Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,510,186 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,510,1860GC100.0% 453.2 / NA 194intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (100/94);  total (100/94)
*CP0425831,510,1870CG98.5% 435.8 / NA 194intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (98/92);  total (100/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,510,1880TA99.0% 433.1 / NA 194intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (99/91);  minor base G (0/2);  total (100/94)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 194 total aligned reads displayed.

TGCCGCATTGGCTACCCACATTGGGCTAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAAGCTCAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAAGCTGTCGCTCTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCT  >  CP042583/1509916‑1510465
                                                                                                                                                                                                                                                                              |||                                                                                                                                                                                                                                                                                      
 gCCGCATTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAatgat                                                                                                                                                                                                                                                                       >  1:550302/1‑289 (MQ=255)
    gagttGGCTACCCCCATGTGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTAGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCg                                                                                                                                                                                                                                                                    <  2:138161/286‑1 (MQ=255)
    gCATTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCg                                                                                                                                                                                                                                                                    <  1:799916/289‑1 (MQ=255)
      aTTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTc                                                                                                                                                                                                                                                                  >  1:302284/1‑289 (MQ=255)
      aTTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCCCCGCCAGTCGCGGGTTCGACACCTGAAAACGGGTACGGGTAGAGGCTTCATTACCAAACGACCGGCCCGCGTAATGATGCgc                                                                                                                                                                                                                                                                   >  2:1771124/1‑287 (MQ=255)
        tGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAg                                                                                                                                                                                                                                                                <  1:1163644/289‑1 (MQ=255)
              cccACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGGGACGGGTAGAGGCTTCATTACCAAACGACAGGACCGCGTAAGTAGGCGTCAGCCCTa                                                                                                                                                                                                                                                           >  2:1552882/1‑288 (MQ=255)
                   attggcgaaGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGGCGCGGGTTAGATCCCTGAAAACGGTGACCGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGTGGCCACCCCACCAGc                                                                                                                                                                                                                                                      >  2:1386476/9‑288 (MQ=255)
                     tggcgaaGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCCCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGGCGCGGGTTAGACACCTGGAAACGGTGACGGGTAGAGGCTTCATTACCCAACCAACGGCCCGCGTCATGAAGCCTCAGCCCATCCAGccc                                                                                                                                                                                                                                                    >  2:1784080/7‑288 (MQ=255)
                        cgaaGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGTGTCAGCCCTACCAGCCCGTCg                                                                                                                                                                                                                                                >  1:1265071/4‑289 (MQ=255)
                        cgaaGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAACGATGCGTCCGCCCTACCAGccccgcc                                                                                                                                                                                                                                                >  2:931111/4‑285 (MQ=255)
                             cGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAAtt                                                                                                                                                                                                                                           >  1:357226/1‑289 (MQ=255)
                              ggAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAAtt                                                                                                                                                                                                                                           <  1:1376564/288‑1 (MQ=255)
                                aaCTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGa                                                                                                                                                                                                                                         <  1:1768396/288‑1 (MQ=255)
                                       gCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCCGCCCTACCAGCCCCTCCAAATTGCCTTCCCAg                                                                                                                                                                                                                                 >  2:126135/1‑289 (MQ=255)
                                                 cAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCCAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCAt                                                                                                                                                                                                                         <  1:1084781/287‑1 (MQ=255)
                                                   gTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCAGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCCGCATCCCTACCAGCCAGTCGAAATTGACTTCCCAGGCATCCATCGc                                                                                                                                                                                                                      >  2:361643/1‑288 (MQ=255)
                                                              ggCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCATCCCGTCGCAATTGCCTTCCCAGGCGTTCATAGCACCACCCCATCg                                                                                                                                                                                                          >  2:1127775/1‑289 (MQ=255)
                                                              ggCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGTACCACCtgtcc                                                                                                                                                                                                           >  1:1729763/1‑284 (MQ=255)
                                                                   gCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGaaaa                                                                                                                                                                                                     >  1:1354021/1‑289 (MQ=255)
                                                                     aaCTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCACGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGaaaaa                                                                                                                                                                                                    >  1:533414/1‑288 (MQ=255)
                                                                      aCTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATc                                                                                                                                                                                                  <  1:610662/289‑1 (MQ=255)
