Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F210 I1 R1	4928	385.2	6658600	97.5%	6492135	231.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	1,624,023	3 bp→TCA	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	1,624,023	0	A	T	99.8%	1232.3 / ‑5.8	508	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (256/251); minor base . (0/1); total (256/252)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,624,024	0	G	C	99.6%	1210.9 / NA	508	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (0/2); new base C (256/249); total (256/252)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,624,025	0	T	A	99.6%	1215.4 / NA	508	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (255/248); total (256/252)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 508 total aligned reads displayed.

CTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTAGTATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCAGTTGATTTAACGTCGGGGAAATGATCGCTCATGATTGAATTATTATTTCCCGGTTGGTTAGCCGGGATCATGCTCGCCTGTGCCGCGGGTCCGCTGGGTAGCTTTGTAGTCTGGCGTCGTATGTCTTATTTCGGTGATACGCTGGCTCATGCCAGTTTACTTGGCG  >  CP042583/1623743‑1624297

|||

    ggtggCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTc                                                                                                                                                                                                                                                                        <  1:1295620/289‑1 (MQ=255)

         cGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCg                                                                                                                                                                                                                                                                    <  2:1478337/288‑1 (MQ=255)

           aaaCGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCgtgg                                                                                                                                                                                                                                                                 >  2:2941762/1‑289 (MQ=255)

             aCGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCgtggtg                                                                                                                                                                                                                                                               <  1:1626630/289‑1 (MQ=255)

                           aTTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGt                                                                                                                                                                                                                                                 >  1:2432629/1‑289 (MQ=255)

                           aTTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGt                                                                                                                                                                                                                                                 >  2:1541107/1‑289 (MQ=255)

                             tAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGta                                                                                                                                                                                                                                                >  1:451648/1‑288 (MQ=255)

                               gcgcGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGt                                                                                                                                                                                                                                                                         <  1:1138045/261‑1 (MQ=255)

                               gcgcGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGtatct                                                                                                                                                                                                                                             >  1:509315/1‑289 (MQ=255)

                               gcgcGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCCGCATCCGGAGTTTATTTCAATGTTTGGTCCCCGTGGTGCTGAAAAAGTGGGtctct                                                                                                                                                                                                                                             >  2:1172878/1‑289 (MQ=255)

                                 gcgAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGtatcta                                                                                                                                                                                                                                            >  1:2468295/1‑288 (MQ=255)

                                    aGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTTGTGGTGCTGAACAACTGGGTATTTATCg                                                                                                                                                                                                                                         >  2:1358542/1‑288 (MQ=255)

                                    aGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCg                                                                                                                                                                                                                                         >  1:274495/1‑288 (MQ=255)

                                    aGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCg                                                                                                                                                                                                                                         >  1:3309593/1‑288 (MQ=255)

                                    aGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCg                                                                                                                                                                                                                                         >  1:2128858/1‑288 (MQ=255)

                                    aGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCg                                                                                                                                                                                                                                         >  1:1705177/1‑288 (MQ=255)

                                    aGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCg                                                                                                                                                                                                                                         >  2:1608503/1‑288 (MQ=255)

                                    aGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCg                                                                                                                                                                                                                                         >  2:367043/1‑288 (MQ=255)

                                      cATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCg                                                                                                                                                                                                                                                                    <  1:2899007/259‑1 (MQ=255)

                                       aTTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCca                                                                                                                                                                                                                                      <  2:274495/288‑1 (MQ=255)

                                                     cGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGcc                                                                                                                                                                                                                                       <  2:110061/273‑1 (MQ=255)

                                                                  gCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACa                                                                                                                                                                                                           <  2:307901/288‑1 (MQ=255)

                                                                  gCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACa                                                                                                                                                                                                           <  1:2941762/288‑1 (MQ=255)

                                                                  gCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCATGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTTCATCCGGAGATTATTTCAATGTTTTGTCCTCGTGGTGCTGCACAACTGGGTATCTATCGCCATCATCCTACACATCTTCACGATTTagag                                                                                                                                                                                                          >  2:2849697/1‑289 (MQ=255)

