Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,929,378 A→G C76R (TGC→CGC)  viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,929,3780AG100.0% 215.8 / NA 59C76R (TGC→CGC) viaAstimulator of RavA ATPase activity; von Willebrand factor domain protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/30);  total (29/30)

TAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCGGTGAGGCC  >  NC_000913/3929232‑3929523
                                                                                                                                                  |                                                                                                                                                 
tAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATc                                                                                                                                               >  2:605873/1‑151 (MQ=255)
  aCGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCAc                                                                                                                                             >  1:719458/1‑151 (MQ=255)
       gAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACctc                                                                                                                                          <  2:752555/149‑1 (MQ=255)
         aGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCg                                                                                                                                      >  1:355395/1‑151 (MQ=255)
             aTCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTg                                                                                                                                   >  2:726957/1‑150 (MQ=255)
             aTCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGa                                                                                                                                  <  2:743928/151‑1 (MQ=255)
              tCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTg                                                                                                                                   >  1:257474/1‑149 (MQ=255)
               cGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCAACACCTCTTCGGTGAGt                                                                                                                                >  1:541847/1‑151 (MQ=255)
                gTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGtt                                                                                                                               <  1:111105/151‑1 (MQ=255)
                gTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGtt                                                                                                                               <  2:285554/151‑1 (MQ=255)
                  gCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTc                                                                                                                              <  1:664022/150‑1 (MQ=255)
                   cTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGGGAGTTCcg                                                                                                                            >  2:667188/1‑151 (MQ=255)
                     gTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCcg                                                                                                                            <  1:241389/149‑1 (MQ=255)
                       tAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCcggcg                                                                                                                         <  1:326052/150‑1 (MQ=255)
                        aGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCggg                                                                                                                       >  2:385801/1‑151 (MQ=255)
                          cATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGAc                                                                                                                     <  1:456911/151‑1 (MQ=255)
                          cATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGAc                                                                                                                     <  1:182251/151‑1 (MQ=255)
                           aTCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGAc                                                                                                                     >  2:138341/1‑150 (MQ=255)
                             caaccaacTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGGTGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTc                                                                                                                   <  1:630184/150‑1 (MQ=255)
                             caaccaacTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCg                                                                                                                  <  1:148969/151‑1 (MQ=255)
                                      gTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCttt                                                                                                         <  1:273881/151‑1 (MQ=255)
                                      gTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCtt                                                                                                          <  2:230314/150‑1 (MQ=255)
                                        cGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTGCa                                                                                                       <  2:683343/151‑1 (MQ=255)
                                           gCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGcc                                                                                                    <  1:477812/151‑1 (MQ=255)
                                                 tCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGAc                                                                                                 >  2:382790/1‑148 (MQ=255)
                                                 tCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTgc                                                                                              >  1:104047/1‑151 (MQ=255)
                                                    gCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTgcgc                                                                                            <  1:418664/150‑1 (MQ=255)
                                                      ccATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAg                                                                                          >  1:101558/1‑150 (MQ=255)
                                                            agaATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCa                                                                                   <  1:144685/151‑1 (MQ=255)
                                                                 tCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGcc                                                                               >  1:244391/1‑150 (MQ=255)
                                                                 tCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGcc                                                                               >  1:180158/1‑150 (MQ=255)
                                                                  cAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCa                                                                              <  2:754786/150‑1 (MQ=255)
                                                                          gCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGaa                                                                      <  2:302546/150‑1 (MQ=255)
                                                                               aaGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAcatca                                                                 >  1:587005/1‑150 (MQ=255)
                                                                                aGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACatcatc                                                               >  1:638991/1‑151 (MQ=255)
                                                                                   ccAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGt                                                            <  2:725584/151‑1 (MQ=255)
                                                                                      ggATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGAt                                                         <  2:351721/151‑1 (MQ=255)
                                                                                         tCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGAtt                                                        >  2:760258/1‑149 (MQ=255)
                                                                                         tCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTgc                                                      >  2:194186/1‑151 (MQ=255)
                                                                                            gtgGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGAtt                                                        <  1:754582/146‑1 (MQ=255)
                                                                                              ggTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCt                                                 <  1:119240/151‑1 (MQ=255)
                                                                                               gTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCtt                                                >  1:46889/1‑151 (MQ=255)
                                                                                                tAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAAAATCATCAATGATTGTCGCCtt                                                >  2:418725/1‑150 (MQ=255)
                                                                                                     gCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCCTc                                               >  2:520074/1‑146 (MQ=255)
                                                                                                         aaTAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTggg                                      <  1:15295/151‑1 (MQ=255)
                                                                                                            aaaCTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGaa                                    <  2:265043/150‑1 (MQ=255)
                                                                                                            aaaCTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGaa                                    <  2:324471/150‑1 (MQ=255)
                                                                                                             aaCTGTGGCGTCGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAAtt                                  >  1:3049/1‑151 (MQ=255)
                                                                                                              aCTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAAttt                                 <  1:326197/151‑1 (MQ=255)
                                                                                                                tgtgGCGTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGGATCTTTCAGCCGACTCCGCAGCGCCTCACGCCCGCGGGGGAAATCAACAGCGATTTGCCCCTTCCGGCGTGGGAAttttt                               >  2:78714/1‑151 (MQ=255)
                                                                                                                      gTGGAGAGGAGCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAaaga                         <  1:278587/151‑1 (MQ=255)
                                                                                                                                gCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGcc                 >  1:9559/1‑149 (MQ=255)
                                                                                                                                gCTGGCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCa                <  2:290724/150‑1 (MQ=255)
                                                                                                                                   ggCTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCCGCGCTTCACGCCAGCGGGGAACCTCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCt             >  2:391889/1‑150 (MQ=255)
                                                                                                                                     cTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCg          >  1:394221/1‑151 (MQ=255)
                                                                                                                                     cTTTGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCg          >  1:535160/1‑151 (MQ=255)
                                                                                                                                      tttGCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCgg         >  2:393655/1‑151 (MQ=255)
                                                                                                                                         gCTGATAGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCGGTGa      <  2:324374/151‑1 (MQ=255)
                                                                                                                                               aGCGCATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCGGTGAGGcc  >  2:53718/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TAACGCTGAAGTGATCGTGCTGTTAGCATCAACCAACTGTCGGGCTTGTTCTGCCCATGGAGAATTCAGACGATGCAGTAAGTCCAGGATCTGTGGTAGCTGCACAATAAACTGTGGCGTGGAGAGGAGCTGGCTTTGCTGATAGCACATCACCTCTTCGGTGAGTTCCGGCGGGACTCGGGCATCTTTCAGCCGACTGCGCAGCGCTTCACGCCAGCGGGGAACATCATCAGTGATTGCCGCCTTCAGGCGTGGGAATTTTTCAAAGAAGACCGCCAGCTGCGGTGAGGCC  >  NC_000913/3929232‑3929523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A