Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F50 I2 R1
|
209 |
44.2 |
1360995 |
98.7% |
1343302 |
150.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,505,884 |
A→G |
L46P (CTG→CCG) |
yhfS ← |
FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,505,884 | 0 | A | G | 100.0%
| 83.7
/ NA
| 27 | L46P (CTG→CCG) | yhfS | FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/17); total (10/17) |
CCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGC > NC_000913/3505738‑3506026
|
ccAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑c < 1:449013/150‑1 (MQ=255)
cAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCt < 2:576421/151‑1 (MQ=255)
cGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTa < 1:129888/151‑1 (MQ=255)
gCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAg < 1:141384/149‑1 (MQ=255)
ccccGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCt < 2:451249/151‑1 (MQ=255)
tttGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑Tc > 2:138223/1‑150 (MQ=255)
aaaGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGcc > 1:14075/1‑149 (MQ=255)
ccgccAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCTTTAAGCCTAAA‑TCACCGCCAGTAAg < 1:159427/151‑1 (MQ=255)
cgccAGCCCTGCGCGAATCGCGCCAATCCACGCGCCCTGCACCAGCGCCTCAGCCTGTGCGTGAAGTGCATCAGCCAGCACCTGTTCCACACGCTGGGGAACGCGCGTTTGATTCGGT‑CCTGTCGTTAAGCCTAAA‑TCACCGCCGGTa > 2:502878/1‑148 (MQ=255)
cccGGCGCGAATCGCGCCATCCCCCGCCCCCTGCCCCACCCCCGCAGCCTGTGCGTAAAATCCATCACCCAGCCCTTTTCCCACACGCTGGGTACCGCGCGGTTGATTCGTT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACt < 2:425344/149‑1 (MQ=255)
cgcCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCCCCTGTCCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCt < 1:193932/151‑1 (MQ=255)
ccgtGCCCCGGCGCCGCATCCTGGGTCGGAAATGCATCGGCCAGCACCGCTCCCACCCGCGGGGAAAGGCGCGG‑TGGTTGGGTCCCTGGGGTTAGGCCTAAA‑TCGCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTAt < 2:482382/148‑1 (MQ=255)
ccgcGGCCGGTGCGTGAAATGCATCAGCCAGCACCTGTCCCACCCGCTGGGTACCGCGCGGTTGTTTCGGT‑CCGGGCGTAAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAgc < 2:107033/146‑1 (MQ=255)
cgcgGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCAAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAAc < 2:365909/151‑1 (MQ=255)
gtgCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtg > 1:537760/1‑151 (MQ=255)
gtaaaaGGCATCAGCCAGCCCCTGTCCCACCCGCGGGGTACCGCGCGGTTGATTCGGT‑CCTGGCTTAAACCCAAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgctg < 2:536741/147‑1 (MQ=255)
gTGAAATGCATCAGCCAGCCCGTGTCCCCCACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTtgctgc < 1:335953/151‑1 (MQ=255)
tGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTc > 2:229759/1‑150 (MQ=255)
tCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCaata > 1:326302/1‑150 (MQ=255)
tCCACACGCTGGGTAACGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGc > 2:432813/1‑150 (MQ=255)
gCTGGGTAACGCGCGGTTGTTTCGGT‑CCTGGCGTTAAGCCTAAA‑CCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGa < 1:140888/149‑1 (MQ=255)
tGGGTACCGCGCGTTTGTTTCGGT‑CTTGGGGTTAAGCTTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAAc < 1:188161/150‑1 (MQ=255)
tGGGAAACGCGCGTTTGATCCGGT‑CCTGGCTTTAA‑CCTAAACCCGCCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCagag < 1:170971/145‑1 (MQ=255)
gggTAACGCGCGGTTGATTCGGT‑CCGGGCGTTAAGCCAAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAAc < 1:536369/149‑1 (MQ=255)
aCGCGCGGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTc > 2:143592/1‑150 (MQ=255)
cgcgGTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCAt > 2:520966/1‑149 (MQ=255)
gTTGATTCGGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGc > 1:104689/1‑151 (MQ=255)
|
CCAGAAGACGCTGCCCCGGTTTGAGCAAAGCCGCCAGCCCGGCGCGAATCGCGCCAGTCCCCGCGCCCTGCACCAGCGCCGCAGCCTGTGCGTGAAATGCATCAGCCAGCACCTGTTCCACACGCTGGGTAACGCGCGGTTGATTCAGT‑CCTGGCGTTAAGCCTAAA‑TCACCGCCAGTAAGAAACTCGCTGCCGGGGAAATGGCGACAAATGCTATCCACCAGCGCAAACTGCTTTTGCTGCGCCTCAATAATCGTCAGGCTTTGCAGAGGAAACGTCTTCATAGGTGC > NC_000913/3505738‑3506026
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
