Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,366,223	A→G	F184S (TTC→TCC)	dcuA ←	C4‑dicarboxylate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,223	0	A	G	97.0%	102.2 / ‑4.0	33	F184S (TTC→TCC)	dcuA	C4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (17/15); total (18/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

AGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAT  >  NC_000913/4366079‑4366369

agcagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACa                                                                                                                                              <  2:99992/151‑1 (MQ=255)

  cagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATc                                                                                                                                            <  2:15089/151‑1 (MQ=255)

  cagGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATc                                                                                                                                            <  1:577090/151‑1 (MQ=255)

    ggAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCAc                                                                                                                                          <  1:336171/151‑1 (MQ=255)

            cAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCg                                                                                                                                  >  1:563258/1‑151 (MQ=255)

                 ggacgTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCcagc                                                                                                                             >  1:428039/1‑151 (MQ=255)

                                      gatttCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACg                                                                                                        >  2:529119/1‑151 (MQ=255)

                                          tCAATCTGTTTTTCCCCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGgag                                                                                                     <  1:576487/150‑1 (MQ=255)

                                            aaTCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  2:290784/1‑151 (MQ=255)

                                               cTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGc                                                                                                  >  1:227026/1‑148 (MQ=255)

                                                  cTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATgg                                                                                            >  2:606585/1‑151 (MQ=255)

                                                   ttttCCCCGCGCAGTTCAACCGGGCCCTCTTCCAGACGCTTGCAATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGAATAGTGACCAGGGAGGACATCCCAAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATgg                                                                                            <  2:614656/150‑1 (MQ=255)

                                                   ttttCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAAGGGGATCGTCAGAGAGTTGGGAGTTGAACAGCATAGTGACCAGGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGa                                                                                               <  1:397061/147‑1 (MQ=255)

                                                    tttCACCGCGAAGTTCAACCAGCCCCTCTTCCAGACGCTTGCGATAAAGCGGATCGTCAGAGAGTTGGGAGTTGAACAGCATAGTGCCCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATgg                                                                                            <  1:327077/149‑1 (MQ=255)

                                                      tCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAgg                                                                                         >  2:616508/1‑150 (MQ=255)

                                                          cgcgCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCt                                                                                    >  1:167205/1‑151 (MQ=255)

                                                               aGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGc                                                                               >  2:254389/1‑151 (MQ=255)

                                                                        cggCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCtt                                                                      >  2:378809/2‑151 (MQ=255)

                                                                              tctTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATc                                                                 >  1:226270/1‑150 (MQ=255)

                                                                                   cAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGaa                                                           <  1:463976/151‑1 (MQ=255)

                                                                                     gACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGa                                                            >  1:526164/1‑148 (MQ=255)

                                                                                            gCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTa                                                   >  1:129065/1‑150 (MQ=255)

                                                                                                  aaTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACt                                            >  1:516196/1‑151 (MQ=255)

                                                                                                  aaTCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACt                                            <  2:307327/151‑1 (MQ=255)

                                                                                                     cGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGcc                                         >  1:488341/1‑151 (MQ=255)

                                                                                                     cGGAGCGTGAGAGAGTTTGGAGTTAAACAGCATAGTAACCAGGGAGGACATCACCAAAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTg                                           <  1:588870/149‑1 (MQ=255)

                                                                                                          cGTCAGAGATTTGGGAGTTAAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTg                                     <  2:560736/150‑1 (MQ=255)

                                                                                                              agagagTTTGGAGTTGAACAGCATAGTGACCAGGGAGGACATCCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGAt                                 <  1:308852/150‑1 (MQ=255)

                                                                                                                        gAGTTGAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCgg                      >  2:206449/1‑151 (MQ=255)

                                                                                                                             gAACAGCATAGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGAt                  <  1:434767/150‑1 (MQ=255)

                                                                                                                                      aGTGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCt          <  1:599533/149‑1 (MQ=255)

                                                                                                                                        tGACCAGGGAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTgcgc      >  1:250250/1‑151 (MQ=255)

                                                                                                                                           ccAGGAAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCgg    >  1:166243/1‑150 (MQ=255)

                                                                                                                                             agggaggaCATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAt  >  2:53395/1‑150 (MQ=255)

                                                                                                                                             agggaggaCATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAt  <  1:187617/150‑1 (MQ=255)

                                                                                                                                             agggaggaCATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAt  >  1:460960/1‑150 (MQ=255)

AGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCTGCTTTTCACCGCGCAGTTCAACCAGGCCCTCTTCCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAACAGCATAGTGACCAGGAAGGACATCACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAGACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAT  >  NC_000913/4366079‑4366369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A