Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,513,137 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,513,1370GC100.0% 852.6 / NA 356intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (178/178);  total (178/178)
*CP0425831,513,1380CG99.7% 846.6 / NA 356intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (178/177);  total (178/178)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,513,1390TA99.7% 848.7 / NA 356intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (177/178);  total (178/178)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 356 total aligned reads displayed.

ACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCGCTGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCG  >  CP042583/1512862‑1513416
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                     
 cATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCCCGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGAtt                                                                                                                                                                                                                                                                           <  2:29952/289‑1 (MQ=255)
   ttGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTAc                                                                                                                                                                                                                                                                         <  2:3031445/289‑1 (MQ=255)
              cgcAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAggt                                                                                                                                                                                                                                                               <  1:3077962/288‑1 (MQ=255)
                         ccacGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGt                                                                                                                                                                                                                                                   >  1:1375421/1‑289 (MQ=255)
                               gtccACCCGCAAGTTGTACCATCCCGCCTGTGTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAAACAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAGACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCaa                                                                                                                                                                                                                                              <  2:2737726/286‑1 (MQ=255)
                                       gCAAACAGTACCATCCAGCCGGCTTTCGCCGCTTCCTGAGAGCTCTGTTGCGCTTGCCCGGCGGAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGtcatca                                                                                                                                                                                                                                     <  2:2661227/289‑1 (MQ=255)
                                               tACCCTCACGCTGCCTTTCGCCGCTGCGGGAGAGACCTGTTCAGCTGACCCTGCGTAAGCGGCCAGCAGTAAACAGCCATTCAGCAAACCTCAGCCACGAACTTCACTGAACAAGCCATAGCGGTGATTAATAGTATTAAGACGCGCAACAAGCCAGTCGTGACGCTGGTTAACG‑CCATTAAGCAGCTCTGGTGTGTTGGTGGGCTCCAGCACTGTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGgcg                                                                                                                                                                                                                             <  2:1840899/289‑1 (MQ=255)
                                                    tCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCAGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCAct                                                                                                                                                                                                                         >  2:2332038/1‑288 (MQ=255)
                                                      gcGCCTGCTTTCGCCGCTTCCTGGGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCtt                                                                                                                                                                                                                      <  1:1774632/288‑1 (MQ=255)
                                                         ccTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGTTGGTGCCATGAGTCCCAACGGTCATCCCGCGGGCGCCCTCTTCgg                                                                                                                                                                                                                   >  2:546266/1‑289 (MQ=255)
                                                         ccTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCgg                                                                                                                                                                                                                   >  1:835836/1‑289 (MQ=255)
                                                                       ttCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCa                                                                                                                                                                                                     <  2:219584/289‑1 (MQ=255)
                                                                                      tttCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCaa                                                                                                                                                                                                                                              >  1:558759/1‑234 (MQ=255)
                                                                                           cTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACcgccgc                                                                                                                                                                                  >  2:764079/1‑288 (MQ=255)
                                                                                           cTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTG‑CACCGCCGAGGCCAg                                                                                                                                                                                                                                                                                 <  2:784556/192‑1 (MQ=255)
                                                                                                       aaTCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTgg                                                                                                                                                                      <  1:688548/288‑1 (MQ=255)
                                                                                                             cATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTa                                                                                                                                                                                                                                                                          <  2:2522726/182‑1 (MQ=255)
                                                                                                                    tACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGa                                                                                                                                                        >  1:1342459/1‑289 (MQ=255)
                                                                                                                     acacAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGAt                                                                                                                                                       >  1:585956/1‑289 (MQ=255)
                                                                                                                       acaGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTaaactgtc                                                                                                                                                      >  1:1869287/1‑282 (MQ=255)
                                                                                                                         aGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTgg                                                                                                                                                                      >  2:1135163/1‑270 (MQ=255)
                                                                                                                         aGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTgg                                                                                                                                                                      <  1:1135163/270‑1 (MQ=255)
                                                                                                                         aGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCg                                                                                                                                                                    >  2:1604801/1‑272 (MQ=255)
                                                                                                                           ccAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGtcatca                                                                                                                                                                                                                                     >  1:1310830/1‑205 (MQ=255)
                                                                                                                           ccAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCCGCGCTTTCGCGGCAGTGAACACATCCGGc                                                                                                                                                  >  2:2457097/1‑288 (MQ=255)
                                                                                                                           ccAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGc                                                                                                                                                  >  2:1407322/1‑288 (MQ=255)
                                                                                                                           ccAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCg                                                                                                                                                 >  2:1308442/1‑289 (MQ=255)
                                                                                                                              aTCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTgg                                                                                                                                                                      >  1:1658182/1‑265 (MQ=255)
                                                                                                                              aTCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTaa                                                                                                                                                            <  2:693031/275‑1 (MQ=255)
                                                                                                                              aTCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTc                                                                                                                                               >  1:979569/1‑288 (MQ=255)
                                                                                                                                  aaatagacagaccacgAACTTCAATCAACAAACCCGTGCGATGATTAATATTATTTAGCCGCTCAACAAACCACTCTTTACGCTGTTTACCG‑CCATTAAGCATCTCTGGTGTGTTGATTAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGcc                                                                                                                                          <  2:1565150/275‑1 (MQ=255)
                                                                                                                                     aaCCTAAGCCTCGAACTTCACTGAACAACACATAGCGCTGATTAAGAGTATTAAGACGCTCAACAACCCCGACGTGCCGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTTATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTa                                                                                                                                       <  2:585956/289‑1 (MQ=255)
