Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,856,193	G→A	L39F (CTT→TTT)	yidH ←	DUF202 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,856,193	0	G	A	100.0%	134.9 / NA	37	L39F (CTT→TTT)	yidH	DUF202 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/17); total (20/17)

TCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTAC  >  NC_000913/3856058‑3856340

tCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCcgccgc                                                                                                                                      >  2:238539/1‑151 (MQ=255)

      gCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGaa                                                                                                                                 >  2:211813/1‑150 (MQ=255)

      gCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGaa                                                                                                                                 >  1:72931/1‑150 (MQ=255)

       cATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGaa                                                                                                                                 <  2:421725/149‑1 (MQ=255)

       cATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAAt                                                                                                                               >  2:144656/1‑151 (MQ=255)

        aTCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATc                                                                                                                              <  1:687625/151‑1 (MQ=255)

           gCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCt                                                                                                                            >  1:277174/1‑150 (MQ=255)

                  cATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAg                                                                                                                     <  1:248235/150‑1 (MQ=255)

                           aCCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATcc                                                                                                            >  2:685524/1‑150 (MQ=255)

                             cAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACg                                                                                                         >  2:555647/1‑151 (MQ=255)

                             cAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACg                                                                                                         >  2:477645/1‑151 (MQ=255)

                                      aaGCCATACATCGCAAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGaaaa                                                                                                 <  2:580232/149‑1 (MQ=255)

                                        gCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGt                                                                                               >  2:275669/1‑150 (MQ=255)

                                          cATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACg                                                                                            >  2:344762/1‑151 (MQ=255)

                                           aTACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCAAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGc                                                                                           >  2:484809/1‑151 (MQ=255)

                                             aCATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTc                                                                                         >  2:759992/1‑151 (MQ=255)

                                               aTCGCTACCCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCAt                                                                                       <  2:400567/151‑1 (MQ=255)

                                                            cgGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGaa                                                                          <  2:590934/151‑1 (MQ=255)

                                                                  aCAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGt                                                                     >  1:710354/1‑150 (MQ=255)

                                                                       cacaACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAg                                                               <  1:290906/151‑1 (MQ=255)

                                                                            caacaacGCCAGGAGTTCGCGAATGACGGGCGTGGGGAAAGCTGGCGCAAGTTGGTCAAACCCAACCCCCGACGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGGACGCTGATTAGCCAGCGAGAAGCGGTAATCAGgcgc                                                           <  2:94697/150‑1 (MQ=255)

                                                                            caacaacGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCt                                                          >  1:415921/1‑151 (MQ=255)

                                                                                  cGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCg                                                    <  1:760037/151‑1 (MQ=255)

                                                                                      agcagTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGc                                                >  2:235119/1‑151 (MQ=255)

                                                                                           ttCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCggg                                             >  2:109168/1‑149 (MQ=255)

                                                                                                  aTGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCa                                    <  1:685566/151‑1 (MQ=255)

                                                                                                          cGTGGCGAAATCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCt                            <  2:245912/151‑1 (MQ=255)

                                                                                                            tGGCGCAATCTGGCCCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGGACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAATCGGTAATCAGGCGCTTCTCCGAGGCGGGGAATGTTCAatgatccta                           >  2:38503/1‑144 (MQ=255)

                                                                                                                  aaTCTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGc                     >  1:604360/1‑150 (MQ=255)

                                                                                                                     cTGGCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTTGATGTTTCTCCtttttgatt                 >  2:455805/1‑145 (MQ=255)

                                                                                                                       ggCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAAt                  <  1:628141/148‑1 (MQ=255)

                                                                                                                       ggCGCAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTc               <  2:242428/151‑1 (MQ=255)

                                                                                                                        gcgcAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCa              <  1:723628/151‑1 (MQ=255)

                                                                                                                           cAAGTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCCTTGTTGCTCCTTTGCAATTTCACt            >  2:758724/1‑150 (MQ=255)

                                                                                                                              gTTGATCAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACg        <  1:82870/151‑1 (MQ=255)

                                                                                                                               tttaTCAAACCCAGCCGACGGCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTAATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTa           <  2:566387/144‑1 (MQ=255)

                                                                                                                                    cAAACCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTAc  <  1:331727/151‑1 (MQ=255)

TCAGACGCATCGCTTTTTCATTGCGTAACCAGCGCAAGTAGCCATACATCGCTAACCCGCCGGAAAACAGACACAACAACAACGCCAGCAGTTCGCGAATGACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTAC  >  NC_000913/3856058‑3856340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A