                                                                           ttCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACTGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGcc                                                                                                                                                                                             <  1:741839/289‑1 (MQ=255)
                                                                           ttCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGcc                                                                                                                                                                                             <  1:1313998/289‑1 (MQ=255)
                                                                            tCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAACCGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTNGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGccc                                                                                                                                                                                            <  1:9/289‑1 (MQ=255)
                                                                            tCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGccc                                                                                                                                                                                            <  1:1195755/289‑1 (MQ=255)
                                                                                  aCCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCggg                                                                                                                                                                                         <  1:931111/286‑1 (MQ=255)
                                                                                  aCCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGt                                                                                                                                                                                       <  2:1830219/288‑1 (MQ=255)
                                                                                     tGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGcc                                                                                                                                                                                                                                                                                  >  1:1894885/1‑194 (MQ=255)
                                                                                      gCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGc                                                                                                                                                                                  >  1:1481352/1‑289 (MQ=255)
                                                                                       cTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCggg                                                                                                                                                                                         <  2:1171150/281‑1 (MQ=255)
                                                                                           tCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGc                                                                                                                                                                                                                                                      <  2:436295/216‑1 (MQ=255)
                                                                                           tCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGc                                                                                                                                                                                                                                                      >  1:436295/1‑216 (MQ=255)
                                                                                           tCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCCTCGCCACACCCCACCCGACAAAGCCCACCCGGGGTTAAGCGTgcg                                                                                                                                                                             >  2:470969/1‑287 (MQ=255)
                                                                                              cTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGccc                                                                                                                                                                                            >  1:27803/1‑271 (MQ=255)
                                                                                              cTACCACTAAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGccc                                                                                                                                                                                            <  2:27803/271‑1 (MQ=255)
                                                                                               tACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCggg                                                                                                                                                                          <  1:725869/288‑1 (MQ=255)
                                                                                               tACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCACCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCggg                                                                                                                                                                          <  2:241529/288‑1 (MQ=255)
                                                                                                      gAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTTATCGCCCCACCTCCTCGGAAAAATCCAGCCCCGGGTTAACTGTGGGGTGCAATggtc                                                                                                                                                                  >  2:1993767/1‑286 (MQ=255)
                                                                                                         tCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTaacg                                                                                                                                                               >  1:1013004/1‑287 (MQ=255)
                                                                                                         tCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCCCACCACCTCATCGGAAAAATCCAGCCCCGGGTTAAGCGTGGCGGGCAATGGTaact                                                                                                                                                               >  2:69210/1‑287 (MQ=255)
                                                                                                         tCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCAGTGCACCACCCCATCGCAAAAAGCCAGCCCGGGGTTAAGCTTGGCGGGCAGTGTTaaaa                                                                                                                                                               >  2:1461921/1‑287 (MQ=255)
                                                                                                                 gACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCTCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGAGGGGCGGGCAATGGTaacgagtccga                                                                                                                                                        >  2:340592/1‑279 (MQ=255)
                                                                                                                   cGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTc                                                                                                                                                                                                                           <  2:175048/219‑1 (MQ=255)
                                                                                                                                    cggccgccggTGGGGCGGGATCACGGCCTGGGGGAATCCCGCATGGGCAAGGGCTTTAATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGAGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑cc                                                                                                                                   <  2:222700/280‑1 (MQ=255)
                                                                                                                                    cGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑cc                                                                                                                                   <  1:967993/289‑1 (MQ=255)
                                                                                                                                       acgtgagTGAGGCGGGATCCCGGCATGGGTGAAGCCCGCGTGGGCAAGGTGTTTCATGTGCAGTAAGACCTGCTTAGCCGCCAGGCGCGGGTTAGACACCTGAAGACGGTGAAGGGTAGGGGATTCATTAGCAAGCGACAGGCCCGCGGAATGATGCGTCAGCCCTACCAGCACGTCGAAAGTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAGATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCAt                                                                                                                                 <  2:1281026/282‑1 (MQ=255)
                                                                                                                                       aCGCTCGGGCGGCGGGACCACGGCCTGGGGTAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTACGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAACCGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCAt                                                                                                                                 <  1:1941891/288‑1 (MQ=255)
