Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,134,394 A→G D187G (GAC→GGC)  katG → catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,134,3940AG100.0% 166.9 / NA 45D187G (GAC→GGC) katGcatalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/22);  total (23/22)

GCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCG  >  NC_000913/4134247‑4134540
                                                                                                                                                   |                                                                                                                                                  
gCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGt                                                                                                                                                 >  2:39686/1‑151 (MQ=255)
        aaaGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAAc                                                                                                                                         >  2:611933/1‑151 (MQ=255)
          aGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCg                                                                                                                                       <  1:283927/151‑1 (MQ=255)
             gcgTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGa                                                                                                                                     >  2:217987/1‑150 (MQ=255)
                          tgGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACt                                                                                                                       >  2:29658/1‑151 (MQ=255)
                           ggCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTg                                                                                                                      <  2:242631/151‑1 (MQ=255)
                              cAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTgggg                                                                                                                   >  1:175023/1‑151 (MQ=255)
                                       aGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGaaaa                                                                                                          <  1:459314/151‑1 (MQ=255)
                                        gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGaaaa                                                                                                          <  2:203498/150‑1 (MQ=255)
                                             atGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCt                                                                                                     <  1:562787/150‑1 (MQ=255)
                                             atGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTg                                                                                                    <  1:551302/151‑1 (MQ=255)
                                               ggTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGc                                                                                                  >  1:532359/1‑151 (MQ=255)
                                                       aaTCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCAc                                                                                          <  1:203779/151‑1 (MQ=255)
                                                         tctcCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCg                                                                                        >  2:110465/1‑151 (MQ=255)
                                                            ccTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGt                                                                                       <  2:194568/149‑1 (MQ=255)
                                                            ccTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCa                                                                                     <  1:601706/151‑1 (MQ=255)
                                                                   cGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGCTCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGa                                                                               >  2:353757/1‑150 (MQ=255)
                                                                       cTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAgcgc                                                                          >  2:170529/1‑151 (MQ=255)
                                                                            tATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGc                                                                      <  2:439088/150‑1 (MQ=255)
                                                                               ccTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGaa                                                                   >  1:592716/1‑150 (MQ=255)
                                                                                      ggTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGc                                                           >  1:5942/1‑151 (MQ=255)
                                                                                         aaCGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTgg                                                        <  2:244673/151‑1 (MQ=255)
                                                                                           cGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGt                                                      >  2:96497/1‑151 (MQ=255)
                                                                                            gTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTg                                                     >  1:142266/1‑151 (MQ=255)
                                                                                            gTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTg                                                     >  1:507464/1‑151 (MQ=255)
                                                                                                cgcTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAAc                                                 <  1:24714/151‑1 (MQ=255)
                                                                                                    aGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCgag                                             <  2:244162/151‑1 (MQ=255)
                                                                                                               gCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGAt                                  >  2:645922/1‑151 (MQ=255)
                                                                                                               gCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGAt                                  <  2:333093/151‑1 (MQ=255)
                                                                                                                   ccGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTa                               <  1:105581/150‑1 (MQ=255)
                                                                                                                     gTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGt                            >  2:417133/1‑151 (MQ=255)
                                                                                                                       aCCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGtt                           >  2:288582/1‑150 (MQ=255)
                                                                                                                           tCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAAccc                      >  2:626880/1‑151 (MQ=255)
                                                                                                                           tCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAAcc                       <  1:446392/150‑1 (MQ=255)
                                                                                                                             ggTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCgg                    >  1:331393/1‑151 (MQ=255)
                                                                                                                             ggTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCgg                    <  2:428009/151‑1 (MQ=255)
                                                                                                                              gTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGa                   <  2:156676/151‑1 (MQ=255)
                                                                                                                               ttttGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACCGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGa                   >  2:177120/1‑150 (MQ=255)
                                                                                                                                tttGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGa                   >  2:667262/1‑149 (MQ=255)
                                                                                                                                   ggTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGcc              >  1:251620/1‑151 (MQ=255)
                                                                                                                                   ggTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGc               <  2:493154/150‑1 (MQ=255)
                                                                                                                                   ggTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGc               <  1:205141/150‑1 (MQ=255)
                                                                                                                                        cGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGAt         >  1:431631/1‑151 (MQ=255)
                                                                                                                                             gTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAg    <  2:276433/151‑1 (MQ=255)
                                                                                                                                               gAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcg  <  2:430227/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCTCGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCG  >  NC_000913/4134247‑4134540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A