                                                                                                                                        cTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTa                                                                                                                                       <  1:2520012/286‑1 (MQ=255)
                                                                                                                                          aaGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCa                                                                                                                                  <  1:764079/289‑1 (MQ=255)
                                                                                                                                           aGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTCGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTg                                                                                                                                    <  1:696953/286‑1 (MQ=255)
                                                                                                                                                cgAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCCGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCCACCGGTCAAGCGCCGCCCAGGCCTTTGGCGGGAGTGAACAGATCAAGCTCAACGCCGTacgccctatag                                                                                                                             >  2:2042128/1‑278 (MQ=255)
                                                                                                                                                 gAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCg                                                                                                                                                                    >  1:1017747/1‑248 (MQ=255)
                                                                                                                                                 gAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCg                                                                                                                                                                    <  2:1017747/248‑1 (MQ=255)
                                                                                                                                                  aaCTTCACTGAACAAACCATAGCGGTGAGTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCAGCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTgg                                                                                                                                                                      <  2:1901328/245‑1 (MQ=255)
                                                                                                                                                   aCTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCa                                                                                                                          <  1:297017/288‑1 (MQ=255)
                                                                                                                                                       cACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATAAGGCGTCACGCCGTAGTGCata                                                                                                                                >  1:1565150/1‑278 (MQ=255)
                                                                                                                                                                 aCCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCa                                                                                                                                                                                                     <  1:2679826/199‑1 (MQ=255)
                                                                                                                                                                 aCCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTg                                                                                                                                    <  1:2738213/264‑1 (MQ=255)
                                                                                                                                                                      cgCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATa                                                                                                                        <  2:3100797/270‑1 (MQ=255)
                                                                                                                                                                      aGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCtt                                                                                                                                                                         >  2:2812091/1‑222 (MQ=255)
                                                                                                                                                                      aGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCtt                                                                                                                                                                         <  1:2812091/222‑1 (MQ=255)
                                                                                                                                                                      aGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCgg                                                                                                                                                                   <  2:3192288/228‑1 (MQ=255)
                                                                                                                                                                      aGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGccc                                                                                                      >  1:1439116/1‑289 (MQ=255)
                                                                                                                                                                      aGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATAAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCg                                                                                                                                         <  2:405109/254‑1 (MQ=255)
                                                                                                                                                                           tGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTgg                                                                                                                                                                      >  2:149528/1‑220 (MQ=255)
                                                                                                                                                                           tGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGc                                                                                                                           >  1:1053961/1‑263 (MQ=255)
                                                                                                                                                                           tGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACg                                                                                                  <  2:1347700/288‑1 (MQ=255)
                                                                                                                                                                           tGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACg                                                                                                  >  1:2890941/1‑288 (MQ=255)
                                                                                                                                                                           tGATTAAGGTTATTAAGACGCTCAACAAACCAGTCGTGACGCTGGTTAACG‑CCATTAGGCATGTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCGGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACg                                                                                                  <  2:2890941/288‑1 (MQ=255)
                                                                                                                                                                              ttAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCgcgtgat                                                                                               <  1:284127/288‑7 (MQ=255)
                                                                                                                                                                                     tATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCa                                                                                                                                              <  1:924173/234‑1 (MQ=255)
                                                                                                                                                                                     tATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGt                                                                                                                                     <  2:2848750/243‑1 (MQ=255)
                                                                                                                                                                                       ttAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTGGAACt                                                                                      >  1:12275/1‑288 (MQ=255)
                                                                                                                                                                                          aGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTtcat                                                                                  <  1:2055338/289‑1 (MQ=255)
                                                                                                                                                                                               cTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACTACGCacc                                                                                                                                                                                                   <  2:104159/171‑1 (MQ=255)
                                                                                                                                                                                               cTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCa                                                                                                                                  >  2:1282672/1‑236 (MQ=255)
                                                                                                                                                                                               cTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCa                                                                                                                                  <  1:1282672/236‑1 (MQ=255)
                                                                                                                                                                                               cTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATCTAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCaaaa                                                                             >  2:638830/1‑289 (MQ=255)
                                                                                                                                                                                               cTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCa                                                                                                                          >  2:118711/1‑244 (MQ=255)
                                                                                                                                                                                               cTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCaaaa                                                                             >  1:1004498/1‑289 (MQ=255)
                                                                                                                                                                                               cTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTATTCGGTAGCCAACAACGCACCGACAGGGAAACCGCCGACCAGCGCTTTGGCGGGAGTTAACAg                                                                                                                                                         >  1:329126/1‑213 (MQ=255)
