAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCgg                      >  1:1657205/1‑151 (MQ=255)
                                                                                                                           ttGAACAGCATAGTGCCCAGGGAGGACATCCCCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTg                    <  1:1719051/150‑1 (MQ=255)
                                                                                                                           ttGAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTg                    <  1:1459094/150‑1 (MQ=255)
                                                                                                                             gAACAGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATc                 >  3:511268/1‑151 (MQ=255)
                                                                                                                               acaacaTAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACAGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGc               <  2:1212365/151‑1 (MQ=255)
                                                                                                                                 aGCATAGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCa              <  2:1047658/150‑1 (MQ=255)
                                                                                                                                      aGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTgc        >  2:1494/1‑151 (MQ=255)
                                                                                                                                      aGTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTgc        >  3:123137/1‑151 (MQ=255)
                                                                                                                                      aGTGACCAGGGAGGACATCACAAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTg         <  4:25540/150‑1 (MQ=255)
                                                                                                                                       gTGACCAGGGAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTgc        <  2:725616/150‑1 (MQ=255)
                                                                                                                                        tGACCAGGGGGAACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATc           <  1:1346587/146‑1 (MQ=255)
                                                                                                                                        tGACCAGGGAGGACACCCCCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTg         <  1:707736/148‑1 (MQ=255)
                                                                                                                                             agggaggaCATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAt  >  2:627635/1‑150 (MQ=255)
                                                                                                                                             agggaggaCATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAt  >  1:555189/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAG‑AACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAT  >  NC_000913/4366079‑4366369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A