Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,003,303	A→G	R292R (CGT→CGC)	rarD ←	putative chloramphenical resistance permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,003,303	0	A	G	100.0%	122.0 / NA	33	R292R (CGT→CGC)	rarD	putative chloramphenical resistance permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/17); total (16/17)

TACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTAC  >  NC_000913/4003160‑4003441

tACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCt                                                                                                                                        <  1:58627/148‑1 (MQ=255)

      tAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATaa                                                                                                                                <  1:280044/150‑1 (MQ=255)

            ttGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcat                                                                                                                         >  2:30396/1‑151 (MQ=255)

             tGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatc                                                                                                                        >  1:464698/1‑151 (MQ=255)

              gggATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatc                                                                                                                        <  2:146702/150‑1 (MQ=255)

                gATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGcatccat                                                                                                                     <  2:526762/151‑1 (MQ=255)

                          cGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAAtt                                                                                                           <  1:104059/151‑1 (MQ=255)

                           gTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTg                                                                                                          >  1:669603/1‑151 (MQ=255)

                              aCATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCa                                                                                                       <  2:590040/151‑1 (MQ=255)

                                                tGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAg                                                                                      <  1:59012/150‑1 (MQ=255)

                                                     gAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGaaa                                                                                <  1:98119/151‑1 (MQ=255)

                                                     gAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGaa                                                                                 >  2:628999/1‑150 (MQ=255)

                                                      aGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATTCATCACAAAAATTGCCAGCGACACCCAAATAAAGGCGaa                                                                                 >  1:547643/1‑149 (MQ=255)

                                                             ggTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCAt                                                                        >  2:47818/1‑151 (MQ=255)

                                                                  gCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTAt                                                                   <  2:443809/151‑1 (MQ=255)

                                                                    aTGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCg                                                                 >  2:621776/1‑151 (MQ=255)

                                                                          atatGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCAccc                                                           >  2:200671/1‑151 (MQ=255)

                                                                                    tctcATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCAc                                                 >  2:160153/1‑151 (MQ=255)

                                                                                          gCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATaaa                                           <  2:63689/151‑1 (MQ=255)

                                                                                             agagTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATaaaa                                          >  1:558936/1‑149 (MQ=255)

                                                                                               agTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTc                                      >  1:13272/1‑151 (MQ=255)

                                                                                                    gCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagc                                 >  1:1240/1‑151 (MQ=255)

                                                                                                      atatTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagcc                                >  1:275893/1‑150 (MQ=255)

                                                                                                        atTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccagc                             >  2:239029/1‑151 (MQ=255)

                                                                                                        atTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCAcagccag                              <  1:119096/150‑1 (MQ=255)

                                                                                                          tttttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCcagca                            <  1:48099/150‑1 (MQ=255)

                                                                                                             ttCAGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGa                         >  2:502187/1‑150 (MQ=255)

                                                                                                                aGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACAt                     >  2:408301/1‑151 (MQ=255)

                                                                                                                aGAATATATTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACAt                     <  1:408296/151‑1 (MQ=255)

                                                                                                                     atatTTATTTTTTATTTGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGc                 <  2:660990/150‑1 (MQ=255)

                                                                                                                              ttttatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCa        <  1:292587/150‑1 (MQ=255)

                                                                                                                                 tatttGGACGTTCTGCGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATg     <  1:239028/150‑1 (MQ=255)

                                                                                                                                    ttGGACGTTCTGCGCTGCGTATGAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTAc  <  2:285535/150‑1 (MQ=255)

TACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGCATATTTTTTCAGAATATATTTATTTTTTATTTGGACGTTCTACGCTGCGTATAAATCGCATCCATCACAAAAATTGCCAGCGCCACCCAAATAAAGGCGAAAGTCACCATCTTATCGGCACCCGGTTTTTCACCATAAAACGTCACAGCCAGCAGGAACATCAGCGTCGGGCCAATGTAC  >  NC_000913/4003160‑4003441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A