                                                                                                                                                                                                 caacaaACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCa                                                                                                                                              >  1:219753/1‑222 (MQ=255)
                                                                                                                                                                                                    caaACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCaa                                                                        <  2:2425989/289‑1 (MQ=255)
                                                                                                                                                                                                            tCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCAt                                                                >  2:921542/1‑289 (MQ=255)
                                                                                                                                                                                                              gTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAg                                                                                                                                                                              >  1:2434302/1‑177 (MQ=255)
                                                                                                                                                                                                              gTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCa                                                                                                                                  <  1:1421447/221‑1 (MQ=255)
                                                                                                                                                                                                                            cG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGcc                                                                                                                                                                                                                                                                     >  2:1972738/1‑76 (MQ=255)
                                                                                                                                                                                                                                  ttAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCg                                                                                                                                                 >  2:337799/1‑187 (MQ=255)
                                                                                                                                                                                                                                  ttAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCg                                                                                                                                                 <  1:337799/187‑1 (MQ=255)
                                                                                                                                                                                                                                  ttAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCata                                                                                                                                >  2:1419872/1‑204 (MQ=255)
                                                                                                                                                                                                                                  ttAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCgcgtga                                                                                                >  1:3038188/1‑231 (MQ=255)
                                                                                                                                                                                                                                  ttAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCa                                            >  2:2062852/1‑288 (MQ=255)
                                                                                                                                                                                                                                     aGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCa                                        <  1:2290479/289‑1 (MQ=255)
                                                                                                                                                                                                                                       cATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGa                                      <  2:720555/289‑1 (MQ=255)
                                                                                                                                                                                                                                        aTCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTgg                                                           >  1:2096358/1‑267 (MQ=255)
                                                                                                                                                                                                                                         tctcTGGTGTGTTGAGTAGCTCCAGCACTTTGCCCGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGGGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGAcc                                    <  2:1439116/289‑1 (MQ=255)
                                                                                                                                                                                                                                          ctctGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCtt                                                                                                                                                                         >  1:2583377/1‑155 (MQ=255)
                                                                                                                                                                                                                                          ctctGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACg                                                                                                                                            <  1:1511835/184‑1 (MQ=255)
                                                                                                                                                                                                                                          ctctGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGAcc                                    >  2:2578456/1‑288 (MQ=255)
                                                                                                                                                                                                                                                    ttGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGTGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCata                                                                                                                                <  1:1806913/186‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTcccc                                                                                                               >  1:1924033/1‑203 (MQ=255)
                                                                                                                                                                                                                                                    ttGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTaa                              >  1:1997788/1‑284 (MQ=255)
                                                                                                                                                                                                                                                    ttGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAAc                          >  1:398930/1‑288 (MQ=255)
                                                                                                                                                                                                                                                     tgatgaGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGtcat                                                                                                                                                                                                                                       <  1:2485523/82‑1 (MQ=255)
                                                                                                                                                                                                                                                       atgaGCTCCACCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACGTCATCAAAAATCAACAGCGCATTGAGGCGGGTACACAATTCACGCAAACACTGTAACCAcgctt                      >  2:2274351/1‑287 (MQ=255)
                                                                                                                                                                                                                                                         gaGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGAGGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTg                    <  1:3167104/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          aGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACa                                                                      <  1:1699685/238‑1 (MQ=255)
                                                                                                                                                                                                                                                            cTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACg                                                                                                  >  2:1760877/1‑208 (MQ=255)
                                                                                                                                                                                                                                                                     tttGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCgcgtgat                                                                                               >  1:735015/1‑196 (MQ=255)
                                                                                                                                                                                                                                                                       tgcctgccACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTTACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGcaccac      >  1:483438/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       tgcctgccACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAAc                          >  1:2154619/1‑269 (MQ=255)
                                                                                                                                                                                                                                                                       tgcctgccACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAAc                          <  2:2154619/269‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tgcctgccACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCACACCTTTGAAAAACGCGTTCCTGGCTGGcaccac      >  2:2486500/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       tgcctgccACCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGGTACACAATTCACGCCGAACTTTGAAAAACCCCGTGCTGGCCGGcaccc       >  2:1771874/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                        gcctgccCCCGCCGAGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCATCGCTTTGGCGGTAGTTAACAGATCAGGCGTCCCGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACcaca     <  1:2381317/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                     
ACATTGAGCGCAGGCGCAAAACGCACCACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACG‑CCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCGCTGGCCAGCGGATTACCGCCATAGGTGGTGCCATGAGTGCCAACGGTCATCACGCGGGCGCACTCTTCGGTTGCCAACAACGCACCGACCGGGAAACCGCCGCCCAGCGCTTTGGCGGTAGTTAACAGATCAGGCGTCACGCCGTAGTGCATATAGGCATACAGTTCCCCGGTGCGCCCGACGCCGGTTTGTACTTCATCAAAAATCAACAGCGCATTGTGGCGGTTACACAATTCACGCAGACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCG  >  CP042583/1512862‑1513416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A