Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,134,394	A→G	D187G (GAC→GGC)	katG →	catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,134,394	0	A	G	100.0%	172.7 / NA	46	D187G (GAC→GGC)	katG	catalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/25); total (21/25)

CGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGG  >  NC_000913/4134251‑4134541

cGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCt                                                                                                                                                <  2:580261/149‑1 (MQ=255)

cGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTgg                                                                                                                                              <  2:485784/151‑1 (MQ=255)

 gATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTggg                                                                                                                                             >  2:239484/1‑151 (MQ=255)

    aaaGCGCGTCGACTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCACGGGTAACGAGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAAc                                                                                                                                          >  1:537659/1‑151 (MQ=255)

            tCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCt                                                                                                                                                <  1:106274/137‑1 (MQ=255)

            tCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCt                                                                                                                                                >  2:106275/1‑137 (MQ=255)

               ccTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGAt                                                                                                                               <  1:534547/151‑1 (MQ=255)

                       ggCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTg                                                                                                                       <  2:256352/151‑1 (MQ=255)

                        gCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTgg                                                                                                                      <  2:378680/151‑1 (MQ=255)

                              cAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGtga                                                                                                                 <  1:506268/150‑1 (MQ=255)

                               aaaCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGtgatg                                                                                                               >  2:487198/1‑151 (MQ=255)

                                aaCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGtgatg                                                                                                               >  2:667587/1‑150 (MQ=255)

                                 aCAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGaa                                                                                                             <  1:588180/151‑1 (MQ=255)

                                    gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGaaaaa                                                                                                          <  1:461794/151‑1 (MQ=255)

                                    gAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGaaaaa                                                                                                          <  2:159491/151‑1 (MQ=255)

                                     aaaTATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAg                                                                                                         <  2:682481/151‑1 (MQ=255)

                                         atGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCt                                                                                                      <  2:41630/150‑1 (MQ=255)

                                                       tcCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTc                                                                                       >  1:463825/1‑151 (MQ=255)

                                                        ccTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTc                                                                                       >  2:401250/1‑150 (MQ=255)

                                                        ccTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCa                                                                                      <  2:494533/151‑1 (MQ=255)

                                                          tGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATc                                                                                    >  1:193083/1‑151 (MQ=255)

                                                            ggCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCg                                                                                  <  1:576153/151‑1 (MQ=255)

                                                            ggCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCg                                                                                  <  1:128489/151‑1 (MQ=255)

                                                                 aCCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAgc                                                                             <  2:234376/151‑1 (MQ=255)

                                                                             tCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGc                                                                 >  2:545596/1‑151 (MQ=255)

                                                                              cgcgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGc                                                                 >  2:203745/1‑150 (MQ=255)

                                                                              cgcgGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCa                                                                <  1:33799/151‑1 (MQ=255)

                                                                                       cGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTggg                                                        >  2:358311/1‑150 (MQ=255)

                                                                                                   aaaCTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGGGATg                                           <  1:25723/151‑1 (MQ=255)

                                                                                                      cTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGCGATGGGt                                        >  1:571368/1‑151 (MQ=255)

                                                                                                       tCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTc                                       >  2:215932/1‑151 (MQ=255)

                                                                                                        ccGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTc                                       <  1:203445/150‑1 (MQ=255)

                                                                                                          ggCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTg                                     >  2:178810/1‑150 (MQ=255)

                                                                                                              tCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGAttt                                 >  2:72677/1‑150 (MQ=255)

                                                                                                                   aCCTTCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTa                           <  1:603874/151‑1 (MQ=255)

                                                                                                                      ttCGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAAcc                        >  2:633149/1‑151 (MQ=255)

                                                                                                                        cGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                      <  2:401433/151‑1 (MQ=255)

                                                                                                                        cGGTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCg                      <  2:438435/151‑1 (MQ=255)

                                                                                                                         ggTTTTGGTGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCgg                     >  2:477926/1‑151 (MQ=255)

                                                                                                                                 tGCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCg             >  1:575808/1‑151 (MQ=255)

                                                                                                                                  gCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCgg            >  1:374465/1‑151 (MQ=255)

                                                                                                                                  gCCGGTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCg             <  2:249521/150‑1 (MQ=255)

                                                                                                                                     ggTCGTGAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATc         >  1:102022/1‑151 (MQ=255)

                                                                                                                                           gAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcg   >  1:579330/1‑151 (MQ=255)

                                                                                                                                           gAAGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcg   <  2:101999/151‑1 (MQ=255)

                                                                                                                                            aaGGCGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAgcgg  <  2:620847/151‑1 (MQ=255)

CGATAAAGCGCGTCGCCTGTTGTGGCCAATCAAACAGAAATATGGTCAGAAAATCTCCTGGGCCGACCTGTTTATCCTCGCGGGTAACGTGGCGCTAGAAAACTCCGGCTTCCGTACCTTCGGTTTTGGTGCCGGTCGTGAAGACGTCTGGGAACCGGATCTGGATGTTAACTGGGGTGATGAAAAAGCCTGGCTGACTCACCGTCATCCGGAAGCGCTGGCGAAAGCACCGCTGGGTGCAACCGAGATGGGTCTGATTTACGTTAACCCGGAAGGCCCGGATCACAGCGG  >  NC_000913/4134251‑4134541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A