                                                                      gATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGAc                                                                                                                                                                                                      >  1:108154/1‑289 (MQ=255)

                                                                           aCCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGt                                                                                                                                                                                                                                                                         >  1:1107044/1‑217 (MQ=255)

                                                                           aCCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGt                                                                                                                                                                                                                                                 <  1:892572/241‑1 (MQ=255)

                                                                           aCCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGt                                                                                                                                                                                                                                                 >  2:892572/1‑241 (MQ=255)

                                                                                cTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGtat                                                                                                                                                                                                                                               <  2:2053445/238‑1 (MQ=255)

                                                                                         tGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGaa                                                                                                                                                                                                                                                          <  1:1766389/218‑1 (MQ=255)

                                                                                         tGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACg                                                                                                                                                                                                                  >  2:2524050/1‑258 (MQ=255)

                                                                                         tGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGG                                                                                                                                                                                   >  2:548055‑M1/1‑289 (MQ=255)

                                                                                                      ccAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTC                                                                                                                                                                                       <  1:3198813‑M1/272‑1 (MQ=255)

                                                                                                           tGGCGTTATATGACCTTCTTGCCCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatga                                                                                                                                                                   <  1:973784‑M1/287‑7 (MQ=255)

                                                                                                           tGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatga                                                                                                                                                                   <  1:3101357‑M1/287‑7 (MQ=255)

                                                                                                               gTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaa                                                                                                                                                              <  1:120686‑M1/288‑12 (MQ=255)

                                                                                                               gTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaa                                                                                                                                                              <  2:1255061‑M1/288‑12 (MQ=255)

                                                                                                                  atatGAGCTTATTGAACAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCC‑GAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattatt                                                                                                                                                         <  1:2789927‑M1/289‑17 (MQ=255)

                                                                                                                        ccTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTTCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatt                                                                                                                                                      >  1:1000071‑M1/1‑268 (MQ=255)

                                                                                                                        ccTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGaa                                                                                                                                                                                                                                                          <  1:601987/187‑1 (MQ=255)

                                                                                                                        ccTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattattt                                                                                                                                                     >  1:1899280‑M1/1‑268 (MQ=255)

                                                                                                                           tATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCg                                                                                                                                                                                                                                                                    <  2:462161/174‑1 (MQ=255)

                                                                                                                           tATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccg                                                                                                                                                 >  2:199207‑M1/1‑265 (MQ=255)

                                                                                                                           tATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccg                                                                                                                                                 >  1:1862528‑M1/1‑265 (MQ=255)

                                                                                                                            aTTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCca                                                                                                                                                                                                                                      >  1:761825/1‑203 (MQ=255)

                                                                                                                            aTTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccgg                                                                                                                                                >  1:787807‑M1/1‑264 (MQ=255)

                                                                                                                             ttGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggt                                                                                                                                               >  1:665733‑M1/1‑263 (MQ=255)

                                                                                                                                 ccAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttgg                                                                                                                                            <  1:2593752‑M1/288‑30 (MQ=255)

                                                                                                                                  cAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggtt                                                                                                                                          >  1:1159301‑M1/1‑258 (MQ=255)

                                                                                                                                  cAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGC‑AAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggtta                                                                                                                                         >  2:2435948‑M1/1‑257 (MQ=255)

                                                                                                                                   aaCTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCg                                                                                                                                                                                                                                                                    >  2:604122/1‑166 (MQ=255)

                                                                                                                                   aaCTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGcc                                                                                                                                                                                                                                       >  2:291264/1‑195 (MQ=255)

                                                                                                                                     cTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTggg                                                                                                                                                                                                                                                  >  1:708915/1‑182 (MQ=255)

                                                                                                                                     cTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaa                                                                                                                                                              <  1:3131978‑M1/266‑12 (MQ=255)

                                                                                                                                     cTGCATCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGaaca                                                                                                                                                                                                                                                        <  2:425447/176‑1 (MQ=255)

                                                                                                                                          tcgtGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatga                                                                                                                                                                   <  2:1910168‑M1/256‑7 (MQ=255)