                                                                                                                                        gggtCGGGCGGGGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATgc                                                                                                                               <  2:1481352/286‑1 (MQ=255)
                                                                                                                                            cGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAg                                                                                                                           <  1:1054400/289‑1 (MQ=255)
                                                                                                                                             gTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCCGGCAATGTTAACGACTCCGACTCCCGGCTCGCCAACGGCCCATCCGCAg                                                                                                                           >  2:1874008/1‑289 (MQ=255)
                                                                                                                                             gTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCCCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGGGTCCGACTCCAGGCCCTCCATCAG‑CCATGCGCCGt                                                                                                                          >  2:196914/1‑289 (MQ=255)
                                                                                                                                               gcggcgGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTaa                                                                                                                        >  1:1426299/1‑289 (MQ=255)
                                                                                                                                               gcggcgGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTa                                                                                                                         <  1:1127775/288‑1 (MQ=255)
                                                                                                                                                gcaagggATCAGGGCCTGGGGCGATCCGGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTG‑AAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAAt                                                                                                                       <  2:1932408/284‑1 (MQ=255)
                                                                                                                                                 ggcggGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCACGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAAt                                                                                                                       <  2:533414/288‑1 (MQ=255)
                                                                                                                                                     ggATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTc                                                                                                                                                                                                                                                                  <  1:1023817/146‑1 (MQ=255)
                                                                                                                                                     ggATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTaac                                                                                                                                                                <  1:1781557/244‑2 (MQ=255)
                                                                                                                                                            ggCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATAcc                                                                                                           <  1:1461921/289‑1 (MQ=255)
                                                                                                                                                             gCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAATCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCCG‑CCATGCGCAGTTATCCGCGCCATAcaa                                                                                                          >  2:1082654/1‑287 (MQ=255)
                                                                                                                                                                 gggggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCCCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGgc                                                                                                       >  1:255417/1‑288 (MQ=255)
                                                                                                                                                                 gggggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGGTAAGCGTGGCGGGCAATGTTAAAGAGTCCGACCCCAGGCCCGCCATCGG‑CCAGGCGCCGTCATCTGCCGCACCCCAgcc                                                                                                       >  2:839031/1‑286 (MQ=255)
                                                                                                                                                                 gggggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCAACTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTACGGCATACCAGgc                                                                                                       >  1:1540944/1‑288 (MQ=255)
                                                                                                                                                                  ggggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCcgcg                                                                                                                                                                                                                                                                              >  1:1462226/1‑121 (MQ=255)
                                                                                                                                                                  ggggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCcgcg                                                                                                                                                                                                                                                                              <  2:1462226/121‑1 (MQ=255)
                                                                                                                                                                  ggggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCCTACCAGccg                                                                                                      >  2:129285/1‑288 (MQ=255)
                                                                                                                                                                  ggggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATAcc                                                                                                           <  2:1663462/283‑1 (MQ=255)
                                                                                                                                                                  ggggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATAcc                                                                                                           >  1:1663462/1‑283 (MQ=255)
                                                                                                                                                                   gggAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGgc                                                                                                       >  1:1028780/1‑286 (MQ=255)
                                                                                                                                                                    ggAATCCCGCATCGGGAAGGGCCTTCATTTTCAGTAAGCCCTTCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCt                                                                                                    <  2:27526/288‑1 (MQ=255)
                                                                                                                                                                        tCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGaa                                                                                                                                                                                                       >  2:880446/1‑186 (MQ=255)
                                                                                                                                                                          ccGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGa                                                                                             <  1:15753/289‑1 (MQ=255)
                                                                                                                                                                                  gCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTAcc                                                                                     >  2:1433526/1‑289 (MQ=255)