                                                                                                                                           cgtGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccggga                                                                                                                                  <  2:1135677‑M1/288‑40 (MQ=255)

                                                                                                                                               aaCTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcat                                                                                                                              >  2:684548‑M1/1‑245 (MQ=255)

                                                                                                                                                aCTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatg                                                                                                                             <  2:2137577‑M1/288‑45 (MQ=255)

                                                                                                                                                             gTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccg                                                                                                                <  2:723462‑M1/288‑58 (MQ=255)

                                                                                                                                                                       tttCTCACGATCTGCATCTGGTGATGGC‑AAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctg                                                                                                     <  1:874326‑M1/288‑69 (MQ=255)

                                                                                                                                                                        ttCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgg                                                                                                    >  1:2048421‑M1/1‑220 (MQ=255)

                                                                                                                                                                         tctcACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattg                                                                                                                                                                <  2:2582341‑M1/228‑10 (MQ=255)

                                                                                                                                                                           tcACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggtt                                                                                                 <  2:180535‑M1/289‑73 (MQ=255)

                                                                                                                                                                                 tCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttgg                                                                                                                                            >  1:396147‑M1/1‑211 (MQ=255)

                                                                                                                                                                                     cATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtag                                                                                        >  1:2245937‑M1/1‑207 (MQ=255)

                                                                                                                                                                                     cATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatg                                                                                                                             >  2:3008635‑M1/1‑207 (MQ=255)

                                                                                                                                                                                           gTGATGGCAAAAACCGCTGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATGTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTCTCTATCGCCATAATCATAACCATCGTCTCGATTTACAGGGACGCATTGTTTTGCGTCGGGGCAATGATCGCccatgattgacttattatttcccggtttgttcgccgggatcatgctcgcctgtgcctcgggtacgcgtggttcgtttgtagtctggcg                                                                                 >  2:2787947‑M1/1‑201 (MQ=255)

                                                                                                                                                                                           gTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAggg                                                                                                                                                                                                        <  2:3139481/170‑1 (MQ=255)

                                                                                                                                                                                           gTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttgg                                                                                                                                            >  1:3010386‑M1/1‑201 (MQ=255)

                                                                                                                                                                                                ggCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGA                                                                                                                                                                             >  2:1736210‑M1/1‑192 (MQ=255)

                                                                                                                                                                                                  cAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAAT                                                                                                                                                                               <  2:1300248‑M1/188‑1 (MQ=255)

                                                                                                                                                                                                   aaaaaCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCg                                                                                                                                                                                                                                                                    >  1:2843862/1‑102 (MQ=255)

                                                                                                                                                                                                     aaaCCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtct                                                                       >  2:1151858‑M1/1‑191 (MQ=255)

                                                                                                                                                                                                               aGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtg                                                             <  1:1487008‑M1/289‑109 (MQ=255)

                                                                                                                                                                                                               aGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggt                                                              <  2:3226443‑M1/288‑108 (MQ=255)

                                                                                                                                                                                                                 tGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtg                                                             <  1:552544‑M1/287‑109 (MQ=255)

                                                                                                                                                                                                                   cTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatac                                                         >  1:1398674‑M1/1‑177 (MQ=255)

                                                                                                                                                                                                                    tgtgCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacg                                                        <  2:1688797‑M1/289‑114 (MQ=255)

                                                                                                                                                                                                                      tgCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGAT                                                                                                                                                                            >  1:2351903‑M1/1‑171 (MQ=255)

                                                                                                                                                                                                                           gAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCgtggtg                                                                                                                                                                                                                                                               <  2:189047/83‑1 (MQ=255)

                                                                                                                                                                                                                           gAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtcggtcttatttcggtgatacgctggctc                                                 >  2:490888‑M1/1‑169 (MQ=255)

                                                                                                                                                                                                                           gAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttattt                                                                  >  1:136600‑M1/1‑169 (MQ=255)

                                                                                                                                                                                                                           gAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccg                                                                                                        >  2:2107191‑M1/1‑169 (MQ=255)

                                                                                                                                                                                                                            attcaCCACATTTGTTGTTCCGGAAAGCCGGAAGTTGTTTCCCTGCATCCGGTGTTTATTTCACTGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctca                                                <  2:786432‑M1/287‑122 (MQ=255)

                                                                                                                                                                                                                              tCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctcatg                                              <  1:2089241‑M1/289‑124 (MQ=255)

                                                                                                                                                                                                                                       ttgttgTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACg                                                                                                                                                                                                                  >  2:2363780/1‑116 (MQ=255)

                                                                                                                                                                                                                                              tCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctcatgcctcattacttggcg                               <  1:193635‑M1/288‑139 (MQ=255)

                                                                                                                                                                                                                                               ccGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccggga                                                                                                                                  >  2:2642424‑M1/1‑149 (MQ=255)

                                                                                                                                                                                                                                               ccGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccggga                                                                                                                                  <  1:2642424‑M1/188‑40 (MQ=255)

                                                                                                                                                                                                                                                  gCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctcatgcctcattacttggcgtcgcg                          >  2:2274074‑M1/1‑146 (MQ=255)

                                                                                                                                                                                                                                                  gCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCt                                                                                                                                                                        <  2:2316304‑M1/147‑2 (MQ=255)

                                                                                                                                                                                                                                                  gCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCt                                                                                                                                                                        >  1:2316304‑M1/1‑146 (MQ=255)

                                                                                                                                                                                                                                                          gAAGTTGTTTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgcccgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcggtggtcttatttcggttgtactgtggctcctcccccgttacttggccctccctttggttt                  >  2:1043799‑M1/1‑138 (MQ=255)

                                                                                                                                                                                                                                                             gttgttTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACg                                                                                                                                                                                                                  >  2:1388302/1‑94 (MQ=255)

                                                                                                                                                                                                                                                                gttTCCCTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctcatgcctcattacttggcgtcgcgtttggtttgtt               >  1:2535195‑M1/1‑132 (MQ=255)

                                                                                                                                                                                                                                                                     ccTGCATCCTGATTTTTTTTCAATGTTTGGTCCTCTTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctcatgcctcattacttggcgtcgcgtttggtttgttgctggacg       <  2:85692‑M1/289‑163 (MQ=255)

                                                                                                                                                                                                                                                                     ccTGCATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctcatgcctcattacttggcgtcgcgtttggtttgttgctggacg       <  2:312786‑M1/289‑163 (MQ=255)

                                                                                                                                                                                                                                                                         cATCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctcatgcctcattacttggcgtcgcgtttggtttgttgctggacgtgaa   >  2:758309‑M1/1‑123 (MQ=255)

                                                                                                                                                                                                                                                                          aTCCGGAGTTTATTTCAATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCtcatgattgaattattatttcccggttggttagccgggatcatgctcgcctgtgccgcgggtccgctgggttcgtttgtagtctggcgtcgtatgtcttatttcggtgatacgctggctcatgcctcattacttggcgtcgcgtttggtttgttgctggacgtgaat  <  1:1599358‑M1/289‑168 (MQ=255)

|||

CTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAAATGGCCAGGTGGCGTTATATGACCTTATTGACCAACTGCGTCGTGAACTGGATTGTGGCGTTTTAATGGTTTCTCACGATCTGCATCTGGTGATGGCAAAAACCGATGAAGTGCTGTGCCTGAATCACCACATTTGTTGTTCCGGCACACCGGAAGTTGTTTCCCTGCATCCGGAGTTTATTAGTATGTTTGGTCCTCGTGGTGCTGAACAACTGGGTATCTATCGCCATCATCATAACCATCGTCACGATTTACAGGGACGAATTGTTTTGCGTCGGGGAAATGATCGCAGTTGATTTAACGTCGGGGAAATGATCGCTCATGATTGAATTATTATTTCCCGGTTGGTTAGCCGGGATCATGCTCGCCTGTGCCGCGGGTCCGCTGGGTAGCTTTGTAGTCTGGCGTCGTATGTCTTATTTCGGTGATACGCTGGCTCATGCCAGTTTACTTGGCG  >  CP042583/1623743‑1624297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A