                                                                                                                                                                                             cATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCaa                                                                          <  1:221939/289‑1 (MQ=255)
                                                                                                                                                                                                      tAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGGTACCGGAAGCACCAATGCCGCCGa                                                                 >  2:992906/1‑289 (MQ=255)
                                                                                                                                                                                                        aGCCATGCTGGGCTGCCAAGCGCCGGTGAGACACCCGAAAACGGTGACGGGTAGAGCCTTCATAACCCAACGACAGGCCGGCGTAGTGATGAGCCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGGTAAGCGTGGCGGGCAATGTTAACGAGTCCGACGCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGaa                                                                <  2:780672/288‑1 (MQ=255)
                                                                                                                                                                                                          cccTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGcc                                                                                                                                                                                             <  2:552245/162‑1 (MQ=255)
                                                                                                                                                                                                            cTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGgcg                                                           <  1:1386476/289‑1 (MQ=255)
                                                                                                                                                                                                            cTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGgcg                                                           <  1:1985401/289‑1 (MQ=255)
                                                                                                                                                                                                            cTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGgcg                                                           <  1:1637594/289‑1 (MQ=255)
                                                                                                                                                                                                              gCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAgg                                                                                                        <  1:181101/242‑1 (MQ=255)
                                                                                                                                                                                                                ttCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGt                                                       <  1:839031/289‑1 (MQ=255)
                                                                                                                                                                                                                ttCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGt                                                       <  2:1013004/289‑1 (MQ=255)
                                                                                                                                                                                                                  cgccgcCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTAc                                                     >  2:794701/1‑289 (MQ=255)
                                                                                                                                                                                                                   gccgccGGTCGAGGGTTAGCAACCTGAAAACGGTGACGGGTAGAGGCTTAATTACCAAACGGCAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCGGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACt                                                    <  1:275870/289‑1 (MQ=255)
                                                                                                                                                                                                                     cgccAGTAGCGGGTTAGACAACGGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGACCGAGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACGTCCCAGGCGTTCATCGCACCACTGCAGCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTgg                                                  <  2:1608860/289‑1 (MQ=255)
                                                                                                                                                                                                                           tCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGgcagca                                             >  1:1288738/1‑288 (MQ=255)
                                                                                                                                                                                                                                          cTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGt                             >  2:905561/1‑289 (MQ=255)
                                                                                                                                                                                                                                          cTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGCCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGCCGCCGTACTGCCAGCCACGGTAAGCGGttt                               >  2:1979886/1‑287 (MQ=255)
                                                                                                                                                                                                                                             aaaaCGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTcc                          >  1:511943/1‑289 (MQ=255)
                                                                                                                                                                                                                                              aaaCGGTGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCa                         <  1:947437/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    tcaCGGGTAGAGGCTTCATTGCCAAACGCCAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTaa                    <  2:290742/286‑1 (MQ=255)
                                                                                                                                                                                                                                                    tGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTaa                    <  1:698883/288‑1 (MQ=255)
                                                                                                                                                                                                                                                    tGACGGGTAGAGGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAAc                   >  2:1406816/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              agGCTTCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCcccgc          >  1:1787300/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                  ttCATTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGACAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCcgcg     <  1:330800/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ctCATTACCAAACCACCGGCCCGCGCAAGTGTGCGTCAGCCCTACCGGCCCGTGAAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTGAAGCGGGGCGGGCAATGTTAACGGGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCcgcg     <  2:1028780/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                  atccgtACCAAACGGCAGGCCCGCGTCATGATGCGTCAGCCCTCCCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCcgcg     <  2:1354021/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aTTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGcc   >  1:31322/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                     aTTACCAAACGACAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAACGAGTCCGACTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCt  <  1:1082654/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              |||                                                                                                                                                                                                                                                                                      
TGCCGCATTGGCTACCCACATTGGGCTAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACGCAGCACCGGAATAAACGGACGCTCGTGCGGCGGGATCACGGCCTGGGGGAATCCCGCATCGGCAAGGGCTTTCATTTTCAGTAAGCCCTGCTTCGCCGCCAGTCGCGGGTTAGACACCTGAAAACGGTGACGGGTAGAGGCTTCATTACCAAAGCTCAGGCCCGCGTAATGATGCGTCAGCCCTACCAGCCCGTCGAAATTGACTTCCCAGGCGTTCATCGCACCACCTCATCGGAAAAATCCAGCCCGGGGTTAAGCGTGGCGGGCAATGTTAAGCTGTCGCTCTCCAGGCTCGCCATCGG‑CCATGCGCAGTAATCTGCGGCATACCAGGCGCTGGGGCGATGGTTACCGGAAGCACCAATGCCGCCGAATGGCGCGGTACTGGCAGCACCGGTAAGCGGTTTGTTCCAGTTAACAATCCCCGCCCGCGCCT  >  CP042583/1509916‑1510465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A